Team:Harvard/vectors/parts

From 2010.igem.org

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<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr>
<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr>
<tr><td>VP3</td><td>HindIII-pENTCUP2 forward primer for Expression Series insert</td><td></td></tr>
<tr><td>VP3</td><td>HindIII-pENTCUP2 forward primer for Expression Series insert</td><td></td></tr>
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<tr><td>VP4</td><td>pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert</td><td><a href="https://2010.igem.org/Team:Harvard/VP3>link</a></td></tr>
+
<tr><td>VP4</td><td>pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert</td><td><a href="https://2010.igem.org/Team:Harvard/VP4">link</a></td></tr>
<tr><td>VP5</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td></td></tr>
<tr><td>VP5</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td></td></tr>
<tr><td>VP6</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>
<tr><td>VP6</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>

Revision as of 18:28, 24 October 2010



parts and primers

vectors

For more information on the vectors visit our "meet the vectors" page

NameOriginal NameDescriptionResistanceResistance (Plants)Sequence
V1pORE Open Series 1 (O1)Agrobacterium vector open seriesKanamycinpatlink
V2pORE Open Series 2 (O2)Agrobacterium vector open seriesKanamycinnptIIlink
V3pORE Expression Series 3 (E3)Agrobacterium vector with ENTCUP2 promoterKanamycinpatlink
V4pORE Expression Series 4 (E4)Agrobacterium vector with ENTCUP2 promoterKanamycinnptIIlink
V5pORE Reporter Series 1 (R1)Agrobacterium vector with gusA reporterKanamycinnptIIlink
V6pORE Reporter Series 3 (R3)Agrobacterium vector with smgfp reporterKanamycinnptIIlink
V7
-
Agrobacterium vector open series with biobrick cloning siteKanamycinpatlink
V8
-
Agrobacterium vector open series with biobrick cloning siteKanamycinnptIIlink
V9
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinpatlink
V10
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinnptIIlink
V11
-
Agrobacterium vector with gusA reporter and biobrick cloning siteKanamycinnptIIlink
V12
-
Agrobacterium vector with smgfp reporter and biobrick cloning siteKanamycinnptIIlink


primers

NameDescriptionSequence
VP3HindIII-pENTCUP2 forward primer for Expression Series insert
VP4pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insertlink
VP5HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert
VP6gusA-NheI reverse primer for Reporter Series 1 insert
VP7HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert
VP8smGFP-NheI reverse primer for Reporter Series 1 insert
VP9Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert
VP11Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert
VP121 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP132 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP143 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP154 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP165 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP171 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP182 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP193 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP204 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP215 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP226 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP237 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP248 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP259 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP261 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP272 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP283 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP294 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP305 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP331 of 2 for sequencing V7 and V8 biobrick cloning site
VP342 of 2 for sequencing V7 and V8 biobrick cloning site