Team:Harvard/fences/parts

From 2010.igem.org

(Difference between revisions)
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<tr><td>LCRXR</td><td>INSERT DESCRIPTION HERE</td><td>5'-GAT GAA GCT TGG ATC CCT CGA GCT GC-3'</td></tr>
<tr><td>LCRXR</td><td>INSERT DESCRIPTION HERE</td><td>5'-GAT GAA GCT TGG ATC CCT CGA GCT GC-3'</td></tr>
<tr><td>VP16.Hind.Fwd</td><td>INSERT DESCRIPTION HERE</td><td>5'-AAA AAA GCT TAA AAA AAT GGC TAG CGC CGC CAC CAT G-3'</td></tr>
<tr><td>VP16.Hind.Fwd</td><td>INSERT DESCRIPTION HERE</td><td>5'-AAA AAA GCT TAA AAA AAT GGC TAG CGC CGC CAC CAT G-3'</td></tr>
-
<tr><td>VP16.REV</td><td>5'- CTG CAG CGG CCG CTA CTA GTG CCC TTG GAA TTG ACG AGT AC-3'</td><td></td></tr>
+
<tr><td>VP16.Rev</td><td>INSERT DESCRIPTION HERE</td><td>5'-CTG CAG CGG CCG CTA CTA GTG CCC TTG GAA TTG ACG AGT AC-3'</td></tr>
<tr><td>NLS.serine.BB.Fwd</td><td>INSERT DESCRIPTION HERE</td><td>5'-/5Phos/CTA GAA GTT CTG TTG TTC ATC CTA AGA AGA AGA GAA AGG TTT GAA CTA GTA GCG GCC GCT GCA-3'</td></tr>
<tr><td>NLS.serine.BB.Fwd</td><td>INSERT DESCRIPTION HERE</td><td>5'-/5Phos/CTA GAA GTT CTG TTG TTC ATC CTA AGA AGA AGA GAA AGG TTT GAA CTA GTA GCG GCC GCT GCA-3'</td></tr>
<tr><td>NLS.serine.BB.Rev</td><td>INSERT DESCRIPTION HERE</td><td>5'-/5Phos/GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA ACT T-3'</td></tr>
<tr><td>NLS.serine.BB.Rev</td><td>INSERT DESCRIPTION HERE</td><td>5'-/5Phos/GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA ACT T-3'</td></tr>
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<tr><td>Rev.BB.AtEXP2</td><td>for amplification/extraction of an arabidopsis germination-specific promoter</td><td>5'-AAG GCT GCA GCG GCC GCT ACT GAT ATT TGC TGA TGG GCT AAA G-3'</td></tr>
<tr><td>Rev.BB.AtEXP2</td><td>for amplification/extraction of an arabidopsis germination-specific promoter</td><td>5'-AAG GCT GCA GCG GCC GCT ACT GAT ATT TGC TGA TGG GCT AAA G-3'</td></tr>
<tr><td>Fwd.BB.AtARP2</td><td>for amplification/extraction of the arabidopsis ARP2 promoter</td><td>5'-CCT TTC TAG ACC ATC ACA TAT TTG TAG AAT G-3'</td></tr>
<tr><td>Fwd.BB.AtARP2</td><td>for amplification/extraction of the arabidopsis ARP2 promoter</td><td>5'-CCT TTC TAG ACC ATC ACA TAT TTG TAG AAT G-3'</td></tr>
-
<tr><td>VP16</td><td>5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
+
<tr><td>Rev.BB.AtARP2</td><td>for amplification/extraction of the arabidopsis ARP2 promoter</td><td>5'-AAG GCT GCA GCG GCC GCT ACT AGT CTT CTC CGA TTT CTA TAG AG-3'</td></tr>
-
<tr><td>VP17</td><td>1 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
+
<tr><td>barnase2.BB.fwd</td><td>addition of biobrick sites/ends to barnase gene</td><td>5'-CCT TTC TAG AAT GGT ACC GGT TAT CAA CAC G-3'</td></tr>
-
<tr><td>VP18</td><td>2 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
+
<tr><td>BB.Barnase.Rev</td><td>addition of biobrick sites/ends to barnase gene</td><td>5'-AAG GCT GCA GCG GCC GCT ACT AGT TTA TCT GAT TTT TGT AAA GGT CTG-3'</td></tr>
-
<tr><td>VP19</td><td>3 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
+
<tr><td>NLS.BB.Fwd</td><td>addition of biobrick sites/ends to the arabidopsis NLS</td><td>5'-/5Phos/CTA GAT CTT CTG TTG TTC ATC CAT AGA AGA AGA GAA AGG TTT GAA CTA GTA GCG GCC GCT GCA-3'</td></tr>
<tr><td>VP20</td><td>4 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
<tr><td>VP20</td><td>4 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
<tr><td>VP21</td><td>5 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
<tr><td>VP21</td><td>5 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>

