http://2010.igem.org/wiki/index.php?title=Team:Harvard/vectors&feed=atom&action=historyTeam:Harvard/vectors - Revision history2024-03-29T12:14:42ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Harvard/vectors&diff=168985&oldid=prevMinniss at 23:45, 26 October 20102010-10-26T23:45:32Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 23:45, 26 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 7:</td>
<td colspan="2" class="diff-lineno">Line 7:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="abstract"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="abstract"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>abstract</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>abstract</h1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Our aim in creating the iGarden was to create a framework for plant engineering following the BioBrick standard. We began by modifying a set of <a href="https://2010.igem.org/Team:Harvard/vectors/vectors">vectors</a> designed to facilitate agrobacterium-mediated transformation to be compatible with the BioBrick standard, and creating a collection of constructs to be expressed in plants - such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, and <a href="https://2010.igem.org/Team:Harvard/fences">containment</a> mechanisms. We built all constructs in E. coli, and once complete, cloned them into the BioBrick agrobacterium vectors. We then transformed agrobacteria with our constructs via electroporation, and subsequently transformed <i>Arabidopsis</i> seeds by dipping flowers into the transgenic agrobacteria. In our final step, we harvested the transformed seeds and screened them for our designed constructs using antibiotic selection.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Our aim in creating the iGarden was to create a framework for plant engineering following the BioBrick standard. We began by modifying a set of <a href="https://2010.igem.org/Team:Harvard/vectors/vectors">vectors</a> designed to facilitate agrobacterium-mediated transformation to be compatible with the BioBrick standard, and creating a collection of constructs to be expressed in plants - such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, and <a href="https://2010.igem.org/Team:Harvard/fences">containment</a> mechanisms. We built all constructs in <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i></ins>, and once complete, cloned them into the BioBrick agrobacterium vectors. We then transformed agrobacteria with our constructs via electroporation, and subsequently transformed <i>Arabidopsis</i> seeds by dipping flowers into the transgenic agrobacteria. In our final step, we harvested the transformed seeds and screened them for our designed constructs using antibiotic selection.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2010/0/03/Harvard2010roadmap.png" width="600px"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2010/0/03/Harvard2010roadmap.png" width="600px"></div></td></tr>
</table>Minnisshttp://2010.igem.org/wiki/index.php?title=Team:Harvard/vectors&diff=168938&oldid=prevMinniss at 23:41, 26 October 20102010-10-26T23:41:47Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 23:41, 26 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 7:</td>
<td colspan="2" class="diff-lineno">Line 7:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="abstract"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="abstract"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>abstract</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>abstract</h1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Our aim in creating the iGarden was to create a framework for plant engineering following the BioBrick standard. We began by modifying a set of <a href="https://2010.igem.org/Team:Harvard/vectors/vectors">vectors</a> designed to facilitate agrobacterium-mediated transformation to be compatible with the BioBrick standard, and creating a collection of constructs to be expressed in plants - such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, and <a href="https://2010.igem.org/Team:Harvard/fences">containment</a> mechanisms. We built all constructs in E. coli, and once complete, cloned them into the BioBrick agrobacterium vectors. We then transformed agrobacteria with our constructs via electroporation, and subsequently transformed <del class="diffchange diffchange-inline">arabidopsis </del>seeds by dipping flowers into the transgenic agrobacteria. In our final step, we harvested the transformed seeds and screened them for our designed constructs using antibiotic selection.