Team:Harvard/vectors

From 2010.igem.org

(Difference between revisions)
Line 169: Line 169:
<ul id="nav">
<ul id="nav">
<li>
<li>
-
<a class="bannertoplinks" href="../index.html">home</a>
+
<a class="bannertoplinks" href="https://2010.igem.org/Team:Harvard">home</a>
</li>
</li>
<li><a class="bannertoplinks" href="#">project</a>
<li><a class="bannertoplinks" href="#">project</a>
<ul>
<ul>
-
<li><a class="bannerlinks" href="#">vectors</a></li>
+
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Harvard/vectors">vectors</a></li>
-
<li><a class="bannerlinks" href="../allergy.html">allergy</a></li>
+
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Harvard/allergy">allergy</a></li>
-
<li><a class="bannerlinks" href="#">flavor</a></li>
+
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Harvard/flavor">flavor</a></li>
-
<li><a class="bannerlinks" href="#">genetic fence</a></li>
+
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Harvard/fences">genetic fence</a></li>
-
<li><a class="bannerlinks" href="#">human practices</a></li>
+
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Harvard/human_practices/index">human practices</a></li>
</ul>
</ul>
</li>
</li>
<li><a class="bannertoplinks" href="#">people</a>
<li><a class="bannertoplinks" href="#">people</a>
<ul>
<ul>
-
<li><a class="bannerlinks" href="#">students</a></li>
+
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Harvard/students">students</a></li>
-
<li><a class="bannerlinks" href="#">advisors</a></li>
+
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Harvard/advisors">advisors</a></li>
-
<li><a class="bannerlinks" href="#">sponsors</a></li>
+
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Harvard/sponsors">sponsors</a></li>
-
<li><a class="bannerlinks" href="#">gallery</a></li>
+
<li><a class="bannerlinks" href="https://2010.igem.org/Team:Harvard/gallery">gallery</a></li>
</ul>
</ul>
</li>
</li>
Line 197: Line 197:
</ul>
</ul>
</li>
</li>
-
<li><a class="bannertoplinks" href="mailto:igemharvard@gmail.com">contact us</a>
+
<li><a class="bannertoplinks" href="https://2010.igem.org/Team:Harvard/contact">contact us</a>
</li>
</li>
-
</ul>
+
</ul>
-
</div>
+
</div>
<br /><br />
<br /><br />

Revision as of 16:23, 12 July 2010




abstract

Our goal is the creation of an "iGarden" with the biobrick standard and in order for such a concept to work, we must get our constructs into plants. We used agrobacterium-mediated transformation, first in arabidopsis, then in strawberries.

To do so, we created a set of vectors compatible with the biobrick standard. To express our constucts - such as flavor elements, allergen knockdown, or fence mechanisms, we cloned the constucts into the agrobacterium vectors with E. coli as the chassis, then transformed into agrobacteria via electroporation, then innoculated our plants with the contruct containing agrobacteria.