Team:Harvard/allergy/parts

From 2010.igem.org

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<h1>parts and primers</h1>
<h1>parts and primers</h1>
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<div id="maincontent">
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<div id="abstract">
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<h1>parts and primers</h1>
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<h2>Allergy</h2>
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For more information on the vectors visit our "<a href="update.html">meet the allergens</a>" page<br /><br />
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<table cellspacing="0">
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<tr><td><b>Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Resistance (Plants)</b></td><td><b>Sequence</b></td></tr>
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<tr><td>V1</td><td>amiRNA GFP</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>pat</td><td></td></tr>
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<tr><td>V2</td><td>pORE Open Series 2 (O2)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V3</td><td>pORE Expression Series 3 (E3)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>pat</td><td></td></tr>
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<tr><td>V4</td><td>pORE Expression Series 4 (E4)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V5</td><td>pORE Reporter Series 1 (R1)</td><td>Agrobacterium vector with gusA reporter</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V6</td><td>pORE Reporter Series 3 (R3)</td><td>Agrobacterium vector with smgfp reporter</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V7</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td></td></tr>
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<tr><td>V8</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V9</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td></td></tr>
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<tr><td>V10</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V11</td><td><center>-</center></td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V12</td><td><center>-</center></td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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</table>
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<h2>Primers</h2>
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<table cellspacing="0">
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<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr>
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<tr><td>VP3</td><td>HindIII-pENTCUP2 forward primer for Expression Series insert</td><td></td></tr>
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<tr><td>VP4</td><td>pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert</td><td></td></tr>
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<tr><td>VP5</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>VP6</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>VP7</td><td>HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>VP8</td><td>smGFP-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>VP9</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr>
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<tr><td>VP11</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr>
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<tr><td>VP12</td><td>1 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>VP13</td><td>2 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>VP14</td><td>3 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>VP15</td><td>4 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>VP16</td><td>5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>VP17</td><td>1 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP18</td><td>2 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP19</td><td>3 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP20</td><td>4 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP21</td><td>5 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP22</td><td>6 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP23</td><td>7 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP24</td><td>8 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP25</td><td>9 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP26</td><td>1 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP27</td><td>2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP28</td><td>3 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP29</td><td>4 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP30</td><td>5 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP33</td><td>1 of 2 for sequencing V7 and V8 biobrick cloning site</td><td></td></tr>
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<tr><td>VP34</td><td>2 of 2 for sequencing V7 and V8 biobrick cloning site</td><td></td></tr>
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</table>
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Revision as of 16:03, 10 October 2010

parts and primers

parts and primers

Allergy

For more information on the vectors visit our "meet the allergens" page

NameOriginal NameDescriptionResistanceResistance (Plants)Sequence
V1amiRNA GFPAgrobacterium vector open seriesKanamycinpat
V2pORE Open Series 2 (O2)Agrobacterium vector open seriesKanamycinnptII
V3pORE Expression Series 3 (E3)Agrobacterium vector with ENTCUP2 promoterKanamycinpat
V4pORE Expression Series 4 (E4)Agrobacterium vector with ENTCUP2 promoterKanamycinnptII
V5pORE Reporter Series 1 (R1)Agrobacterium vector with gusA reporterKanamycinnptII
V6pORE Reporter Series 3 (R3)Agrobacterium vector with smgfp reporterKanamycinnptII
V7
-
Agrobacterium vector open series with biobrick cloning siteKanamycinpat
V8
-
Agrobacterium vector open series with biobrick cloning siteKanamycinnptII
V9
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinpat
V10
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinnptII
V11
-
Agrobacterium vector with gusA reporter and biobrick cloning siteKanamycinnptII
V12
-
Agrobacterium vector with smgfp reporter and biobrick cloning siteKanamycinnptII


Primers

NameDescriptionSequence
VP3HindIII-pENTCUP2 forward primer for Expression Series insert
VP4pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert
VP5HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert
VP6gusA-NheI reverse primer for Reporter Series 1 insert
VP7HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert
VP8smGFP-NheI reverse primer for Reporter Series 1 insert
VP9Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert
VP11Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert
VP121 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP132 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP143 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP154 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP165 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP171 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP182 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP193 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP204 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP215 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP226 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP237 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP248 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP259 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP261 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP272 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP283 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP294 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP305 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP331 of 2 for sequencing V7 and V8 biobrick cloning site
VP342 of 2 for sequencing V7 and V8 biobrick cloning site