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Our aim in creating the iGarden was to create a framework for plant engineering following the BioBrick standard. We began by modifying a set of <a href="https://2010.igem.org/Team:Harvard/vectors/vectors">vectors</a> designed to facilitate agrobacterium-mediated transformation to be compatible with the BioBrick standard, and creating a collection of constructs to be expressed in plants - such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, and <a href="https://2010.igem.org/Team:Harvard/fences">containment</a> mechanisms. We built all constructs in E. coli, and once complete, cloned them into the BioBrick agrobacterium vectors. We then transformed agrobacteria with our constructs via electroporation, and subsequently transformed <ins class="diffchange diffchange-inline"><i>Arabidopsis</i> </ins>seeds by dipping flowers into the transgenic agrobacteria. In our final step, we harvested the transformed seeds and screened them for our designed constructs using antibiotic selection.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2010/0/03/Harvard2010roadmap.png" width="600px"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2010/0/03/Harvard2010roadmap.png" width="600px"></div></td></tr>
</table>Minnisshttp://2010.igem.org/wiki/index.php?title=Team:Harvard/vectors&diff=122772&oldid=prevJyquinn at 02:59, 23 October 20102010-10-23T02:59:02Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:59, 23 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 2:</td>
<td colspan="2" class="diff-lineno">Line 2:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><html<del class="diffchange diffchange-inline">><style>a{color:white}</style</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><html></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="maincontent"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="maincontent"></div></td></tr>
</table>Jyquinnhttp://2010.igem.org/wiki/index.php?title=Team:Harvard/vectors&diff=122767&oldid=prevJyquinn at 02:58, 23 October 20102010-10-23T02:58:43Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:58, 23 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 2:</td>
<td colspan="2" class="diff-lineno">Line 2:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><html></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><html<ins class="diffchange diffchange-inline">><style>a{color:white}</style</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="maincontent"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="maincontent"></div></td></tr>
</table>Jyquinnhttp://2010.igem.org/wiki/index.php?title=Team:Harvard/vectors&diff=122545&oldid=prevJyquinn at 01:56, 23 October 20102010-10-23T01:56:08Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 01:56, 23 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 7:</td>
<td colspan="2" class="diff-lineno">Line 7:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="abstract"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="abstract"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>abstract</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>abstract</h1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Our aim in creating the iGarden was to create a framework for plant engineering following the BioBrick standard. We began by modifying a set of <a href="https://2010.igem.org/Team:Harvard/vectors/vectors">vectors</a> designed to facilitate agrobacterium-mediated transformation to be compatible with the BioBrick standard, and creating a collection of constructs to be expressed in plants - such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, and <a href="https://2010.igem.org/Team:Harvard/fences">containment</a> mechanisms. We built <del class="diffchange diffchange-inline">the </del>constructs in E. coli, and once complete, cloned them into the BioBrick agrobacterium vectors. We then transformed agrobacteria with our constructs via electroporation, and subsequently transformed arabidopsis seeds by dipping flowers into the transgenic agrobacteria. In our final step, we harvested the transformed seeds and screened them for our designed constructs using antibiotic selection.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Our aim in creating the iGarden was to create a framework for plant engineering following the BioBrick standard. We began by modifying a set of <a href="https://2010.igem.org/Team:Harvard/vectors/vectors">vectors</a> designed to facilitate agrobacterium-mediated transformation to be compatible with the BioBrick standard, and creating a collection of constructs to be expressed in plants - such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, and <a href="https://2010.igem.org/Team:Harvard/fences">containment</a> mechanisms. We built <ins class="diffchange diffchange-inline">all </ins>constructs in E. coli, and once complete, cloned them into the BioBrick agrobacterium vectors. We then transformed agrobacteria with our constructs via electroporation, and subsequently transformed arabidopsis seeds by dipping flowers into the transgenic agrobacteria. In our final step, we harvested the transformed seeds and screened them for our designed constructs using antibiotic selection.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2010/0/03/Harvard2010roadmap.png" width="600px"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2010/0/03/Harvard2010roadmap.png" width="600px"></div></td></tr>
