Team:Harvard/vectors

From 2010.igem.org

(Difference between revisions)
Line 1: Line 1:
-
<html>
+
{{harvardmain|banner=http://2010.igem.org/wiki/images/7/7d/Harvard2010vectorbanner.png}}
-
<head>
+
-
<style>
+
-
#allcontent {
+
-
      width: 950px;
+
-
}
+
-
#sidebar a{
+
-
      text-decoration: none;
+
-
      color: #7F5217;
+
-
}
+
-
#sidebar a:hover{
+
-
    color: #A0C544;
+
-
    text-decoration: none;
+
-
}
+
-
body {
+
-
        margin-left: 10%;
+
-
font-family: Helvetica, Arial, sans-serif;
+
-
width: 900px;
+
-
font-size: 0.9 em;
+
-
color: #254117;
+
-
}
+
-
 
+
-
#headerlinks{
+
-
      margin-left: -20px;
+
-
}
+
-
#nav, #nav ul {
+
-
padding: 0;
+
-
list-style: none;
+
-
}
+
-
 
+
-
#nav a {
+
-
display: block;
+
-
width: 149px;
+
-
}
+
-
 
+
-
#nav li {
+
-
float: left;
+
-
width: 149px;
+
-
margin-right: 41px;
+
-
}
+
-
 
+
-
#nav li ul {
+
-
position: absolute;
+
-
width: 149px;
+
-
left: -999em;
+
-
}
+
-
 
+
-
#nav li:hover ul {
+
-
left: auto;
+
-
      text-decoration: none;
+
-
      color: white;
+
-
 
+
-
}
+
-
 
+
-
#nav li:hover ul, #nav li.sfhover ul {
+
-
left: auto;
+
-
      text-decoration: none;
+
-
      color: white;
+
-
}
+
-
 
+
-
sfHover = function() {
+
-
var sfEls = document.getElementById("nav").getElementsByTagName("LI");
+
-
for (var i=0; i<sfEls.length; i++) {
+
-
sfEls[i].onmouseover=function() {
+
-
this.className+=" sfhover";
+
-
}
+
-
sfEls[i].onmouseout=function() {
+
-
this.className=this.className.replace(new RegExp(" sfhover\\b"), "");
+
-
}
+
-
}
+
-
}
+
-
if (window.attachEvent) window.attachEvent("onload", sfHover);
+
-
.bannerimage {
 
-
 
-
}
 
-
 
-
a.bannerlinks {
 
-
  color: white;
 
-
  text-decoration: none;
 
-
  }
 
-
 
-
a.bannerlinks:hover {
 
-
  background-color: #A0C544;
 
-
  color: white;
 
-
  text-decoration: none;
 
-
  }
 
-
 
-
a.bannertoplinks {
 
-
  color: white;
 
-
}
 
-
  a.bannertoplinks:hover {
 
-
  background-color: #A0C544;
 
-
  color: white;
 
-
  text-decoration: none;
 
-
  }
 
-
 
-
.bannerlinks {
 
-
background-color: #7F5217;
 
-
border-style: solid;
 
-
border-color: white;
 
-
border-width: 1px;
 
-
color: white;
 
-
padding: 3px 20px 3px 20px;
 
-
      margin-left: -19px;
 
-
}
 
-
 
-
.bannertoplinks {
 
-
background-color: #827B60;
 
-
border-style: solid;
 
-
border-color: white;
 
-
border-width: 1px;
 
-
color: white;
 
-
        margin-bottom: -2px;
 
-
padding: 3px 20px 3px 20px;
 
-
}
 
-
 
-
#twitter_div {
 
-
font-size: 0.8em;
 
-
      width: 280px;
 
-
}
 
-
 
-
#sidebar {
 
-
width: 300px;
 
-
float: right;
 
-
margin-right: 6px;
 
-
border-style: solid;
 
-
border-color: white;
 
-
border-width: 1px;
 
-
padding: 0px;
 
-
}
 
-
 
-
#abstract {
 
-
width: 600px;
 
-
border-style: solid;
 
-
border-color: white;
 
-
border-width: 1px;
 
-
padding: 10px;
 
-
line-height: 1.2em;
 
-
 
-
}
 
-
 
-
#twitter_div ul {
 
-
line-height: 1.3em;
 
-
text-indent: 3px;
 
-
list-style-image: url('http://2010.igem.org/wiki/images/1/11/Harvard2010leaficon.png');
 
