Team:Harvard/allergy/parts

From 2010.igem.org

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<div id="abstract">
<div id="abstract">
<h2>Allergy</h2>
<h2>Allergy</h2>
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For more information on the allergens visit our "<a href="allergens">meet the allergens</a>" page<br /><br />
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For more information on the allergens visit our "<a href="allergens">allergen targets</a>" page<br /><br />
<table cellspacing="0">
<table cellspacing="0">
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<tr><td><b>Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Sequence</b></td></tr>
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<tr><td><b>Name</b></td><td><b>Biobrick Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Sequence</b></td></tr>
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<tr><td>A1</td><td>amiRNA GFP</td><td>GFP knockdown construct</td><td>chloramphenicol</td><td><a href="AmirnaGFP.txt"> amirnaGFP</a></td></tr>
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<tr><td>A1</td><td><center>-</center></td><td>amiRNA GFP</td><td>GFP knockdown construct</td><td>chloramphenicol</td><td><a href="AmirnaGFP.txt"> amiRNA GFP</a></td></tr>
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<tr><td>A2</td><td>amiRNA (Other allergen x)</td><td>knockdown construct for x </td><td>chloramphenicol</td><td> <a href="insert">amiRNA x</a></td></tr>
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<tr><td>A2</td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>chloramphenicol</td><td> <a href="LTP.txt">amiRNA LTP</a></td></tr>
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<tr><td>A3</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>chloramphenicol</td><td><a href="Bet1.txt"> ihpRNA Bet1</a></td></tr>
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<tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>chloramphenicol</td><td><a href="Bet1.txt"> ihpRNA Bet1</a></td></tr>
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<tr><td>A4</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td><a href="LTP.txt">ihpRNA LTP</a></td></tr>
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<tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td><a href="LTP.txt">ihpRNA LTP</a></td></tr>
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<tr><td>A5</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td><a href="Ger.txt">ihpRNA GER</a></td></tr>
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<tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td><a href="Ger.txt">ihpRNA GER</a></td></tr>
</table>
</table>
<br /><br />
<br /><br />
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<tr><td>AP3</td><td>3 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> GFP primer3</a></td></tr>
<tr><td>AP3</td><td>3 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> GFP primer3</a></td></tr>
<tr><td>AP4</td><td>4 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> GFP primer4</a></td></tr>
<tr><td>AP4</td><td>4 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> GFP primer4</a></td></tr>
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<tr><td>AP5</td><td>1 of 4 primers used to create the amiRNA Bet construct</td><td></td></tr>
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<tr><td>AP5</td><td>1 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt"> Bet1 Primer 1</a></td></tr>
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<tr><td>AP6</td><td>2 of 4 primers used to create the amiRNA Bet construct</td><td></td></tr>
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<tr><td>AP6</td><td>2 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt"> Bet1 Primer 2</a></td></tr>
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<tr><td>AP7</td><td>3 of 4 primers used to create the amiRNA Bet construct</td><td></td></tr>
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<tr><td>AP7</td><td>3 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt"> Bet1 Primer 3</a></td></tr>
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<tr><td>AP8</td><td>4 of 4 primers used to create the amiRNA Bet construct</td><td></td></tr>
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<tr><td>AP8</td><td>4 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt"> Bet1 Primer 4</a></td></tr>
<tr><td>AP9</td><td>Forward primer for Bet v1 Sense</td><td><a href="Bet1.txt"> Bet1 Sense Forward</a></td></tr>
<tr><td>AP9</td><td>Forward primer for Bet v1 Sense</td><td><a href="Bet1.txt"> Bet1 Sense Forward</a></td></tr>
<tr><td>AP10</td><td>Reverse primer for Bet v1 Sense </td><td><a href="Bet1.txt"> Bet1 Sense Reverse</a></td></tr>
<tr><td>AP10</td><td>Reverse primer for Bet v1 Sense </td><td><a href="Bet1.txt"> Bet1 Sense Reverse</a></td></tr>
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<tr><td>AP19</td><td>Forward primer for Ger Antisense</td><td><a href="Ger.txt">Ger Antisense Forward</a></td></tr>
<tr><td>AP19</td><td>Forward primer for Ger Antisense</td><td><a href="Ger.txt">Ger Antisense Forward</a></td></tr>
<tr><td>AP20</td><td>Reverse primer for Ger Antisense</td><td><a href="Ger.txt">Ger Antisense Reverse</a></td></tr>
<tr><td>AP20</td><td>Reverse primer for Ger Antisense</td><td><a href="Ger.txt">Ger Antisense Reverse</a></td></tr>
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<tr><td>AP21</td><td>Forward Primer for PDK intron</td><td></td></tr>
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<tr><td>AP21</td><td>Forward Primer for PDK intron</td><td><a href="Introns"> PDK forward</a></td></tr>
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<tr><td>AP22</td><td>Reverse Primer for PDK intron</td><td></td></tr>
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<tr><td>AP22</td><td>Reverse Primer for PDK intron</td><td><a href="Introns"> PDK reverse</a></td></tr>
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         <tr><td>AP23</td><td>Forward Primer to make amiRNA Biobrick compatible</td><td></td></tr>
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         <tr><td>AP23</td><td>Forward Primer to make amiRNA Biobrick compatible</td><td><a href="amiRNA"> Biobrick forward</a></td></tr>
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         <tr><td>AP24</td><td>Reverse Primer to make amiRNA Biobrick compatible</td><td></td></tr>
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         <tr><td>AP24</td><td>Reverse Primer to make amiRNA Biobrick compatible</td><td><a href="amiRNA"> Biobrick reverse</a></td></tr>
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         <tr><td>AP25</td><td>1 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt">LTP primer 1</a></td></tr>
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         <tr><td>AP25</td><td>1 of 4 primers used to create the amiRNA Bet construct</td><td><a href="Bet1.txt">LTP primer 1</a></td></tr>
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         <tr><td>AP26</td><td>2 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt">LTP primer 2</a></td></tr>
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         <tr><td>AP26</td><td>2 of 4 primers used to create the amiRNA Bet construct</td><td><a href="Bet1.txt">LTP primer 2</a></td></tr>
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         <tr><td>AP27</td><td>3 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt">LTP primer 3</a></td></tr>
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         <tr><td>AP27</td><td>3 of 4 primers used to create the amiRNA Bet construct</td><td><a href="Bet1.txt">LTP primer 3</a></td></tr>
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         <tr><td>AP28</td><td>4 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt">LTP primer 4</a></td></tr>
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         <tr><td>AP28</td><td>4 of 4 primers used to create the amiRNA Bet construct</td><td><a href="Bet1.txt">LTP primer 4</a></td></tr>

