Team:Heidelberg/Modeling/miBSdesigner

From 2010.igem.org

(Difference between revisions)

Revision as of 18:50, 26 October 2010

BS designer for miRNA

Construction of microRNA binding sites

Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)
?

Perfect Binding Site
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8) ?

Customized MISMATCH position

Supplementary region ?

Customized MATCH positions

Modify AU content ?

A in position -1
A in position 1
A in position 9

A in position 0
A in position 8
A in position 10

Sticky ends for integration into plasmid ?

Customized:
5'
3'

Name of primer 1

Sequence of primer 1

Name of primer 2

Sequence of primer 2

@@ Line 34: / Line 34: @@
 		Spacer (inert sequence)<br>
 		<input type="text" name="spacer" id="spacer" size="40" value="TTATATTTTATGACA"/>
-		</div></center>
+		<span class="help"><a href="#">?</a></span></div></div></center>
 		<br><br>
 	<div class="largetext">
@@ Line 49: / Line 49: @@
 				<td width=320px>
 					<div class="title">
-						Seed sequence (1-8)</div>
+						Seed sequence (1-8) &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span class="help"><a href="#">?</a></span>
+					</div>
 					<div class="option"><SELECT name="seed" onchange="chMd1()" disabled="disabled">
 						<OPTION value="0">
@@ Line 65: / Line 66: @@
 				<td>
 					<div class="title">
-						Supplementary region</div>
+						Supplementary region &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span class="help"><a href="#">?</a></span></div>
 					<div class="option"><SELECT name="comp" onchange="chMd2()" disabled="disabled">
 						<OPTION value="0">
@@ Line 86: / Line 87: @@
 				<td>
 					<div class="title">
-						Modify AU content</div>
+						Modify AU content &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span class="help"><a href="#">?</a></span></div>
 					<table style="line-height:2; font-size:12px;">
 						<tr>
@@ Line 103: / Line 104: @@
 				</td>
 				<td>
-			<div class="title">Sticky ends for integration into plasmid</div>
+			<div class="title">Sticky ends for integration into plasmid &nbsp; <span class="help"><a href="#">?</a></span></div></div>
 			<div class="option"><SELECT id="stickyends" name="stickyends" onchange="chMd3()">
 			<OPTION SELECTED value="none">None