Team:Heidelberg/Modeling/miBSdesigner

From 2010.igem.org

(Difference between revisions)
Line 1: Line 1:
{{:Team:Heidelberg/Template}}
{{:Team:Heidelberg/Template}}
-
{{:Team:Heidelberg/Pagetop|home}}
+
{{:Team:Heidelberg/Pagetop|mibsdesigner}}
<!--<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">-->
<!--<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">-->
<html>
<html>

Revision as of 09:01, 24 October 2010

BS designer for miRNA

Construction of microRNA binding sites
Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)

Perfect Binding Site
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8)
Customized MISMATCH position

Supplementary region
Customized MATCH positions (input numbers from 9 to 22)


Modify AU content
A in position -1
A in position 0
A in position 1
A in position 8
A in position 9
A in position 10


Sticky ends for integration in plasmid:
Customized: 5' 3'


Name of primer 1

Sequence of primer 1

Name of primer 2

Sequence of primer 2