Team:Harvard/fences/parts

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<h1>parts and primers</h1>
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<h2>Vectors</h2>
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For more information on the vectors visit our "<a href="vectors.html">meet the vectors</a>" page<br /><br />
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<tr><td><b>Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Resistance (Plants)</b></td><td><b>Sequence</b></td></tr>
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<tr><td>V1</td><td>pORE Open Series 1 (O1)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>pat</td><td></td></tr>
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<tr><td>V2</td><td>pORE Open Series 2 (O2)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V3</td><td>pORE Expression Series 3 (E3)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>pat</td><td></td></tr>
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<tr><td>V4</td><td>pORE Expression Series 4 (E4)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V5</td><td>pORE Reporter Series 1 (R1)</td><td>Agrobacterium vector with gusA reporter</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V6</td><td>pORE Reporter Series 3 (R3)</td><td>Agrobacterium vector with smgfp reporter</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V7</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td></td></tr>
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<tr><td>V8</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V9</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td></td></tr>
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<tr><td>V10</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V11</td><td><center>-</center></td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V12</td><td><center>-</center></td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<h2>Primers</h2>
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<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr>
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<tr><td>VP3</td><td>HindIII-pENTCUP2 forward primer for Expression Series insert</td><td></td></tr>
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<tr><td>VP4</td><td>pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert</td><td></td></tr>
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<tr><td>VP5</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>VP6</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>VP7</td><td>HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>VP8</td><td>smGFP-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>VP9</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr>
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<tr><td>VP11</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr>
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<tr><td>VP12</td><td>1 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>VP13</td><td>2 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>VP14</td><td>3 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>VP15</td><td>4 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>VP16</td><td>5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>VP17</td><td>1 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP18</td><td>2 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP19</td><td>3 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP20</td><td>4 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP21</td><td>5 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP22</td><td>6 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP23</td><td>7 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP24</td><td>8 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP25</td><td>9 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP26</td><td>1 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP27</td><td>2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP28</td><td>3 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP29</td><td>4 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP30</td><td>5 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP33</td><td>1 of 2 for sequencing V7 and V8 biobrick cloning site</td><td></td></tr>
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<tr><td>VP34</td><td>2 of 2 for sequencing V7 and V8 biobrick cloning site</td><td></td></tr>
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Revision as of 14:44, 3 August 2010




parts and primers

Vectors

For more information on the vectors visit our "meet the vectors" page

NameOriginal NameDescriptionResistanceResistance (Plants)Sequence
V1pORE Open Series 1 (O1)Agrobacterium vector open seriesKanamycinpat
V2pORE Open Series 2 (O2)Agrobacterium vector open seriesKanamycinnptII
V3pORE Expression Series 3 (E3)Agrobacterium vector with ENTCUP2 promoterKanamycinpat
V4pORE Expression Series 4 (E4)Agrobacterium vector with ENTCUP2 promoterKanamycinnptII
V5pORE Reporter Series 1 (R1)Agrobacterium vector with gusA reporterKanamycinnptII
V6pORE Reporter Series 3 (R3)Agrobacterium vector with smgfp reporterKanamycinnptII
V7
-
Agrobacterium vector open series with biobrick cloning siteKanamycinpat
V8
-
Agrobacterium vector open series with biobrick cloning siteKanamycinnptII
V9
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinpat
V10
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinnptII
V11
-
Agrobacterium vector with gusA reporter and biobrick cloning siteKanamycinnptII
V12
-
Agrobacterium vector with smgfp reporter and biobrick cloning siteKanamycinnptII


Primers

NameDescriptionSequence
VP3HindIII-pENTCUP2 forward primer for Expression Series insert
VP4pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert
VP5HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert
VP6gusA-NheI reverse primer for Reporter Series 1 insert
VP7HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert
VP8smGFP-NheI reverse primer for Reporter Series 1 insert
VP9Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert
VP11Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert
VP121 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP132 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP143 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP154 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP165 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP171 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP182 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP193 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP204 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP215 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP226 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP237 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP248 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP259 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP261 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP272 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP283 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP294 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP305 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP331 of 2 for sequencing V7 and V8 biobrick cloning site
VP342 of 2 for sequencing V7 and V8 biobrick cloning site