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Barnase Growth Control

The fatal gene is the key element of the genetic fence, without which no containment will result. To confirm the ability of barnase to contain plants outside the genetic fence, we characterized an inducible barnase system created by the Berkeley 2008 iGEM team.

This barnase system works by expressing background levels of Barstar with Barnase inducible such that it will overwhelm Barstar when induced. A plate reader assay demonstrated the ability of Barnase to inhibit growth in Bacteria, confirming the results from Berkeley 2008.

Barstar Inhibition of Barnase