Team:Harvard/flavor/parts

From 2010.igem.org

(Difference between revisions)
Line 36: Line 36:
<tr><td>J45017_F</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td></td></tr>
<tr><td>J45017_F</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td></td></tr>
<tr><td>J45017_R</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>
<tr><td>J45017_R</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>
-
<tr><td>NOSterm_BB_F</td><td>HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert</td><td></td></tr>
+
<tr><td>NOSterm_BB_F</td><td>Plant specific terminator forward primer</td><td></td></tr>
-
<tr><td>NOSterm_BB_R</td><td>smGFP-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>
+
<tr><td>NOSterm_BB_R</td><td>Plant specific terminator reverse primer</td><td></td></tr>
<tr><td>Stop_NOSterm_BB_F</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr>
<tr><td>Stop_NOSterm_BB_F</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr>
<tr><td>pENTCUP2_BB_F</td><td>Plant specific promoter forward primer</td><td></td></tr>
<tr><td>pENTCUP2_BB_F</td><td>Plant specific promoter forward primer</td><td></td></tr>

Revision as of 15:41, 10 October 2010


parts and primers

Primers

NameDescriptionSequence
J45004_FHindIII-pENTCUP2 forward primer for Expression Series insert
J45004_RpENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert
J45017_FHindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert
J45017_RgusA-NheI reverse primer for Reporter Series 1 insert
NOSterm_BB_FPlant specific terminator forward primer
NOSterm_BB_RPlant specific terminator reverse primer
Stop_NOSterm_BB_FBiobrick multiple cloning site for annealing with VP10 to create Open Series Insert
pENTCUP2_BB_FPlant specific promoter forward primer
pENTCUP2_BB_RPlant specific promoter reverse primer