Team:Harvard/allergy/parts

From 2010.igem.org

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<tr><td><b>Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td></b></td><td><b>Sequence</b></td></tr>
<tr><td><b>Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td></b></td><td><b>Sequence</b></td></tr>
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<tr><td>A1</td><td>amiRNA GFP</td><td>GFP knockdown construct</td><td>chloramphenicol</td><td>&nbsp&nbsp&nbsp"<a href="
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<tr><td>A1</td><td>amiRNA GFP</td><td>GFP knockdown construct</td><td>chloramphenicol</td><td>&nbsp;&nbsp;&nbsp"<a href="
AmirnaGFP.txt"> amiRNA GFP</a>"</td></tr>
AmirnaGFP.txt"> amiRNA GFP</a>"</td></tr>
<tr><td>A2</td><td>amiRNA (Other allergen x)</td><td>knockdown construct for x </td><td>chloramphenicol</td><td> "<a href="insert">amiRNA x</a>"</td></tr>
<tr><td>A2</td><td>amiRNA (Other allergen x)</td><td>knockdown construct for x </td><td>chloramphenicol</td><td> "<a href="insert">amiRNA x</a>"</td></tr>

Revision as of 06:04, 13 October 2010

parts and primers

parts and primers

Allergy

For more information on the vectors visit our "meet the allergens" page

NameOriginal NameDescriptionResistanceSequence
A1amiRNA GFPGFP knockdown constructchloramphenicol  &nbsp" amiRNA GFP"
A2amiRNA (Other allergen x)knockdown construct for x chloramphenicol "amiRNA x"
A3ihpRNA BetIntron hairpin Bet Knockdown constructchloramphenicol" ihpRNA Bet1"
A4ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicol"ihpRNA LTP"
A5ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicol"ihpRNA GER"


Primers

NameDescriptionSequence
VP3HindIII-pENTCUP2 forward primer for Expression Series insert
VP4pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert
VP5HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert
VP6gusA-NheI reverse primer for Reporter Series 1 insert
VP7HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert
VP8smGFP-NheI reverse primer for Reporter Series 1 insert
VP9Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert
VP11Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert
VP121 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP132 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP143 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP154 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP165 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP171 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP182 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP193 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP204 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP215 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP226 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP237 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP248 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP259 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP261 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP272 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP283 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP294 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP305 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP331 of 2 for sequencing V7 and V8 biobrick cloning site
VP342 of 2 for sequencing V7 and V8 biobrick cloning site