Team:Harvard/parts

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parts and primers

NameOriginal NameDescriptionResistanceResistance (Plants)Sequence
V1pORE Open Series 1 (O1)Agrobacterium vector open seriesKanamycinpat pORE O1
V2pORE Open Series 2 (O2)Agrobacterium vector open seriesKanamycinnptII pORE O2
V3pORE Expression Series 3 (E3)Agrobacterium vector with ENTCUP2 promoterKanamycinpat pORE E3
V4pORE Expression Series 4 (E4)Agrobacterium vector with ENTCUP2 promoterKanamycinnptII pORE E4
V5pORE Reporter Series 1 (R1)Agrobacterium vector with gusA reporterKanamycinnptII pORE R1
V6pORE Reporter Series 3 (R3)Agrobacterium vector with smgfp reporterKanamycinnptII pORE R3
V7
-
Agrobacterium vector open series with biobrick cloning siteKanamycinpat V7
V8
-
Agrobacterium vector open series with biobrick cloning siteKanamycinnptII V8
V9
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinpat V9
V10
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinnptII V10
V11
-
Agrobacterium vector with gusA reporter and biobrick cloning siteKanamycinnptII V11
V12
-
Agrobacterium vector with smgfp reporter and biobrick cloning siteKanamycinnptII V12
A1amiRNA GFPGFP knockdown constructchloramphenicol- amirnaGFP
A2amiRNA LTP LTP knockdown constructchloramphenicol- amiRNA LTP
A3ihpRNA BetIntron hairpin Bet Knockdown constructchloramphenicol- ihpRNA Bet1
A4ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicol-ihpRNA LTP
A5ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicol-ihpRNA GER
F1pENTCUP2 promoterPlant specific promoter. Used to drive expression of our flavor constructs in plants. chloramphenicol-
F2NOSt terminatorPlant specific terminator. Terminates transcription but no stop codon on end.chloramphenicol-
F3NOSt terminator + stopPlant specific terminator. Terminates transcription with stop codon on end.chloramphenicol-
F4BrazzeinSweet-tasting protein. 54 amino acid sequence. chloramphenicol-
F5MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencechloramphenicol-
F6Brazzein YFP2x C-terminusBrazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-
F7Miraculin YFP2x C-terminusMiraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-
F8Brazzein StrepII C-terminusBrazzein with StrepII tag on C-terminus for Western Blot.chloramphenicol-
F9Miraculin StrepII C-terminusMiraculin with StrepII tag on C-terminus for Western Blot.chloramphenicol-
C1amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2)knockdownchloramphenicol-
C2amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownchloramphenicol-
C3amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownchloramphenicol-
GF1Barnase Fatal gene for the genetic fence chloramphenicol-
GF2BarstarProtein inhibitor of Barnasechloramphenicol-
GF3LacINLac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoterchloramphenicol-
GF4RXRLc-VP16 Fusion protein of Locust Retinoic Acid Receptor and VP16 activation domain - binds EcR-Gal4 in presence of Methoxyfenozidechloramphenicol-
GF5EcR-Gal4 Fusion protein of Ecdysone receptor and Gal4 DNA binding domain - binds RXRLc-VP16 and initiates transcription downstream of Gal Upstream Activating Sequence in presence of Methoxyfenozide chloramphenicol-