</table>Jyquinnhttp://2010.igem.org/wiki/index.php?title=Team:Harvard/vectors&diff=122543&oldid=prevJyquinn at 01:55, 23 October 20102010-10-23T01:55:30Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 01:55, 23 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 7:</td>
<td colspan="2" class="diff-lineno">Line 7:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="abstract"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="abstract"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>abstract</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>abstract</h1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Our aim in creating the iGarden was to create a framework for plant engineering <del class="diffchange diffchange-inline">using </del>the BioBrick standard. <del class="diffchange diffchange-inline">To do so, we created </del>a set of vectors <del class="diffchange diffchange-inline">fitting the BioBrick standard into which we place out BioBrick constructs with which we can transfer the constructs from E. coli, our assembly chassis, </del>to <del class="diffchange diffchange-inline">the plant, our final chassis. We make this transfer via </del>agrobacterium-mediated transformation<del class="diffchange diffchange-inline">.</p></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Our aim in creating the iGarden was to create a framework for plant engineering <ins class="diffchange diffchange-inline">following </ins>the BioBrick standard. <ins class="diffchange diffchange-inline">We began by modifying </ins>a set of <ins class="diffchange diffchange-inline"> <a href="https://2010.igem.org/Team:Harvard/</ins>vectors<ins class="diffchange diffchange-inline">/vectors">vectors</a> designed </ins>to <ins class="diffchange diffchange-inline">facilitate </ins>agrobacterium-mediated transformation <ins class="diffchange diffchange-inline">to be compatible </ins>with the <ins class="diffchange diffchange-inline">BioBrick standard, and creating a collection </ins>of constructs <ins class="diffchange diffchange-inline">to be expressed </ins>in <ins class="diffchange diffchange-inline">plants </ins>- such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, <ins class="diffchange diffchange-inline">and </ins><a href="https://2010.igem.org/Team:Harvard/fences"><ins class="diffchange diffchange-inline">containment</ins></a> mechanisms. We built the <ins class="diffchange diffchange-inline">constructs in E</ins>. coli<ins class="diffchange diffchange-inline">, and once complete, cloned them </ins>into <ins class="diffchange diffchange-inline">the BioBrick agrobacterium </ins>vectors<ins class="diffchange diffchange-inline">. We then transformed agrobacteria with </ins>our <ins class="diffchange diffchange-inline">constructs via electroporation, and subsequently transformed arabidopsis seeds by dipping flowers </ins>into <ins class="diffchange diffchange-inline">the transgenic </ins>agrobacteria. <ins class="diffchange diffchange-inline">In our </ins>final step<ins class="diffchange diffchange-inline">, we harvested the transformed seeds </ins>and <ins class="diffchange diffchange-inline">screened them </ins>for our designed constructs <ins class="diffchange diffchange-inline">using antibiotic selection</ins>.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><p>The process began </del>with the <del class="diffchange diffchange-inline">creation </del>of <del class="diffchange diffchange-inline">desired </del>constructs in <del class="diffchange diffchange-inline">e. coli </del>- such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, <del class="diffchange diffchange-inline">or </del><a href="https://2010.igem.org/Team:Harvard/fences"><del class="diffchange diffchange-inline">fence</del></a> mechanisms. We <del class="diffchange diffchange-inline">next </del>built <del class="diffchange diffchange-inline">a set of <a href="https://2010.igem.org/Team:Harvard/vectors/vectors">plant vectors</a> compatible with </del>the <del class="diffchange diffchange-inline">BioBrick standard and moved our parts from e</del>. coli into <del class="diffchange diffchange-inline">these </del>vectors<del class="diffchange diffchange-inline">, which allowed us to electroporate </del>our <del class="diffchange diffchange-inline">parts </del>into agrobacteria. <del class="diffchange diffchange-inline">The </del>final step <del class="diffchange diffchange-inline">was to transform arabidopsis flowers with our engineered agrobacteria </del>and <del class="diffchange diffchange-inline">select </del>for <del class="diffchange diffchange-inline">plants containing </del>our designed constructs.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2010/0/03/Harvard2010roadmap.png" width="600px"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2010/0/03/Harvard2010roadmap.png" width="600px"></div></td></tr>
