Team:Heidelberg/Modeling/miBSdesigner

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Revision as of 14:45, 25 October 2010

BS designer for miRNA

Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)

Perfect Binding Site
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8)

Customized MISMATCH position

Supplementary region

Customized MATCH positions (input numbers from 9 to 22)

Modify AU content

A in position -1

A in position 0

A in position 1

A in position 8

A in position 9

A in position 10

Sticky ends for integration in plasmid:

Customized: 5' 3'

Construction of microRNA binding sites

Name of primer 1

Sequence of primer 1

Name of primer 2

Sequence of primer 2

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