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Plant shRNA

  • Wesley SV, Helliwell CA, Smith NA, Wang MB, Rouse DT, Liu Q, Gooding PS, Singh SP, Abbott D, Stoutjesdijk PA, Robinson SP, Gleave AP, Green AG, and Waterhouse PM. Construct design for efficient, effective and high-throughput gene silencing in plants. Plant J 2001 Sep; 27(6) 581-90. pmid:11576441.
  • Schwab R, Ossowski S, Riester M, Warthmann N, and Weigel D. Highly specific gene silencing by artificial microRNAs in Arabidopsis. Plant Cell 2006 May; 18(5) 1121-33. doi:10.1105/tpc.105.039834 pmid:16531494.

Plant artificial microRNA

Allergen silencing

  • Dodo HW, Konan KN, Chen FC, Egnin M, and Viquez OM. Alleviating peanut allergy using genetic engineering: the silencing of the immunodominant allergen Ara h 2 leads to its significant reduction and a decrease in peanut allergenicity. Plant Biotechnol J 2008 Feb; 6(2) 135-45. doi:10.1111/j.1467-7652.2007.00292.x pmid:17784907.
  • Singh MB and Bhalla PL. Genetic engineering for removing food allergens from plants. Trends Plant Sci 2008 Jun; 13(6) 257-60. doi:10.1016/j.tplants.2008.04.004 pmid:18467156.
  • Le LQ, Mahler V, Lorenz Y, Scheurer S, Biemelt S, Vieths S, and Sonnewald U. Reduced allergenicity of tomato fruits harvested from Lyc e 1-silenced transgenic tomato plants. J Allergy Clin Immunol 2006 Nov; 118(5) 1176-83. doi:10.1016/j.jaci.2006.06.031 pmid:17088146.
  • Gallo M and Sayre R. Removing allergens and reducing toxins from food crops. Curr Opin Biotechnol 2009 Apr; 20(2) 191-6. doi:10.1016/j.copbio.2009.03.005 pmid:19356919.
  • Lorenz Y, Enrique E, Lequynh L, Fötisch K, Retzek M, Biemelt S, Sonnewald U, Vieths S, and Scheurer S. Skin prick tests reveal stable and heritable reduction of allergenic potency of gene-silenced tomato fruits. J Allergy Clin Immunol 2006 Sep; 118(3) 711-8. doi:10.1016/j.jaci.2006.05.014 pmid:16950292.
  • Herman EM, Helm RM, Jung R, and Kinney AJ. Genetic modification removes an immunodominant allergen from soybean. Plant Physiol 2003 May; 132(1) 36-43. doi:10.1104/pp.103.021865 pmid:12746509.
  • Gilissen LJ, Bolhaar ST, Matos CI, Rouwendal GJ, Boone MJ, Krens FA, Zuidmeer L, Van Leeuwen A, Akkerdaas J, Hoffmann-Sommergruber K, Knulst AC, Bosch D, Van de Weg WE, and Van Ree R. Silencing the major apple allergen Mal d 1 by using the RNA interference approach. J Allergy Clin Immunol 2005 Feb; 115(2) 364-9. doi:10.1016/j.jaci.2004.10.014 pmid:15696096.
  • Bhalla PL and Singh MB. Knocking out expression of plant allergen genes. Methods 2004 Mar; 32(3) 340-5. doi:10.1016/j.ymeth.2003.08.011 pmid:14962769.

Allergen proteins

  • Chardin H, Mayer C, Sénéchal H, Wal JM, Poncet P, Desvaux FX, and Peltre G. Lipid transfer protein 1 is a possible allergen in Arabidopsis thaliana. Int Arch Allergy Immunol 2003 Jun; 131(2) 85-90. doi:10.1159/000070923 pmid:12811016.
  • San Miguel-Moncín M, Krail M, Scheurer S, Enrique E, Alonso R, Conti A, Cisteró-Bahíma A, and Vieths S. Lettuce anaphylaxis: identification of a lipid transfer protein as the major allergen. Allergy 2003 Jun; 58(6) 511-7. pmid:12757453.
  • Salcedo G, Sanchez-Monge R, Diaz-Perales A, Garcia-Casado G, and Barber D. Plant non-specific lipid transfer proteins as food and pollen allergens. Clin Exp Allergy 2004 Sep; 34(9) 1336-41. doi:10.1111/j.1365-2222.2004.02018.x pmid:15347364.
  • Gajhede M, Osmark P, Poulsen FM, Ipsen H, Larsen JN, Joost van Neerven RJ, Schou C, Løwenstein H, and Spangfort MD. X-ray and NMR structure of Bet v 1, the origin of birch pollen allergy. Nat Struct Biol 1996 Dec; 3(12) 1040-5. pmid:8946858.
  • Zuidmeer L, Salentijn E, Rivas MF, Mancebo EG, Asero R, Matos CI, Pelgrom KT, Gilissen LJ, and van Ree R. The role of profilin and lipid transfer protein in strawberry allergy in the Mediterranean area. Clin Exp Allergy 2006 May; 36(5) 666-75. doi:10.1111/j.1365-2222.2006.02453.x pmid:16650053.
  • Karlsson AL, Alm R, Ekstrand B, Fjelkner-Modig S, Schiött A, Bengtsson U, Björk L, Hjernø K, Roepstorff P, and Emanuelsson CS. Bet v 1 homologues in strawberry identified as IgE-binding proteins and presumptive allergens. Allergy 2004 Dec; 59(12) 1277-84. doi:10.1111/j.1398-9995.2004.00585.x pmid:15507096.
  • Musidlowska-Persson A, Alm R, and Emanuelsson C. Cloning and sequencing of the Bet v 1-homologous allergen Fra a 1 in strawberry (Fragaria ananassa) shows the presence of an intron and little variability in amino acid sequence. Mol Immunol 2007 Feb; 44(6) 1245-52. doi:10.1016/j.molimm.2006.06.004 pmid:16945416.
  • Breiteneder H and Ebner C. Molecular and biochemical classification of plant-derived food allergens. J Allergy Clin Immunol 2000 Jul; 106(1 Pt 1) 27-36. doi:10.1067/mai.2000.106929 pmid:10887301.
  • Jensen-Jarolim E, Schmid B, Bernier F, Berna A, Kinaciyan T, Focke M, Ebner C, Scheiner O, and Boltz-Nitulescu G. Allergologic exploration of germins and germin-like proteins, a new class of plant allergens. Allergy 2002 Sep; 57(9) 805-10. pmid:12169176.