Team:Heidelberg

From 2010.igem.org

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<div id="wrapperheadline">iGEM Heidelberg Mission 2010: miBricks</div>
<div id="wrapperheadline">iGEM Heidelberg Mission 2010: miBricks</div>
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<div id="projectabstract">The great potential of gene therapy is currently limited by two major challenges:  tissue specific gene delivery and regulation of gene expression, either dependent on cell-specific properties or intentionally independent of the cellular context. We followed two synergistic tracks to address these problems.<br>
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One, we have developed a novel method for miRNA based gene expression tuning in mammalian cells, allowing the fine-tuning of gene expression based on synthetic miRNAs, as well as the cell specific on- and off-targeting based on endogenous miRNAs. We show that this method is functional in vivo and in vitro and prove the high potential of all three miRNA-based regulation approaches.<br>
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Two, we have developed a standardized and fast approach towards the creation of AAV-based gene delivery vectors. We have achieved exceptionally selective tissue-specific targeting in vitro and in vivo with hepatocyte specific delivery vectors.<br>
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We are happy to provide the synthetic biology community with two high impact innovations which will fuel the improvement of tissue specific gene therapy approaches and other medical applications of synthetic biology.</div><br>
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<div id="slider">
<div id="slider">
<a href="http://2010.igem.org/Team:Heidelberg/Project/Capsid_Shuffling"><img src="http://2010.igem.org/wiki/images/e/ed/Slide_CapShuffling.png" alt="" rel="http://2010.igem.org/wiki/images/3/30/Slide_CapShuffling_thumb.png"/></a>
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<a class="piclinks" href="javascript:writeText('And we are not talking about fashion! In our team, a group of students was in charge of the computational interpretation and modeling of the data generated. Want to read more? Click <a href=&quot;http://2010.igem.org/Team:Heidelberg/Modeling&quot;>here</a>!')" onMouseOver="mouseOver(1)"  onMouseOut="mouseOut(1)" onMouseDown="mouseDown(1)" onMouseUp="mouseUp(1)">
 
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<a class="piclinks" href="javascript:writeText('Our team comprised a group of twenty motivated students from eight different countries, two instructors and eight advisors. Want to meet them? Click <a href=&quot;http://2010.igem.org/Team:Heidelberg/Team&quot;>here</a>!')" onMouseOver="mouseOver(2)" onMouseOut="mouseOut(2)" onMouseDown="mouseDown(2)" onMouseUp="mouseUp(2)">
 
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<a class="piclinks" href="javascript:writeText('A philosophy and a psychology student helped us to understand how Synthetic Biology interacts with society. Click <a href=&quot;http://2010.igem.org/Team:Heidelberg/Human_Practices&quot;>here</a> to see how our science is percieved by the public!')" onMouseOver="mouseOver(3)" onMouseOut="mouseOut(3)" onMouseDown="mouseDown(3)" onMouseUp="mouseUp(3)">
 
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<center><table id="desc-table"><tr><td><div id ="desc" border=0><center>Please click a Button to get more information! </center></div></td></tr></table></center>
 
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<a class="piclinks" href="javascript:writeText('As in any piece of research, we have been keeping detailed notebooks with the experiments we have done. Want to retrace our steps? Click <a href=&quot;http://2010.igem.org/Team:Heidelberg/Notebook&quot;>here</a>!')" onMouseOver="mouseOver(4)" onMouseOut="mouseOut(4)" onMouseDown="mouseDown(4)" onMouseUp="mouseUp(4)">
 
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<a class="piclinks" href="javascript:writeText('We have generated more than 100 parts that have been sent to iGEM headquarters. Want to know which ones? Click <a href=&quot;http://2010.igem.org/Team:Heidelberg/Parts&quot;>here</a>!')" onMouseOver="mouseOver(5)" onMouseOut="mouseOut(5)" onMouseDown="mouseDown(5)" onMouseUp="mouseUp(5)">
 
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<a class="piclinks" href="javascript:writeText('Our team was sponsored by a non-profit organization, nine companies and three academic sponsors. Want to meet them? Click <a href=&quot;http://2010.igem.org/Team:Heidelberg/Support&quot;>here</a>!')" onMouseOver="mouseOver(6)" onMouseOut="mouseOut(6)" onMouseDown="mouseDown(6)" onMouseUp="mouseUp(6)">
 
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<div id="projectabstract"><span class="t3">The key to successful gene therapy</span><br>... is integration of tissue specificity and fine-tuned target gene expression. iGEM Team Heidelberg 2010 unlocks the world of synthetic microRNAs. We engineered a toolkit for standardized measurements of interactions between artificial miRNAs and their binding sites. Thus, the expression level of any gene of choice could be arbitrarily adjusted by employing the corresponding binding site design. To produce tissue specific miRNA gene shuttles, we developed an evolution-based method for synthesis of new adeno associated viruses. In the future, miBricks could open the doors to new Synthetic Biology based medical approaches. </div><br><br><br>
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Revision as of 23:40, 27 October 2010

iGEM Heidelberg Mission 2010: miBricks
The great potential of gene therapy is currently limited by two major challenges: tissue specific gene delivery and regulation of gene expression, either dependent on cell-specific properties or intentionally independent of the cellular context. We followed two synergistic tracks to address these problems.
One, we have developed a novel method for miRNA based gene expression tuning in mammalian cells, allowing the fine-tuning of gene expression based on synthetic miRNAs, as well as the cell specific on- and off-targeting based on endogenous miRNAs. We show that this method is functional in vivo and in vitro and prove the high potential of all three miRNA-based regulation approaches.
Two, we have developed a standardized and fast approach towards the creation of AAV-based gene delivery vectors. We have achieved exceptionally selective tissue-specific targeting in vitro and in vivo with hepatocyte specific delivery vectors.
We are happy to provide the synthetic biology community with two high impact innovations which will fuel the improvement of tissue specific gene therapy approaches and other medical applications of synthetic biology.





... and way more important: