Team:Harvard/parts

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<div id="abstract">
 
<br><br>
<br><br>
<h1>parts</h1>
<h1>parts</h1>
<table cellspacing="0" width="900">
<table cellspacing="0" width="900">
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<tr><td><b>Name</b></td><td><b>Biobrick Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Resistance (Plants)</b></td><td><b>Sequence</b></td></tr>
 
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<tr><td>V1</td><td>BBa_K382000</td><td>pORE Open Series 1 (O1)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/37/PORE_O1.gb"> pORE O1 </a></td></tr>
 
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<tr><td>V2</td><td>BBa_K382001</td><td>pORE Open Series 2 (O2)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/f/fc/PORE_O2.gb"> pORE O2 </a></td></tr>
 
-
<tr><td>V3</td><td>BBa_K382002</td><td>pORE Expression Series 3 (E3)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/e/e7/PORE_E3.gb"> pORE E3 </a></td></tr>
 
-
<tr><td>V4</td><td>BBa_K382003</td><td>pORE Expression Series 4 (E4)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/b/b9/PORE_E4.gb"> pORE E4 </a></td></tr>
 
-
<tr><td>V5</td><td>BBa_K382004</td><td>pORE Reporter Series 1 (R1)</td><td>Agrobacterium vector with gusA reporter</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/f/fe/PORE_R1.gb"> pORE R1 </a></td></tr>
 
-
<tr><td>V6</td><td>BBa_K382005</td><td>pORE Reporter Series 3 (R3)</td><td>Agrobacterium vector with smgfp reporter</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/e/e8/PORE_R3.gb"> pORE R3 </a></td></tr>
 
-
<tr><td>V7</td><td>BBa_K382006</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb"> V7 </a></td></tr>
 
-
<tr><td>V8</td><td>BBa_K382007</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb"> V8 </a></td></tr>
 
-
<tr><td>V9</td><td>BBa_K382008</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb"> V9 </a></td></tr>
 
-
<tr><td>V10</td><td>BBa_K382009</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb"> V10 </a></td></tr>
 
-
<tr><td>V11</td><td>BBa_K382010</td><td><center>-</center></td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb"> V11 </a></td></tr>
 
-
<tr><td>V12</td><td>BBa_K382011</td><td><center>-</center></td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12 </a></td></tr>
 
-
<tr><td>A1</td><td>BBa_K382012</td><td>amiRNA GFP</td><td>GFP knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/wiki/images/c/cb/AmirnaGFP.txt"> amiRNA GFP</a></td></tr>
+
<tr><td><b>notebook notation</b></td><td><b>biobrick name</b></td><td><b>original name</b></td><td><b>description</b></td><td><b>resistance (bacteria)</b></td><td><b>resistance (plants)</b></td><td><b>sequence</b></td></tr>
-
<tr><td>A2</td><td>BBa_K382013</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>chloramphenicol</td><td>-</td><td> <a href="LTP.txt">amiRNA LTP</a></td></tr>
+
 
-
<tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/wiki/images/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr>
+
<tr><td>V7</td><td>BBa_K382000</td><td>pORE Open Series Vector with BioBrick Sites</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>kan</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb">link</a></td></tr>
-
<tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/wiki/images/6/6f/LTP.txt">ihpRNA LTP</a></td></tr>
+
 
-
<tr><td>A5</td><td>BBa_K382016</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/wiki/images/8/82/Ger.txt">ihpRNA GER</a></td></tr>
+
<tr><td>V8</td><td>BBa_K382001</td><td>pORE Open Series Vector with BioBrick Sites</td><td>Agrobacterium vector open series with biobrick cloning site</td><td>kan</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb">link</a></td></tr>
 +
 
 +
<tr><td>V9</td><td>BBa_K382002</td><td>pORE Expression Series Vector with BioBrick Sites</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>kan</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb">link</a></td></tr>
 +
 
 +
<tr><td>V10</td><td>BBa_K382003</td><td>pORE Expression Series Vector with BioBrick Sites</td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>kan</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb">link</a></td></tr>
 +
 
 +
<tr><td>V11</td><td>BBa_K382004</td><td>pORE Reporter Series Vector with BioBrick Sites</td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>kan</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb">link</a></td></tr>
 +
 