-
}
 
-
 
-
#rightgroup {
 
-
float: right;
 
-
width: 300px;
 
-
padding: 10px;
 
-
}
 
-
 
-
#leftgroup {
 
-
width: 300px;
 
-
padding: 10px;
 
-
        margin-right: 20px;
 
-
}
 
-
 
-
#maincontent {
 
-
width: 610px;
 
-
}
 
-
 
-
a.sidebarlinks {
 
-
display: block;
 
-
width: 300px;
 
-
font-size: 1.2em;
 
-
text-align: center;
 
-
background-color: #827B60;
 
-
color: white;
 
-
padding: 10px 0px 10px 0px;
 
-
margin-bottom: 5px;
 
-
}
 
-
a.sidebarlinks:hover {
 
-
background-color: #A0C544;
 
-
}
 
-
 
-
</style>
 
-
</head>
 
-
<body>
 
-
<img class="bannerimage" src="http://2010.igem.org/wiki/images/7/7d/Harvard2010vectorbanner.png" width="950px"><br />
 
-
<div id="headerlinks">
 
-
<ul id="nav">
 
-
<li>
 
-
<a class="bannertoplinks" href="http://2010.igem.org/Team:Harvard">home</a>
 
-
</li>
 
-
 
-
<li><a class="bannertoplinks" href="#">project</a>
 
-
<ul>
 
-
<li><a class="bannerlinks" href="http://2010.igem.org/Team:Harvard/vectors">vectors</a></li>
 
-
<li><a class="bannerlinks" href="http://2010.igem.org/Team:Harvard/allergy">allergy</a></li>
 
-
<li><a class="bannerlinks" href="http://2010.igem.org/Team:Harvard/flavor">flavor</a></li>
 
-
<li><a class="bannerlinks" href="http://2010.igem.org/Team:Harvard/fences">genetic fence</a></li>
 
-
<li><a class="bannerlinks" href="http://2010.igem.org/Team:Harvard/human_practices/index">human practices</a></li>
 
-
</ul>
 
-
</li>
 
-
<li><a class="bannertoplinks" href="#">people</a>
 
-
<ul>
 
-
<li><a class="bannerlinks" href="http://2010.igem.org/Team:Harvard/students">students</a></li>
 
-
<li><a class="bannerlinks" href="http://2010.igem.org/Team:Harvard/advisors">advisors</a></li>
 
-
<li><a class="bannerlinks" href="http://2010.igem.org/Team:Harvard/sponsors">sponsors</a></li>
 
-
<li><a class="bannerlinks" href="http://2010.igem.org/Team:Harvard/gallery">gallery</a></li>
 
-
</ul>
 
-
</li>
 
-
<li><a class="bannertoplinks" href="#">online</a>
 
-
<ul>
 
-
<li><a class="bannerlinks" href="http://harvardigem.org">blog</a></li>
 
-
<li><a class="bannerlinks" href="http://twitter.com/harvardigem">twitter</a></li>
 
-
<li><a class="bannerlinks" href="http://openwetware.org/wiki/IGEM:Harvard/2010">openwetware</a></li>
 
-
<li><a class="bannerlinks" href="http://www.facebook.com/pages/Cambridge-MA/Harvard-iGEM/269794024286?ref=ts">facebook</a></li>
 
-
</ul>
 
-
</li>
 
-
<li><a class="bannertoplinks" href="http://2010.igem.org/Team:Harvard/contact">contact us</a>
 
-
</li>
 
-
</ul>
 
</div>
</div>
<br /><br />
<br /><br />

Revision as of 15:00, 13 July 2010


</div>

abstract

Our goal is the creation of an "iGarden" with the biobrick standard and in order for such a concept to work, we must get our constructs into plants. We used agrobacterium-mediated transformation, first in arabidopsis, then in strawberries.

To do so, we created a set of <a href="vectors.html">vectors</a> compatible with the biobrick standard. To express our constucts - such as <a href="../flavor/index.html">flavor</a> elements, <a href="../allergy/index.html">allergen</a> knockdown, or <a href="../fence/index.html">fence</a> mechanisms, we cloned the constucts into the agrobacterium vectors with E. coli as the chassis, then transformed into agrobacteria via electroporation, then innoculated our plants with the contruct containing agrobacteria.

<img src="Harvard2010roadmap.png" width="600px">


</body>

</html>