Team:Harvard/parts

From 2010.igem.org

(Difference between revisions)
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<tr><td><b>Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Resistance (Plants)</b></td><td><b>Sequence</b></td></tr>
<tr><td><b>Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Resistance (Plants)</b></td><td><b>Sequence</b></td></tr>
<tr><td>V1</td><td>pORE Open Series 1 (O1)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/37/PORE_O1.gb"> pORE_O1 </a></td></tr>
<tr><td>V1</td><td>pORE Open Series 1 (O1)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/37/PORE_O1.gb"> pORE_O1 </a></td></tr>
-
<tr><td>V2</td><td>pORE Open Series 2 (O2)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
+
<tr><td>V2</td><td>pORE Open Series 2 (O2)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/f/fc/PORE_O2.gb"> pORE_O2 </a></td></tr>
-
<tr><td>V3</td><td>pORE Expression Series 3 (E3)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>pat</td><td></td></tr>
+
<tr><td>V3</td><td>pORE Expression Series 3 (E3)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/e/e7/PORE_E3.gb"> pORE_E3 </a></td></tr>
-
<tr><td>V4</td><td>pORE Expression Series 4 (E4)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
+
<tr><td>V4</td><td>pORE Expression Series 4 (E4)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/b/b9/PORE_E4.gb"> pORE_E4 </a></td></tr>
-
<tr><td>V5</td><td>pORE Reporter Series 1 (R1)</td><td>Agrobacterium vector with gusA reporter</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
+
<tr><td>V5</td><td>pORE Reporter Series 1 (R1)</td><td>Agrobacterium vector with gusA reporter</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/f/fe/PORE_R1.gb"> pORE_R1 </a></td></tr>
-
<tr><td>V6</td><td>pORE Reporter Series 3 (R3)</td><td>Agrobacterium vector with smgfp reporter</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
+
<tr><td>V6</td><td>pORE Reporter Series 3 (R3)</td><td>Agrobacterium vector with smgfp reporter</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/e/e8/PORE_R3.gb"> pORE_R3 </a></td></tr>
-
<tr><td>V7</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td></td></tr>
+
<tr><td>V7</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb"> V7:pORE_O1 biobricked </a></td></tr>
-
<tr><td>V8</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
+
<tr><td>V8</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb"> V8:pORE_O2 biobricked </a></td></tr>
-
<tr><td>V9</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td></td></tr>
+
<tr><td>V9</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb"> V9:pORE_E3 biobricked </a></td></tr>
-
<tr><td>V10</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
+
<tr><td>V10</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb"> V10:pORE_E4 biobricked </a></td></tr>
-
<tr><td>V11</td><td><center>-</center></td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
+
<tr><td>V11</td><td><center>-</center></td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb"> V11:pORE_R1 biobricked </a></td></tr>
-
<tr><td>V12</td><td><center>-</center></td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
+
<tr><td>V12</td><td><center>-</center></td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb"> V12:pORE_R3 biobricked </a></td></tr>
<tr><td>A1</td><td>amiRNA GFP</td><td>GFP knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="AmirnaGFP.txt"> amirnaGFP</a></td></tr>
<tr><td>A1</td><td>amiRNA GFP</td><td>GFP knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="AmirnaGFP.txt"> amirnaGFP</a></td></tr>

Revision as of 03:33, 24 October 2010

parts and primers

NameOriginal NameDescriptionResistanceResistance (Plants)Sequence
V1pORE Open Series 1 (O1)Agrobacterium vector open seriesKanamycinpat pORE_O1
V2pORE Open Series 2 (O2)Agrobacterium vector open seriesKanamycinnptII pORE_O2
V3pORE Expression Series 3 (E3)Agrobacterium vector with ENTCUP2 promoterKanamycinpat pORE_E3
V4pORE Expression Series 4 (E4)Agrobacterium vector with ENTCUP2 promoterKanamycinnptII pORE_E4
V5pORE Reporter Series 1 (R1)Agrobacterium vector with gusA reporterKanamycinnptII pORE_R1
V6pORE Reporter Series 3 (R3)Agrobacterium vector with smgfp reporterKanamycinnptII pORE_R3
V7
-
Agrobacterium vector open series with biobrick cloning siteKanamycinpat V7:pORE_O1 biobricked
V8
-
Agrobacterium vector open series with biobrick cloning siteKanamycinnptII V8:pORE_O2 biobricked
V9
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinpat V9:pORE_E3 biobricked
V10
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinnptII V10:pORE_E4 biobricked
V11
-
Agrobacterium vector with gusA reporter and biobrick cloning siteKanamycinnptII V11:pORE_R1 biobricked
V12
-
Agrobacterium vector with smgfp reporter and biobrick cloning siteKanamycinnptII V12:pORE_R3 biobricked
A1amiRNA GFPGFP knockdown constructchloramphenicol- amirnaGFP
A2amiRNA LTP LTP knockdown constructchloramphenicol- amiRNA LTP
A3ihpRNA BetIntron hairpin Bet Knockdown constructchloramphenicol- ihpRNA Bet1
A4ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicol-ihpRNA LTP
A5ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicol-ihpRNA GER
F1pENTCUP2 promoterPlant specific promoter. Used to drive expression of our flavor constructs in plants. chloramphenicol-
F2NOSt terminatorPlant specific terminator. Terminates transcription but no stop codon on end.chloramphenicol-
F3NOSt terminator + stopPlant specific terminator. Terminates transcription with stop codon on end.chloramphenicol-
F4BrazzeinSweet-tasting protein. 54 amino acid sequence. chloramphenicol-
F5MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencechloramphenicol-
F6Brazzein YFP2x C-terminusBrazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-
F7Miraculin YFP2x C-terminusMiraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-
F8Brazzein StrepII C-terminusBrazzein with StrepII tag on C-terminus for Western Blot.chloramphenicol-
F9Miraculin StrepII C-terminusMiraculin with StrepII tag on C-terminus for Western Blot.chloramphenicol-
C1amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2)knockdownchloramphenicol-
C2amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownchloramphenicol-
C3amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownchloramphenicol-
GF1Barnase Fatal gene for the genetic fence chloramphenicol-
GF2BarstarProtein inhibitor of Barnasechloramphenicol-
GF3LacINLac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoterchloramphenicol-
GF4RXRLc-VP16 Fusion protein of Locust Retinoic Acid Receptor and VP16 activation domain - binds EcR-Gal4 in presence of Methoxyfenozidechloramphenicol-
GF5EcR-Gal4 Fusion protein of Ecdysone receptor and Gal4 DNA binding domain - binds RXRLc-VP16 and initiates transcription downstream of Gal Upstream Activating Sequence in presence of Methoxyfenozide chloramphenicol-