 +
<tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>kan</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb">link</a></td></tr>
 +
 
 +
<tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/wiki/images/b/b9/Lut2.txt">link</a></td></tr>
 +
 
 +
<tr><td>C2</td><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/wiki/images/d/da/Beta-ophase.txt">link</a></td></tr>
 +
 
 +
<tr><td>C3</td><td>BBa_K382012</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/wiki/images/0/0f/LYC.txt">link</a></td></tr>
 +
 
 +
<tr><td>A2</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>amp</td><td>-</td><td><a href="http://2010.igem.org/wiki/images/e/ee/Bet1.txt">link</a></td></tr>
 +
 
 +
<tr><td>A3</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/wiki/images/6/6f/LTP.txt">link</a></td></tr>
 +
 
 +
<tr><td></td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>amp</td><td>-</td><td> <a href="http://2010.igem.org/Team:Harvard/allergy/LTP.txt">link</a></td></tr>
 +
 
 +
<tr><td></td><td>BBa_K382018</td><td>PDK intron hairpin</td><td>Intron hairpin construct</td><td>amp</td><td>-</td><td><a href="http://partsregistry.org/Part:BBa_K382018">link</a></td></tr>
 +
 
 +
<tr><td>A6</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/wiki/images/8/82/Ger.txt">link</a></td></tr>
 +
 
 +
<tr><td>F1</td><td>BBa_K382020</td><td><i>Arabidopsis</i>-optimized Brazzein</td><td>Sweet-tasting protein.  54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr>
 +
 +
<tr><td>F2</td><td>BBa_K382021</td><td><i>Arabidopsis</i>-optimized Miraculin</td><td>Taste-inverting protein turns sour to sweet.  191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr>
 +
 
 +
<tr><td>F3</td><td>BBa_K382022</td><td>pENTCUP2 promoter</td><td>Plant specific promoter.  Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr>
 +
 
 +
<tr><td>F4</td><td>BBa_K382023</td><td>NOSt terminator</td><td>Plant specific terminator.  Terminates transcription but no stop codon on end.</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr>
 +
 
 +
<tr><td>F5</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator.  Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr>
 +
 
 +
<tr><td>F6</td><td>BBa_K382025</td><td><i>Arabidopsis</i>-optimized Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on N-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr>
 +
 
 +
<tr><td>F7</td><td>BBa_K382026</td><td><i>Arabidopsis</i>-optimized Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on N-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr>
 +
 
 +
<tr><td>F8</td><td>BBa_K382027</td><td><i>Arabidopsis</i>-optimized Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr>
 +
 
 +
<tr><td>F9</td><td>BBa_K382028</td><td><i>Arabidopsis</i>-optimized Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr>
 +
 
 +
<tr><td>E2</td><td>BBa_K382030</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/f5">link</a></td></tr>
 +
 
 +
<tr><td>E3</td><td>BBa_K382031</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>amp</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/f4">link</a></td></tr>
 +
 +
<tr><td>E5</td><td>BBa_K382033</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/f7">link</a></td></tr>
 +
 
 +
<tr><td>E6</td><td>BBa_K382034</td><td>Act2pLacO</td><td>Actin promoter with LacO sites </td><td>amp</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/f12">link</a></td></tr>
 +
 
 +
<tr><td></td><td>BBa_K382035</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/f3">link</a></td></tr>
 +
 
 +
<tr><td></td><td>BBa_K382036</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>amp</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/f5">link</a></td></tr>
 +
 
 +
<tr><td></td><td>BBa_K382037</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>amp</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/f7">link</a></td></tr>
 +
 
 +
<tr><td></td><td>BBa_K382040</td><td><i>Arabidopsis</i>-optimized Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/F7">link</a></td></tr>
 +
 
 +
<tr><td></td><td>BBa_K382041</td><td><i>Arabidopsis</i>-optimized Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus.  Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/F6">link</a></td></tr>
 +
 
 +
<tr><td>V21</td><td>BBa_K382050</td><td><i>Arabidopsis</i>-optimized Miraculin</td><td>Taste-inverting protein turns sour to sweet.  191 amino acid sequence</td><td>kan</td><td>pat</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr>
 +
 
