Team:Heidelberg/Modeling/miBSdesigner

From 2010.igem.org

(Difference between revisions)
Line 15: Line 15:
div.suboption {text-indent:80px;}
div.suboption {text-indent:80px;}
div.clear {clear: both;}
div.clear {clear: both;}
-
div.reswrap2 {width: 550px; padding:25px; border-width:5px; margin:auto; border-style:solid; border-color:#11526f; height: 240px; text-align:left; background-color:#ffaf58; display:none;}
+
div.reswrap2 {width: 550px; padding:25px; border-width:5px; margin:auto; border-style:solid; border-color:#11526f; height: 240px; text-align:left; background-color:#ffaf58; display:none; font-size:12pt;}
 +
.variaresult {font-size: 12pt; color: #11526f; font-weight: bold;}
</style>
</style>
Line 91: Line 92:
<br>
<br>
<div class="title">
<div class="title">
-
<span style="font-size: 18.0px; text-decoration: underline;">
+
<span style="font-size: 14pt; text-decoration: underline;">
Modify AU content </span>
Modify AU content </span>
</div>
</div>
Line 104: Line 105:
<div class="clear">
<div class="clear">
<br>
<br>
-
<div class="option"><span style="font-size: 18.0px; text-decoration: underline;">Sticky ends for integration in plasmid: </span></div>
+
<div class="option"><span style="font-size: 18px; text-decoration: underline;">Sticky ends for integration in plasmid: </span></div>
<div class="option">  <SELECT id="stickyends" name="stickyends" onchange="chMd3()">
<div class="option">  <SELECT id="stickyends" name="stickyends" onchange="chMd3()">
<OPTION SELECTED value="none">None
<OPTION SELECTED value="none">None
Line 122: Line 123:
<br>
<br>
<div class="reswrap2" id="resultdiv">
<div class="reswrap2" id="resultdiv">
-
<span>Name of primer 1</span><br>
+
Name of primer 1<br>
-
<span id="NamA" style="font-size: 18.0px; color: #11526f; font-weight: bold;"></span><br>
+
<span id="NamA" class="variaresult"></span><br>
-
<span>Sequence of primer 1</span><br>
+
Sequence of primer 1<br>
-
<span id="seqA" style="font-size: 18.0px; color: #11526f; font-weight: bold;"></span><br>
+
<span id="seqA" class="variaresult"></span><br>
-
<span>Name of primer 2</span><br>
+
Name of primer 2<br>
-
<span id="NamB" style="font-size: 18.0px; color: #11526f; font-weight: bold;"></span><br>
+
<span id="NamB" class="variaresult""></span><br>
-
<span>Sequence of primer 2</span><br>
+
Sequence of primer 2<br>
-
<span id="seqB" style="font-size: 18.0px; color: #11526f; font-weight: bold;"></span><br>
+
<span id="seqB" class="variaresult"></span><br>
</div>
</div>
</body>
</body>

Revision as of 01:17, 25 October 2010

BS designer for miRNA


Construction of microRNA binding sites
Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)

Perfect Binding Site
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8)
Customized MISMATCH position

Supplementary region
Customized MATCH positions (input numbers from 9 to 22)


Modify AU content
A in position -1
A in position 0
A in position 1
A in position 8
A in position 9
A in position 10


Sticky ends for integration in plasmid:
Customized: 5' 3'


Name of primer 1

Sequence of primer 1

Name of primer 2

Sequence of primer 2

Retrieved from "http://2010.igem.org/Team:Heidelberg/Modeling/miBSdesigner"