Team:Harvard/vectors

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Revision as of 19:49, 12 July 2010




abstract

Our goal is the creation of an "iGarden" with the biobrick standard and in order for such a concept to work, we must get our constructs into plants. We used agrobacterium-mediated transformation, first in arabidopsis, then in strawberries.

To do so, we created a set of vectors compatible with the biobrick standard. To express our constucts - such as flavor elements, allergen knockdown, or fence mechanisms, we cloned the constucts into the agrobacterium vectors with E. coli as the chassis, then transformed into agrobacteria via electroporation, then innoculated our plants with the contruct containing agrobacteria.