Team:Harvard/vectors/parts

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Revision as of 23:18, 26 October 2010 by Pmboyle (Talk | contribs)



parts and primers

vectors

For more information on the vectors visit our "meet the vectors" page

NameBiobrick NameOriginal NameDescriptionResistanceResistance (Plants)Sequence
V1-pORE Open Series 1 (O1)Agrobacterium vector open seriesKanamycinpatlink
V2-pORE Open Series 2 (O2)Agrobacterium vector open seriesKanamycinnptIIlink
V3-pORE Expression Series 3 (E3)Agrobacterium vector with ENTCUP2 promoterKanamycinpatlink
V4-pORE Expression Series 4 (E4)Agrobacterium vector with ENTCUP2 promoterKanamycinnptIIlink
V5-pORE Reporter Series 1 (R1)Agrobacterium vector with gusA reporterKanamycinnptIIlink
V6-pORE Reporter Series 3 (R3)Agrobacterium vector with smgfp reporterKanamycinnptIIlink
V7BBa_K382000
-
Agrobacterium vector open series with biobrick cloning siteKanamycinpatlink
V8BBa_K382001
-
Agrobacterium vector open series with biobrick cloning siteKanamycinnptIIlink
V9BBa_K382002
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinpatlink
V10BBa_K382003
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinnptIIlink
V11BBa_K382004
-
Agrobacterium vector with gusA reporter and biobrick cloning siteKanamycinnptIIlink
V12BBa_K382005
-
Agrobacterium vector with smgfp reporter and biobrick cloning siteKanamycinnptIIlink


primers

NameDescriptionSequence
VP3HindIII-pENTCUP2 forward primer for Expression Series insertlink
VP4pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insertlink
VP5HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insertlink
VP6gusA-NheI reverse primer for Reporter Series 1 insertlink
VP7HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insertlink
VP8smGFP-NheI reverse primer for Reporter Series 1 insertlink
VP9Biobrick multiple cloning site for annealing with VP10 to create Open Series Insertlink
VP11Biobrick multiple cloning site for annealing with VP10 to create Open Series Insertlink
VP121 of 5 for sequencing V12 biobrick cloning site and smgfp reporterlink
VP132 of 5 for sequencing V12 biobrick cloning site and smgfp reporterlink
VP143 of 5 for sequencing V12 biobrick cloning site and smgfp reporterlink
VP154 of 5 for sequencing V12 biobrick cloning site and smgfp reporterlink
VP165 of 5 for sequencing V12 biobrick cloning site and smgfp reporterlink
VP171 of 9 for sequencing V11 biobrick cloning site and gusA reporterlink
VP182 of 9 for sequencing V11 biobrick cloning site and gusA reporterlink
VP193 of 9 for sequencing V11 biobrick cloning site and gusA reporterlink
VP204 of 9 for sequencing V11 biobrick cloning site and gusA reporterlink
VP215 of 9 for sequencing V11 biobrick cloning site and gusA reporterlink
VP226 of 9 for sequencing V11 biobrick cloning site and gusA reporterlink
VP237 of 9 for sequencing V11 biobrick cloning site and gusA reporterlink
VP248 of 9 for sequencing V11 biobrick cloning site and gusA reporterlink
VP259 of 9 for sequencing V11 biobrick cloning site and gusA reporterlink
VP261 of 5 for sequencing V9 and V10 biobrick cloning site and promoterlink
VP272 of 5 for sequencing V9 and V10 biobrick cloning site and promoterlink
VP283 of 5 for sequencing V9 and V10 biobrick cloning site and promoterlink
VP294 of 5 for sequencing V9 and V10 biobrick cloning site and promoterlink
VP305 of 5 for sequencing V9 and V10 biobrick cloning site and promoterlink
VP311 of 2 for sequencing V7 and V8 biobrick cloning sitelink
VP342 of 2 for sequencing V7 and V8 biobrick cloning sitelink