</table>Jyquinnhttp://2010.igem.org/wiki/index.php?title=Team:Harvard/vectors&diff=122502&oldid=prevJyquinn at 01:37, 23 October 20102010-10-23T01:37:19Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 01:37, 23 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 7:</td>
<td colspan="2" class="diff-lineno">Line 7:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="abstract"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="abstract"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>abstract</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>abstract</h1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Our <del class="diffchange diffchange-inline">goal is </del>the <del class="diffchange diffchange-inline">creation of an "</del>iGarden<del class="diffchange diffchange-inline">" with </del>the BioBrick standard. To <del class="diffchange diffchange-inline">approach this project</del>, we <del class="diffchange diffchange-inline">used agrobacterium-mediated transformation to move our </del>constructs <del class="diffchange diffchange-inline">from </del>the <del class="diffchange diffchange-inline">bacterium e</del>. coli <del class="diffchange diffchange-inline">to agrobacterium</del>, <del class="diffchange diffchange-inline">and finally into </del>the plant <del class="diffchange diffchange-inline">arabidopsis (and maybe in the future</del>, <del class="diffchange diffchange-inline">strawberries!)</del>.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Our <ins class="diffchange diffchange-inline">aim in creating </ins>the iGarden <ins class="diffchange diffchange-inline">was to create a framework for plant engineering using </ins>the BioBrick standard. To <ins class="diffchange diffchange-inline">do so</ins>, we <ins class="diffchange diffchange-inline">created a set of vectors fitting the BioBrick standard into which we place out BioBrick </ins>constructs <ins class="diffchange diffchange-inline">with which we can transfer </ins>the <ins class="diffchange diffchange-inline">constructs from E</ins>. coli, <ins class="diffchange diffchange-inline">our assembly chassis, to </ins>the plant, <ins class="diffchange diffchange-inline">our final chassis. We make this transfer via agrobacterium-mediated transformation</ins>.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The process began with the creation of desired constructs in e. coli - such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, or <a href="https://2010.igem.org/Team:Harvard/fences">fence</a> mechanisms. We next built a set of <a href="https://2010.igem.org/Team:Harvard/vectors/vectors">plant vectors</a> compatible with the BioBrick standard and moved our parts from e. coli into these vectors, which allowed us to electroporate our parts into agrobacteria. The final step was to transform arabidopsis flowers with our engineered agrobacteria and select for plants containing our designed constructs.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The process began with the creation of desired constructs in e. coli - such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, or <a href="https://2010.igem.org/Team:Harvard/fences">fence</a> mechanisms. We next built a set of <a href="https://2010.igem.org/Team:Harvard/vectors/vectors">plant vectors</a> compatible with the BioBrick standard and moved our parts from e. coli into these vectors, which allowed us to electroporate our parts into agrobacteria. The final step was to transform arabidopsis flowers with our engineered agrobacteria and select for plants containing our designed constructs.</p></div></td></tr>
</table>Jyquinnhttp://2010.igem.org/wiki/index.php?title=Team:Harvard/vectors&diff=101122&oldid=prevDevin at 17:23, 14 October 20102010-10-14T17:23:50Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 17:23, 14 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 7:</td>
<td colspan="2" class="diff-lineno">Line 7:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="abstract"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="abstract"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>abstract</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>abstract</h1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Our goal is the creation of an "iGarden" with the BioBrick standard. To approach this project, we used agrobacterium-mediated transformation to move our constructs from the bacterium e. coli to agrobacterium, and finally into the plant arabidopsis.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Our goal is the creation of an "iGarden" with the BioBrick standard. To approach this project, we used agrobacterium-mediated transformation to move our constructs from the bacterium e. coli to agrobacterium, and finally into the plant arabidopsis <ins class="diffchange diffchange-inline">(and maybe in the future, strawberries!)</ins>.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The process began with the creation of desired constructs in e. coli - such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, or <a href="https://2010.igem.org/Team:Harvard/fences">fence</a> mechanisms. We next built a set of <a href="https://2010.igem.org/Team:Harvard/vectors/vectors">plant vectors</a> compatible with the BioBrick standard and moved our parts from e. coli into these vectors, which allowed us to electroporate our parts into agrobacteria. The final step was to transform arabidopsis flowers with our engineered agrobacteria and select for plants containing our designed constructs.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The process began with the creation of desired constructs in e. coli - such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, or <a href="https://2010.igem.org/Team:Harvard/fences">fence</a> mechanisms. We next built a set of <a href="https://2010.igem.org/Team:Harvard/vectors/vectors">plant vectors</a> compatible with the BioBrick standard and moved our parts from e. coli into these vectors, which allowed us to electroporate our parts into agrobacteria. The final step was to transform arabidopsis flowers with our engineered agrobacteria and select for plants containing our designed constructs.</p></div></td></tr>
</table>Devinhttp://2010.igem.org/wiki/index.php?title=Team:Harvard/vectors&diff=101121&oldid=prevDevin at 17:23, 14 October 20102010-10-14T17:23:10Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 17:23, 14 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 9:</td>
<td colspan="2" class="diff-lineno">Line 9:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our goal is the creation of an "iGarden" with the BioBrick standard. To approach this project, we used agrobacterium-mediated transformation to move our constructs from the bacterium e. coli to agrobacterium, and finally into the plant arabidopsis.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our goal is the creation of an "iGarden" with the BioBrick standard. To approach this project, we used agrobacterium-mediated transformation to move our constructs from the bacterium e. coli to agrobacterium, and finally into the plant arabidopsis.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>The process began with the creation of desired constructs in e. coli - such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, or <a href="https://2010.igem.org/Team:Harvard/fences">fence</a> mechanisms. We next built a set of <a href="https://2010.igem.org/Team:Harvard/vectors/vectors">plant vectors</a> compatible with the BioBrick standard and moved our parts from e. coli into these vectors, which allowed us to electroporate our parts into <del class="diffchange diffchange-inline">agrobactera</del>. The final step was to transform arabidopsis flowers with our engineered agrobacteria and select for plants containing our designed constructs.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>The process began with the creation of desired constructs in e. coli - such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, or <a href="https://2010.igem.org/Team:Harvard/fences">fence</a> mechanisms. We next built a set of <a href="https://2010.igem.org/Team:Harvard/vectors/vectors">plant vectors</a> compatible with the BioBrick standard and moved our parts from e. coli into these vectors, which allowed us to electroporate our parts into <ins class="diffchange diffchange-inline">agrobacteria</ins>. The final step was to transform arabidopsis flowers with our engineered agrobacteria and select for plants containing our designed constructs.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2010/0/03/Harvard2010roadmap.png" width="600px"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2010/0/03/Harvard2010roadmap.png" width="600px"></div></td></tr>
</table>Devinhttp://2010.igem.org/wiki/index.php?title=Team:Harvard/vectors&diff=101120&oldid=prevDevin at 17:22, 14 October 20102010-10-14T17:22:14Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 17:22, 14 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 7:</td>
