Team:Harvard/parts

From 2010.igem.org

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<tr><td>V11</td><td>BBa_K382004</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb"> V11 </a></td></tr>
<tr><td>V11</td><td>BBa_K382004</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb"> V11 </a></td></tr>
<tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12 </a></td></tr>
<tr><td>V12</td><td>BBa_K382005</td><td>pORE Reporter Series Vector with BioBrick Sites (kan/nptII)</td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12 </a></td></tr>
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<tr><td>A1</td><td>BBa_K382012</td><td>amiRNA GFP</td><td>GFP knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/c/cb/AmirnaGFP.txt"> amiRNA GFP</a></td></tr>
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<tr><td>C1</td><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr>
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<tr><td>C2</td><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr>
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<tr><td>C3</td><td>BBa_K382012</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr>
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<tr><td>A2</td><td>BBa_K382013</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>chloramphenicol</td><td>-</td><td> <a href="LTP.txt">amiRNA LTP</a></td></tr>
<tr><td>A2</td><td>BBa_K382013</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>chloramphenicol</td><td>-</td><td> <a href="LTP.txt">amiRNA LTP</a></td></tr>
<tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr>
<tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/e/ee/Bet1.txt"> ihpRNA Bet1</a></td></tr>
<tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr>
<tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/6/6f/LTP.txt">ihpRNA LTP</a></td></tr>
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<tr><td>A5</td><td>BBa_K382016</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr>
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<tr><td>A4</td><td>BBa_K382018</td><td>PDK intron hairpin</td><td>Intron hairpin construct</td><td>ampicillin</td><td>-</td><td><a href=</a></td></tr>
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<tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">ihpRNA GER</a></td></tr>
<tr><td>F1</td><td>BBa_K382017</td><td>pENTCUP2 promoter</td><td>Plant specific promoter.  Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr>
<tr><td>F1</td><td>BBa_K382017</td><td>pENTCUP2 promoter</td><td>Plant specific promoter.  Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr>
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<tr><td>C1</td><td>BBa_K382026</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/2/24/BBa_K382026_C1.txt">amiRNA LUT2</a></td></tr>
 
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<tr><td>C2</td><td>BBa_K382027</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f2/BBa_K382027_C2.txt">amiRNA BETA-OHASE 1</a></td></tr>
 
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<tr><td>C3</td><td>BBa_K382028</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr>
 
<tr><td>GF1</td><td>BBa_K382029</td><td>Barnase</td><td> Fatal gene for the genetic fence </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f2">link</a></td></tr>
<tr><td>GF1</td><td>BBa_K382029</td><td>Barnase</td><td> Fatal gene for the genetic fence </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f2">link</a></td></tr>

Revision as of 23:07, 26 October 2010



parts

namebiobrick nameoriginal namedescriptionresistance (bacteria)resistance (plants)sequence
V7BBa_K382000pORE Open Series Vector with BioBrick Sites (kan/pat)Agrobacterium vector open series with biobrick cloning siteKanamycinpat V7
V8BBa_K382001pORE Open Series Vector with BioBrick Sites (kan/nptII)Agrobacterium vector open series with biobrick cloning siteKanamycinnptII V8
V9BBa_K382002pORE Expression Series Vector with BioBrick Sites (kan/pat)Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinpat V9
V10BBa_K382003pORE Expression Series Vector with BioBrick Sites (kan/nptII)Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinnptII V10
V11BBa_K382004pORE Reporter Series Vector with BioBrick Sites (kan/nptII)Agrobacterium vector with gusA reporter and biobrick cloning siteKanamycinnptII V11
V12BBa_K382005pORE Reporter Series Vector with BioBrick Sites (kan/nptII)Agrobacterium vector with smgfp reporter and biobrick cloning siteKanamycinnptII V12
C1BBa_K382010amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2) knockdownchloramphenicol-amiRNA LUT2
C2BBa_K382011amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownchloramphenicol-amiRNA BETA-OHASE 1
C3BBa_K382012amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownchloramphenicol-amiRNA LYC
A2BBa_K382013amiRNA LTP LTP knockdown constructchloramphenicol- amiRNA LTP
A3BBa_K382014ihpRNA BetIntron hairpin Bet Knockdown constructchloramphenicol- ihpRNA Bet1
A4BBa_K382015ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicol-ihpRNA LTP
A4BBa_K382018PDK intron hairpinIntron hairpin constructampicillin-
A5BBa_K382019ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicol-ihpRNA GER
F1BBa_K382017pENTCUP2 promoterPlant specific promoter. Used to drive expression of our flavor constructs in plants. chloramphenicol-link
F2BBa_K382018NOSt terminatorPlant specific terminator. Terminates transcription but no stop codon on end.chloramphenicol-link
F3BBa_K382019NOSt terminator + stopPlant specific terminator. Terminates transcription with stop codon on 5' end.chloramphenicol-link
F4BBa_K382020BrazzeinSweet-tasting protein. 54 amino acid sequence. chloramphenicol-link
F5BBa_K382021MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencechloramphenicol-link
F6BBa_K382022Brazzein YFP2x C-terminusBrazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-link
F7BBa_K382023Miraculin YFP2x C-terminusMiraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-link
F8BBa_K382024Brazzein StrepII C-terminusBrazzein with StrepII tag on C-terminus for Western Blot.chloramphenicol-link
F9BBa_K382025Miraculin StrepII C-terminusMiraculin with StrepII tag on C-terminus for Western Blot.chloramphenicol-link
GF1BBa_K382029Barnase Fatal gene for the genetic fence chloramphenicol-link
GF2BBa_K382030BarstarProtein inhibitor of Barnasechloramphenicol-link
GF3BBa_K382031LacINLac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoterchloramphenicol-link
GF4BBa_K38202RXRLc Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozidechloramphenicol-link
GF5BBa_K382033RXRHmHuman Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.chloramphenicol-link
GF6BBa_K382034VP16 Part of the Retinoic Acid Receptor and VP16 activation domain fusion protein. VP16 recruits transcription factors, and when colocalized with the Gal4 Upstream Activating Sequence initiates transcription downstream of the 5xGal435s promoter.chloramphenicol-link
GF7BBa_K382035Ecdysone Receptor Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide chloramphenicol-link
GF8BBa_K382036Gal4 DNA binding domain Part of the Gal4-EcR fusion protein. Gal4 associates with the Gal4 upstream activating sequence, and when RXR-VP16 is bound, it localizes VP16 to the Gal4UAS35s promoter resulting in transcription downstream. chloramphenicol-link
GF9BBa_K3820365xGal UAS 5 time repeat of Gal4 upstream activating sequencechloramphenicol-link
GF10BBa_K382037Act2lacOActin2 promoter with Lac Inhibitor responsive sites (Lac O siteschloramphenicol-link

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