Team:Harvard/allergy/parts

From 2010.igem.org

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<tr><td>AP3</td><td>3 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> primer3</a></td></tr>
<tr><td>AP3</td><td>3 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> primer3</a></td></tr>
<tr><td>AP4</td><td>4 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> primer4</a></td></tr>
<tr><td>AP4</td><td>4 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> primer4</a></td></tr>
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<tr><td>VP7</td><td>HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>AP5</td><td>1 of 4 primers used to create the amiRNA Bet construct</td><td></td></tr>
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<tr><td>VP8</td><td>smGFP-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>AP6</td><td>2 of 4 primers used to create the amiRNA Bet construct</td><td></td></tr>
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<tr><td>VP9</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr>
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<tr><td>AP7</td><td>3 of 4 primers used to create the amiRNA Bet construct</td><td></td></tr>
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<tr><td>VP11</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr>
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<tr><td>AP8</td><td>4 of 4 primers used to create the amiRNA Bet construct</td><td></td></tr>
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<tr><td>VP12</td><td>1 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>AP9</td><td>Forward primer for Bet v1 Sense</td><td></td></tr>
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<tr><td>VP13</td><td>2 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>AP10</td><td>Reverse primer for Bet v1 Sense </td><td></td></tr>
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<tr><td>VP14</td><td>3 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>AP11</td><td>Forward primer for Bet v1 Antisense</td><td></td></tr>
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<tr><td>VP15</td><td>4 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>AP12</td><td>Reverse primer for Bet v1 Antisense</td><td></td></tr>
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<tr><td>VP16</td><td>5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>AP13</td><td>Forward primer for LTP Sense</td><td></td></tr>
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<tr><td>VP17</td><td>1 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>AP14</td><td>Reverse primer for LTP Sense</td><td></td></tr>
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<tr><td>VP18</td><td>2 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>AP15</td><td>Forward primer for LTP Antisense</td><td></td></tr>
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<tr><td>VP19</td><td>3 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>AP16</td><td>Reverse primer for LTP Antisense</td><td></td></tr>
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<tr><td>VP20</td><td>4 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>AP17</td><td>Forward primer for Ger Sense</td><td></td></tr>
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<tr><td>VP21</td><td>5 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>AP18</td><td>Reverse primer for Ger Sense</td><td></td></tr>
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<tr><td>VP22</td><td>6 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>AP19</td><td>Forward primer for Ger Antisense</td><td></td></tr>
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<tr><td>VP23</td><td>7 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>AP20</td><td>Reverse primer for Ger Antisense</td><td></td></tr>
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<tr><td>VP24</td><td>8 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>AP21</td><td>Forward Primer for PDK intron</td><td></td></tr>
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<tr><td>VP25</td><td>9 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>AP22</td><td>Reverse Primer for PDK intron</td><td></td></tr>
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<tr><td>VP26</td><td>1 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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        <tr><td>AP23</td><td>Forward Primer to make amiRNA Biobrick compatible</td><td></td></tr>
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<tr><td>VP27</td><td>2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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        <tr><td>AP24</td><td>Reverse Primer to make amiRNA Biobrick compatible</td><td></td></tr>
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<tr><td>VP28</td><td>3 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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        <tr><td>AP25</td><td>1 of 4 primers used to create the amiRNA LTP construct</td><td></td></tr>
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<tr><td>VP29</td><td>4 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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        <tr><td>AP26</td><td>2 of 4 primers used to create the amiRNA LTP construct</td><td></td></tr>
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<tr><td>VP30</td><td>5 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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        <tr><td>AP27</td><td>3 of 4 primers used to create the amiRNA LTP construct</td><td></td></tr>
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<tr><td>VP33</td><td>1 of 2 for sequencing V7 and V8 biobrick cloning site</td><td></td></tr>
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        <tr><td>AP28</td><td>4 of 4 primers used to create the amiRNA LTP construct</td><td></td></tr>
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<tr><td>VP34</td><td>2 of 2 for sequencing V7 and V8 biobrick cloning site</td><td></td></tr>
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Revision as of 07:20, 13 October 2010



parts and primers

Allergy

For more information on the allergens visit our "meet the allergens" page

NameOriginal NameDescriptionResistanceSequence
A1amiRNA GFPGFP knockdown constructchloramphenicol amirnaGFP
A2amiRNA (Other allergen x)knockdown construct for x chloramphenicol amiRNA x
A3ihpRNA BetIntron hairpin Bet Knockdown constructchloramphenicol ihpRNA Bet1
A4ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicolihpRNA LTP
A5ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicolihpRNA GER


Primers

NameDescriptionSequence
AP11 of 4 primers used to create the amiRNA GFP construct primer1
AP22 of 4 primers used to create the amiRNA GFP construct primer2
AP33 of 4 primers used to create the amiRNA GFP construct primer3
AP44 of 4 primers used to create the amiRNA GFP construct primer4
AP51 of 4 primers used to create the amiRNA Bet construct
AP62 of 4 primers used to create the amiRNA Bet construct
AP73 of 4 primers used to create the amiRNA Bet construct
AP84 of 4 primers used to create the amiRNA Bet construct
AP9Forward primer for Bet v1 Sense
AP10Reverse primer for Bet v1 Sense
AP11Forward primer for Bet v1 Antisense
AP12Reverse primer for Bet v1 Antisense
AP13Forward primer for LTP Sense
AP14Reverse primer for LTP Sense
AP15Forward primer for LTP Antisense
AP16Reverse primer for LTP Antisense
AP17Forward primer for Ger Sense
AP18Reverse primer for Ger Sense
AP19Forward primer for Ger Antisense
AP20Reverse primer for Ger Antisense
AP21Forward Primer for PDK intron
AP22Reverse Primer for PDK intron
AP23Forward Primer to make amiRNA Biobrick compatible
AP24Reverse Primer to make amiRNA Biobrick compatible
AP251 of 4 primers used to create the amiRNA LTP construct
AP262 of 4 primers used to create the amiRNA LTP construct
AP273 of 4 primers used to create the amiRNA LTP construct
AP284 of 4 primers used to create the amiRNA LTP construct

Retrieved from "http://2010.igem.org/Team:Harvard/allergy/parts"