Team:Heidelberg/Modeling/miBSdesigner

From 2010.igem.org

(Difference between revisions)
Line 23: Line 23:
<div class="wrapper2">
<div class="wrapper2">
<center>
<center>
-
<span style="font-family: Arial; color: #11526f; font-size: 18.0px; font-weight: bold;">
+
<span style="font-family: Arial; color: #11526f; font-size: 18.0px; text-decoration: underline; font-weight: bold;">
-
Construction of microRNA binding sites </span><br>
+
Construction of microRNA binding sites </span><br><br>
<span style="font-size: 17.0px;">Sequence name </span>
<span style="font-size: 17.0px;">Sequence name </span>
<br>
<br>
Line 124: Line 124:
<div class="wrapper">
<div class="wrapper">
<img style="border-width: 0px;" src="https://static.igem.org/mediawiki/2010/c/c0/MiBSdesigner.png" width="260" height="147" />
<img style="border-width: 0px;" src="https://static.igem.org/mediawiki/2010/c/c0/MiBSdesigner.png" width="260" height="147" />
-
<br><br>
+
<br><br><br><br>
<br><br><br>
<br><br><br>
<div class="reswrap2" id="resultdiv">
<div class="reswrap2" id="resultdiv">

Revision as of 18:59, 25 October 2010

BS designer for miRNA
Construction of microRNA binding sites

Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)


Perfect Binding Site
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8)
Customized MISMATCH position

Supplementary region
Customized MATCH positions (input numbers from 9 to 22)


Modify AU content
A in position -1
A in position 0
A in position 1
A in position 8
A in position 9
A in position 10


Sticky ends for integration in plasmid:
Customized: 5' 3'









Name of primer 1


Sequence of primer 1


Name of primer 2


Sequence of primer 2

Retrieved from "http://2010.igem.org/Team:Heidelberg/Modeling/miBSdesigner"