 +
<tr><td>V22</td><td>BBa_K382051</td><td><i>Arabidopsis</i>-optimized Miraculin</td><td>Taste-inverting protein turns sour to sweet.  191 amino acid sequence</td><td>kan</td><td>nptII</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr>
 +
 
 +
<tr><td>V25</td><td>BBa_K382052</td><td><i>Arabidopsis</i>-optimized Brazzein</td><td>Sweet-tasting protein.  54 amino acid sequence. </td><td>kan</td><td>pat</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr>
 +
 
 +
<tr><td>V26</td><td>BBa_K382053</td><td><i>Arabidopsis</i>-optimized Brazzein</td><td>Sweet-tasting protein.  54 amino acid sequence. </td><td>kan</td><td>nptII</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr>
 +
 
 +
<tr><td>V29</td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan</td><td>pat</td><td><a href="http://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr>
 +
 
 +
<tr><td>V30</td><td>BBa_K382055</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan</td><td>nptII</td><td><a href="http://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr>
 +
 
 +
<tr><td>V33</td><td>BBa_K382058</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan</td><td>pat</td><td><a href="http://2010.igem.org/wiki/images/b/b9/Lut2.txt">link</a></td></tr>
 +
 
 +
<tr><td>V35</td><td>BBa_K382059</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan</td><td>pat</td><td><a href="http://2010.igem.org/wiki/images/d/da/Beta-ophase.txt">link</a></td></tr>
 +
 
 +
<tr><td>V37</td><td>BBa_K382060</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan</td><td>pat</td><td><a href="http://2010.igem.org/wiki/images/0/0f/LYC.txt">link</a></td></tr>
 +
 
 +
<tr><td>V34</td><td>BBa_K382061</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>kan</td><td>nptII</td><td><a href="http://2010.igem.org/wiki/images/b/b9/Lut2.txt">link</a></td></tr>
 +
 
 +
<tr><td>V36</td><td>BBa_K382062</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>kan</td><td>nptII</td><td><a href="http://2010.igem.org/wiki/images/d/da/Beta-ophase.txt">link</a></td></tr>
 +
 
 +
<tr><td>V38</td><td>BBa_K382063</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>kan</td><td>nptII</td><td><a href="http://2010.igem.org/wiki/images/0/0f/LYC.txt">link</a></td></tr>
 +
 
 +
<tr><td></td><td>BBa_K382064</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan</td><td>pat</td><td> <a href="http://2010.igem.org/Team:Harvard/allergy/LTP.txt">link</a></td></tr>
 +
 
 +
<tr><td></td><td>BBa_K382065</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>kan</td><td>nptII</td><td> <a href="http://2010.igem.org/Team:Harvard/allergy/LTP.txt">link</a></td></tr>
 +
 
 +
<tr><td></td><td>BBa_K382066</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan</td><td>pat</td><td><a href="http://2010.igem.org/wiki/images/8/82/Ger.txt">link</a></td></tr>
 +
 
 +
<tr><td></td><td>BBa_K382067</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>kan</td><td>nptII</td><td><a href="http://2010.igem.org/wiki/images/8/82/Ger.txt">link</a></td></tr>
-
<tr><td>F1</td><td>BBa_K382017</td><td>pENTCUP2 promoter</td><td>Plant specific promoter.  Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr>
+
<tr><td></td><td>BBa_K382068</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan</td><td>pat</td><td><a href="http://2010.igem.org/wiki/images/e/ee/Bet1.txt">link</a></td></tr>
-
<tr><td>F2</td><td>BBa_K382018</td><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382023">link</a></td></tr>
+
-
<tr><td>F3</td><td>BBa_K382019</td><td>NOSt terminator + stop</td><td>Plant specific terminator.  Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr>
+
-
<tr><td>F4</td><td>BBa_K382020</td><td>Brazzein</td><td>Sweet-tasting protein.  54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr>
+
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<tr><td>F5</td><td>BBa_K382021</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet.  191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr>
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<tr><td>F6</td><td>BBa_K382022</td><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus.  Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/F6">link</a></td></tr>
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<tr><td>F7</td><td>BBa_K382023</td><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td></td></tr>
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<tr><td>F8</td><td>BBa_K382024</td><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr>
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<tr><td>F9</td><td>BBa_K382025</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr>
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<tr><td></td><td>BBa_K382069</td><td>ihpRNA BetV1</td><td>Intron hairpin Bet Knockdown construct</td><td>kan</td><td>nptII</td><td><a href="http://2010.igem.org/wiki/images/e/ee/Bet1.txt">link</a></td></tr>
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<tr><td>C1</td><td>BBa_K382026</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/wiki/images/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr>
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<tr><td></td><td>BBa_K382070</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan</td><td>pat</td><td><a href="http://2010.igem.org/wiki/images/6/6f/LTP.txt">link</a></td></tr>
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<tr><td>C2</td><td>BBa_K382027</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/wiki/images/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr>
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<tr><td>C3</td><td>BBa_K382028</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/wiki/images/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr>
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<tr><td>GF1</td><td>BBa_K382029</td><td>Barnase</td><td> Fatal gene for the genetic fence </td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/f2">link</a></td></tr>
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<tr><td></td><td>BBa_K382071</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>kan</td><td>nptII</td><td><a href="http://2010.igem.org/wiki/images/6/6f/LTP.txt">link</a></td></tr>
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<tr><td>GF2</td><td>BBa_K382030</td><td>Barstar</td><td>Protein inhibitor of Barnase</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/f2">link</a></td></tr>
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<tr><td>GF3</td><td>BBa_K382031</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/f3">link</a></td></tr>
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<tr><td>GF4</td><td>BBa_K38202</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/f4">link</a></td></tr>
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<tr><td>GF5</td><td>BBa_K382033</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/f5">link</a></td></tr>
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<tr><td>GF6</td><td>BBa_K382034</td><td>VP16</td><td> Part of the Retinoic Acid Receptor and VP16 activation domain fusion protein. VP16 recruits transcription factors, and when colocalized with the Gal4 Upstream Activating Sequence initiates transcription downstream of the 5xGal435s promoter.</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/f6">link</a></td></tr>
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<tr><tr><td>GF7</td><td>BBa_K382035</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/f7">link</a></td></tr>
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<tr><td>GF8</td><td>BBa_K382036</td><td>Gal4 DNA binding domain</td><td> Part of the Gal4-EcR fusion protein. Gal4 associates with the Gal4 upstream activating sequence, and when RXR-VP16 is bound, it localizes VP16 to the Gal4UAS35s promoter resulting in transcription downstream. </td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/f8">link</a></td></tr>
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<tr><td>GF9</td><td>BBa_K382036</td><td>5xGal UAS</td><td> 5 time repeat of Gal4 upstream activating sequence</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/f11">link</a></td></tr>
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        <tr><td>GF10</td><td>BBa_K382037</td><td>Act2lacO</td><td>Actin2 promoter with Lac Inhibitor responsive sites (Lac O sites</td><td>chloramphenicol</td><td>-</td><td><a href="http://2010.igem.org/Team:Harvard/f12">link</a></td></tr>
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</table>
</table>

Latest revision as of 18:06, 27 October 2010




parts

notebook notationbiobrick nameoriginal namedescriptionresistance (bacteria)resistance (plants)sequence
V7BBa_K382000pORE Open Series Vector with BioBrick SitesAgrobacterium vector open series with biobrick cloning sitekanpatlink
V8BBa_K382001pORE Open Series Vector with BioBrick SitesAgrobacterium vector open series with biobrick cloning sitekannptIIlink
V9BBa_K382002pORE Expression Series Vector with BioBrick SitesAgrobacterium vector with ENTCUP2 promoter and biobrick cloning sitekanpatlink
V10BBa_K382003pORE Expression Series Vector with BioBrick SitesAgrobacterium vector with ENTCUP2 promoter and biobrick cloning sitekannptIIlink
V11BBa_K382004pORE Reporter Series Vector with BioBrick SitesAgrobacterium vector with gusA reporter and biobrick cloning sitekannptIIlink
V12BBa_K382005pORE Reporter Series Vector with BioBrick SitesAgrobacterium vector with smgfp reporter and biobrick cloning sitekannptIIlink
C1BBa_K382010amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2) knockdownchloramphenicol-link
C2BBa_K382011amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownchloramphenicol-link
C3BBa_K382012amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownchloramphenicol-link
A2BBa_K382014ihpRNA BetIntron hairpin Bet Knockdown constructamp-link
A3BBa_K382015ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicol-link
BBa_K382017amiRNA LTP LTP knockdown constructamp- link
BBa_K382018PDK intron hairpinIntron hairpin constructamp-link
A6BBa_K382019ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicol-link
F1BBa_K382020Arabidopsis-optimized BrazzeinSweet-tasting protein. 54 amino acid sequence. chloramphenicol-link
F2BBa_K382021Arabidopsis-optimized MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencechloramphenicol-link
F3BBa_K382022pENTCUP2 promoterPlant specific promoter. Used to drive expression of our flavor constructs in plants. chloramphenicol-link
F4BBa_K382023NOSt terminatorPlant specific terminator. Terminates transcription but no stop codon on end.chloramphenicol-link
F5BBa_K382024NOSt terminator + stopPlant specific terminator. Terminates transcription with stop codon on 5' end.chloramphenicol-link
F6BBa_K382025Arabidopsis-optimized Miraculin StrepII N-terminusMiraculin with StrepII tag on N-terminus for Western Blot.chloramphenicol-link
F7BBa_K382026Arabidopsis-optimized Brazzein StrepII N-terminusBrazzein with StrepII tag on N-terminus for Western Blot.chloramphenicol-link
F8BBa_K382027Arabidopsis-optimized Miraculin StrepII C-terminusMiraculin with StrepII tag on C-terminus for Western Blot.chloramphenicol-link
F9BBa_K382028Arabidopsis-optimized Brazzein StrepII C-terminusBrazzein with StrepII tag on C-terminus for Western Blot.chloramphenicol-link
E2BBa_K382030RXRHmHuman Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.chloramphenicol-link
E3BBa_K382031RXRLc Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozideamp-link
E5BBa_K382033Ecdysone Receptor Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide chloramphenicol-link
E6BBa_K382034Act2pLacOActin promoter with LacO sites amp-link
BBa_K382035LacINLac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoterchloramphenicol-link
BBa_K382036RXRHmHuman Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.amp-link
BBa_K382037Ecdysone Receptor Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide amp-link
BBa_K382040Arabidopsis-optimized Miraculin YFP2x C-terminusMiraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-link
BBa_K382041Arabidopsis-optimized Brazzein YFP2x C-terminusBrazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-link
V21BBa_K382050Arabidopsis-optimized MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencekanpatlink
V22BBa_K382051Arabidopsis-optimized MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencekannptIIlink
V25BBa_K382052Arabidopsis-optimized BrazzeinSweet-tasting protein. 54 amino acid sequence. kanpatlink
V26BBa_K382053Arabidopsis-optimized BrazzeinSweet-tasting protein. 54 amino acid sequence. kannptIIlink
V29BBa_K382054amiRNA GFPamiRNA construct for GFP knockdownkanpatlink
V30BBa_K382055amiRNA GFPamiRNA construct for GFP knockdownkannptIIlink
V33BBa_K382058amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2) knockdownkanpatlink
V35BBa_K382059amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownkanpatlink
V37BBa_K382060amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownkanpatlink
V34BBa_K382061amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2) knockdownkannptIIlink
V36BBa_K382062amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownkannptIIlink
V38BBa_K382063amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownkannptIIlink
BBa_K382064amiRNA LTP LTP knockdown constructkanpat link
BBa_K382065amiRNA LTP LTP knockdown constructkannptII link
BBa_K382066ihpRNA Ger Intron hairpin Ger Knockdown constructkanpatlink
BBa_K382067ihpRNA Ger Intron hairpin Ger Knockdown constructkannptIIlink
BBa_K382068ihpRNA BetV1Intron hairpin Bet Knockdown constructkanpatlink
BBa_K382069ihpRNA BetV1Intron hairpin Bet Knockdown constructkannptIIlink
BBa_K382070ihpRNA LTPIntron hairpin LTP Knockdown constructkanpatlink
BBa_K382071ihpRNA LTPIntron hairpin LTP Knockdown constructkannptIIlink