<td colspan="2" class="diff-lineno">Line 7:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="abstract"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="abstract"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>abstract</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>abstract</h1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Our goal is the creation of an "iGarden" with the BioBrick standard. <del class="diffchange diffchange-inline">For such a concept to work</del>, we used agrobacterium-mediated transformation to move our constructs from the bacterium e. coli into the plant arabidopsis.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Our goal is the creation of an "iGarden" with the BioBrick standard. <ins class="diffchange diffchange-inline">To approach this project</ins>, we used agrobacterium-mediated transformation to move our constructs from the bacterium e. coli <ins class="diffchange diffchange-inline">to agrobacterium, and finally </ins>into the plant arabidopsis.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>The process began with the creation of <del class="diffchange diffchange-inline">a set of </del><a href="https://2010.igem.org/Team:Harvard/<del class="diffchange diffchange-inline">vectors/vectors</del>"><del class="diffchange diffchange-inline">plant vectors</del></a> <del class="diffchange diffchange-inline">compatible with the BioBrick standard. To express our constucts - such as </del><a href="https://2010.igem.org/Team:Harvard/<del class="diffchange diffchange-inline">flavor</del>"><del class="diffchange diffchange-inline">flavor</del></a> <del class="diffchange diffchange-inline">elements</del>, <a href="https://2010.igem.org/Team:Harvard/<del class="diffchange diffchange-inline">allergy</del>"><del class="diffchange diffchange-inline">allergen</del></a> <del class="diffchange diffchange-inline">knockdown</del>, <a href="https://2010.igem.org/Team:Harvard/<del class="diffchange diffchange-inline">color</del>"><del class="diffchange diffchange-inline">pigment</del></a> <del class="diffchange diffchange-inline">accumulation, or </del><a href="https://2010.igem.org/Team:Harvard/<del class="diffchange diffchange-inline">fences</del>"><del class="diffchange diffchange-inline">fence</del></a> <del class="diffchange diffchange-inline">mechanisms, we cloned </del>the <del class="diffchange diffchange-inline">constucts </del>into <del class="diffchange diffchange-inline">the agrobacterium </del>vectors <del class="diffchange diffchange-inline">with E. coli as the chassis</del>, <del class="diffchange diffchange-inline">then transformed </del>into agrobacteria <del class="diffchange diffchange-inline">via electroporation, then innoculated our </del>plants <del class="diffchange diffchange-inline">with the contruct </del>containing <del class="diffchange diffchange-inline">agrobacteria</del>. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>The process began with the creation of <ins class="diffchange diffchange-inline">desired constructs in e. coli - such as </ins><a href="https://2010.igem.org/Team:Harvard/<ins class="diffchange diffchange-inline">flavor</ins>"><ins class="diffchange diffchange-inline">flavor</ins></a> <ins class="diffchange diffchange-inline">elements, </ins><a href="https://2010.igem.org/Team:Harvard/<ins class="diffchange diffchange-inline">allergy</ins>"><ins class="diffchange diffchange-inline">allergen</ins></a> <ins class="diffchange diffchange-inline">knockdown</ins>, <a href="https://2010.igem.org/Team:Harvard/<ins class="diffchange diffchange-inline">color</ins>"><ins class="diffchange diffchange-inline">pigment</ins></a> <ins class="diffchange diffchange-inline">accumulation</ins>, <ins class="diffchange diffchange-inline">or </ins><a href="https://2010.igem.org/Team:Harvard/<ins class="diffchange diffchange-inline">fences</ins>"><ins class="diffchange diffchange-inline">fence</ins></a> <ins class="diffchange diffchange-inline">mechanisms. We next built a set of </ins><a href="https://2010.igem.org/Team:Harvard/<ins class="diffchange diffchange-inline">vectors/vectors</ins>"><ins class="diffchange diffchange-inline">plant vectors</ins></a> <ins class="diffchange diffchange-inline">compatible with </ins>the <ins class="diffchange diffchange-inline">BioBrick standard and moved our parts from e. coli </ins>into <ins class="diffchange diffchange-inline">these </ins>vectors, <ins class="diffchange diffchange-inline">which allowed us to electroporate our parts </ins>into <ins class="diffchange diffchange-inline">agrobactera. The final step was to transform arabidopsis flowers with our engineered </ins>agrobacteria <ins class="diffchange diffchange-inline">and select for </ins>plants containing <ins class="diffchange diffchange-inline">our designed constructs</ins>.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2010/0/03/Harvard2010roadmap.png" width="600px"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2010/0/03/Harvard2010roadmap.png" width="600px"></div></td></tr>
</table>Devin