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Team:Harvard/flavor/parts
From 2010.igem.org
(Difference between revisions)
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<h2>Primers</h2> | <h2>Primers</h2> | ||
<table cellspacing="0"> | <table cellspacing="0"> | ||
| - | <tr><td><b>Name</b></td><td><b>Description</b></td>< | + | <tr><td><b>Name</b></td><td><b>Description</b></td><</tr> |
| - | <tr><td>J45004_F</td><td>Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Forward primer | + | <tr><td>J45004_F</td><td>Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Forward primer</td></tr> |
| - | <tr><td>J45004_R</td><td>Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Reverse primer | + | <tr><td>J45004_R</td><td>Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Reverse primer</td></tr> |
| - | <tr><td>J45017_F</td><td>Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Forward primer | + | <tr><td>J45017_F</td><td>Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Forward primer</td></tr> |
| - | <tr><td>J45017_R</td><td>Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Reverse primer | + | <tr><td>J45017_R</td><td>Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Reverse primer</td></tr> |
| - | <tr><td>NOSterm_BB_F</td><td>Plant specific terminator forward primer | + | <tr><td>NOSterm_BB_F</td><td>Plant specific terminator forward primer</td></tr> |
| - | <tr><td>NOSterm_BB_R</td><td>Plant specific terminator reverse primer | + | <tr><td>NOSterm_BB_R</td><td>Plant specific terminator reverse primer</td></tr> |
| - | <tr><td>Stop_NOSterm_BB_F</td><td>NOSt plant specific terminator with stop codon on end | + | <tr><td>Stop_NOSterm_BB_F</td><td>NOSt plant specific terminator with stop codon on end</td></tr> |
| - | <tr><td>pENTCUP2_BB_F</td><td>Plant specific promoter forward primer | + | <tr><td>pENTCUP2_BB_F</td><td>Plant specific promoter forward primer</td></tr> |
| - | <tr><td>pENTCUP2_BB_R</td><td>Plant specific promoter reverse primer | + | <tr><td>pENTCUP2_BB_R</td><td>Plant specific promoter reverse primer</td></tr> |
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<h2>Parts</h2> | <h2>Parts</h2> | ||
<table cellspacing="0"> | <table cellspacing="0"> | ||
| - | <tr><td><b>Name</b></td><td><b>Description | + | <tr><td><b>Name</b></td><td><b>Description</b></td></tr> |
| - | <tr><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. | + | <tr><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td></tr> |
| - | <tr><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end. | + | <tr><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td></tr> |
| - | <tr><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on end. | + | <tr><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on end.</td></tr> |
| - | <tr><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. | + | <tr><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td></tr> |
| - | <tr><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence | + | <tr><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td></tr> |
| - | <tr><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | + | <tr><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td></tr> |
| - | <tr><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | + | <tr><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td></tr> |
| - | <tr><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot. | + | <tr><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td></tr> |
| - | <tr><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot. | + | <tr><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td></tr> |
Revision as of 02:08, 25 October 2010
parts and primers
Primers
| Name | Description | <
| J45004_F | Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Forward primer |
| J45004_R | Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Reverse primer |
| J45017_F | Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Forward primer |
| J45017_R | Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Reverse primer |
| NOSterm_BB_F | Plant specific terminator forward primer |
| NOSterm_BB_R | Plant specific terminator reverse primer |
| Stop_NOSterm_BB_F | NOSt plant specific terminator with stop codon on end |
| pENTCUP2_BB_F | Plant specific promoter forward primer |
| pENTCUP2_BB_R | Plant specific promoter reverse primer |
Parts
| Name | Description |
| pENTCUP2 promoter | Plant specific promoter. Used to drive expression of our flavor constructs in plants. |
| NOSt terminator | Plant specific terminator. Terminates transcription but no stop codon on end. |
| NOSt terminator + stop | Plant specific terminator. Terminates transcription with stop codon on end. |
| Brazzein | Sweet-tasting protein. 54 amino acid sequence. |
| Miraculin | Taste-inverting protein turns sour to sweet. 191 amino acid sequence |
| Brazzein YFP2x C-terminus | Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression |
| Miraculin YFP2x C-terminus | Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression |
| Brazzein StrepII C-terminus | Brazzein with StrepII tag on C-terminus for Western Blot. |
| Miraculin StrepII C-terminus | Miraculin with StrepII tag on C-terminus for Western Blot. |