Revision as of 18:20, 3 August 2010


parts and primers

Vectors

NameOriginal NameDescriptionResistanceResistance (Plants)Sequence
V1 TEXT INSERT TEXT HERE Kanamycinpat
V2 TEXT INSERT TEXT HERE KanamycinnptII
V3 TEXT INSERT TEXT HERE Kanamycinpat
V4 TEXT INSERT TEXT HERE KanamycinnptII
V5 TEXT INSERT TEXT HERE KanamycinnptII


Primers

NameDescriptionSequence
Arp2mutForwardPoint mutagenesis to remove restriction enzyme site5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3'
Arp2mutReversePoint mutagenesis to remove restriction enzyme site5'-GAA ATG GAA GAA CTC GTA GAC AAG TCA AGC TCC TTG-3'
Bam.Gal4.FwdINSERT DESCRIPTION HERE5'-AAA AGG ATC CAA AAA AAT GAA GCT ACT GTC TTC TAT C-3'
HmRXR.Hind.RevINSERT DESCRIPTION HERE5'-AAT TAA GCT TGG ATC CCT CGA GCT GCA GC-3'
LCRXRINSERT DESCRIPTION HERE5'-GAT GAA GCT TGG ATC CCT CGA GCT GC-3'
VP16.Hind.FwdINSERT DESCRIPTION HERE5'-AAA AAA GCT TAA AAA AAT GGC TAG CGC CGC CAC CAT G-3'
VP16.RevINSERT DESCRIPTION HERE5'-CTG CAG CGG CCG CTA CTA GTG CCC TTG GAA TTG ACG AGT AC-3'
NLS.serine.BB.FwdINSERT DESCRIPTION HERE5'-/5Phos/CTA GAA GTT CTG TTG TTC ATC CTA AGA AGA AGA GAA AGG TTT GAA CTA GTA GCG GCC GCT GCA-3'
NLS.serine.BB.RevINSERT DESCRIPTION HERE5'-/5Phos/GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA ACT T-3'
Fwd.BB.AtEXP2for amplification/extraction of an arabidopsis germination-specific promoter5'-CCT TTC TAG ACC TGG TGT GGT TTC TTT G-3'
Rev.BB.AtEXP2for amplification/extraction of an arabidopsis germination-specific promoter5'-AAG GCT GCA GCG GCC GCT ACT GAT ATT TGC TGA TGG GCT AAA G-3'
Fwd.BB.AtARP2for amplification/extraction of the arabidopsis ARP2 promoter5'-CCT TTC TAG ACC ATC ACA TAT TTG TAG AAT G-3'
Rev.BB.AtARP2for amplification/extraction of the arabidopsis ARP2 promoter5'-AAG GCT GCA GCG GCC GCT ACT AGT CTT CTC CGA TTT CTA TAG AG-3'
barnase2.BB.fwdaddition of biobrick sites/ends to barnase gene5'-CCT TTC TAG AAT GGT ACC GGT TAT CAA CAC G-3'
BB.Barnase.Revaddition of biobrick sites/ends to barnase gene5'-AAG GCT GCA GCG GCC GCT ACT AGT TTA TCT GAT TTT TGT AAA GGT CTG-3'
NLS.BB.Fwdaddition of biobrick sites/ends to the arabidopsis NLS5'-/5Phos/CTA GAT CTT CTG TTG TTC ATC CAT AGA AGA AGA GAA AGG TTT GAA CTA GTA GCG GCC GCT GCA-3'
VP204 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP215 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP226 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP237 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP248 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP259 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP261 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP272 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP283 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP294 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP305 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP331 of 2 for sequencing V7 and V8 biobrick cloning site
VP342 of 2 for sequencing V7 and V8 biobrick cloning site