Team:Harvard/parts

From 2010.igem.org

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<tr><td>A4</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="LTP.txt">ihpRNA LTP</a></td></tr>
<tr><td>A4</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="LTP.txt">ihpRNA LTP</a></td></tr>
<tr><td>A5</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="Ger.txt">ihpRNA GER</a></td></tr>
<tr><td>A5</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="Ger.txt">ihpRNA GER</a></td></tr>
 +
 +
<tr><td>pENTCUP2 promoter</td><td>Plant specific promoter.  Used to drive expression of our flavor constructs in plants. </td><td></td></tr>
 +
<tr><td>NOSt terminator</td><td>Plant specific terminator.  Terminates transcription but no stop codon on end.</td><td></td></tr>
 +
<tr><td>NOSt terminator + stop</td><td>Plant specific terminator.  Terminates transcription with stop codon on end.</td><td></td></tr>
 +
<tr><td>Brazzein</td><td>Sweet-tasting protein.  54 amino acid sequence. </td><td></td></tr>
 +
<tr><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet.  191 amino acid sequence</td><td></td></tr>
 +
<tr><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus.  Allows visualization of expression</td><td></td></tr>
 +
<tr><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td></td></tr>
 +
<tr><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td></td></tr>
 +
<tr><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td></td></tr>
</table>
</table>
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Revision as of 00:27, 19 October 2010

parts and primers

NameOriginal NameDescriptionResistanceResistance (Plants)Sequence
V1pORE Open Series 1 (O1)Agrobacterium vector open seriesKanamycinpat
V2pORE Open Series 2 (O2)Agrobacterium vector open seriesKanamycinnptII
V3pORE Expression Series 3 (E3)Agrobacterium vector with ENTCUP2 promoterKanamycinpat
V4pORE Expression Series 4 (E4)Agrobacterium vector with ENTCUP2 promoterKanamycinnptII
V5pORE Reporter Series 1 (R1)Agrobacterium vector with gusA reporterKanamycinnptII
V6pORE Reporter Series 3 (R3)Agrobacterium vector with smgfp reporterKanamycinnptII
V7
-
Agrobacterium vector open series with biobrick cloning siteKanamycinpat
V8
-
Agrobacterium vector open series with biobrick cloning siteKanamycinnptII
V9
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinpat
V10
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinnptII
V11
-
Agrobacterium vector with gusA reporter and biobrick cloning siteKanamycinnptII
V12
-
Agrobacterium vector with smgfp reporter and biobrick cloning siteKanamycinnptII
A1amiRNA GFPGFP knockdown constructchloramphenicol- amirnaGFP
A2amiRNA LTP LTP knockdown constructchloramphenicol- amiRNA LTP
A3ihpRNA BetIntron hairpin Bet Knockdown constructchloramphenicol- ihpRNA Bet1
A4ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicol-ihpRNA LTP
A5ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicol-ihpRNA GER
pENTCUP2 promoterPlant specific promoter. Used to drive expression of our flavor constructs in plants.
NOSt terminatorPlant specific terminator. Terminates transcription but no stop codon on end.
NOSt terminator + stopPlant specific terminator. Terminates transcription with stop codon on end.
BrazzeinSweet-tasting protein. 54 amino acid sequence.
MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequence
Brazzein YFP2x C-terminusBrazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression
Miraculin YFP2x C-terminusMiraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression
Brazzein StrepII C-terminusBrazzein with StrepII tag on C-terminus for Western Blot.
Miraculin StrepII C-terminusMiraculin with StrepII tag on C-terminus for Western Blot.


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