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Team:Harvard/flavor/results
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<p>Coming Soon!</p> | <p>Coming Soon!</p> | ||
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| + | <h2>Miraculin & Brazzein</h2> | ||
| + | <h3>Confirmation with <i>YFP-2x</i> Tags</h3> | ||
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| + | In order to confirm that the Miraculin and Brazzein are able to be expressed in <i>E. Coli</i> we attached a <i>YFP-2x</i> tag sequence to the termini of both proteins. The proteins were placed under an IPTG-expressible promoter and used spectrophotometry to determine the level of YFP fluorescence against a baseline, untagged protein. Figure 1 shows relative-fluorescence at times post induction. In all circumstances the levels of YFP-fluorescence increased | ||
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Revision as of 02:02, 25 October 2010
Results
Coming Soon!
Miraculin & Brazzein
Confirmation with YFP-2x Tags
In order to confirm that the Miraculin and Brazzein are able to be expressed in E. Coli we attached a YFP-2x tag sequence to the termini of both proteins. The proteins were placed under an IPTG-expressible promoter and used spectrophotometry to determine the level of YFP fluorescence against a baseline, untagged protein. Figure 1 shows relative-fluorescence at times post induction. In all circumstances the levels of YFP-fluorescence increased