Latest revision as of 00:04, 27 October 2010



parts and primers

Allergy

For more information on the allergens visit our "allergen targets" page

NameBiobrick NameOriginal NameDescriptionResistanceSequence
A1
-
amiRNA GFPGFP knockdown constructchloramphenicol amiRNA GFP
A2BBa_K382017amiRNA LTP LTP knockdown constructchloramphenicol amiRNA LTP
A3BBa_K382014ihpRNA BetIntron hairpin Bet Knockdown constructchloramphenicol ihpRNA Bet1
A4BBa_K382015ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicolihpRNA LTP
A5BBa_K382019ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicolihpRNA GER


Primers

NameDescriptionSequence
AP11 of 4 primers used to create the amiRNA GFP construct GFP primer1
AP22 of 4 primers used to create the amiRNA GFP construct GFP primer2
AP33 of 4 primers used to create the amiRNA GFP construct GFP primer3
AP44 of 4 primers used to create the amiRNA GFP construct GFP primer4
AP51 of 4 primers used to create the amiRNA LTP construct Bet1 Primer 1
AP62 of 4 primers used to create the amiRNA LTP construct Bet1 Primer 2
AP73 of 4 primers used to create the amiRNA LTP construct Bet1 Primer 3
AP84 of 4 primers used to create the amiRNA LTP construct Bet1 Primer 4
AP9Forward primer for Bet v1 Sense Bet1 Sense Forward
AP10Reverse primer for Bet v1 Sense Bet1 Sense Reverse
AP11Forward primer for Bet v1 AntisenseBet1 Antisense Forward
AP12Reverse primer for Bet v1 Antisense Bet1 Antisense Reverse
AP13Forward primer for LTP Sense LTP Sense Forward
AP14Reverse primer for LTP SenseLTP Sense Reverse
AP15Forward primer for LTP AntisenseLTP Antisense Forward
AP16Reverse primer for LTP AntisenseLTP Antisense Reverse
AP17Forward primer for Ger SenseGer Sense Forward
AP18Reverse primer for Ger SenseGer Sense Reverse
AP19Forward primer for Ger AntisenseGer Antisense Forward
AP20Reverse primer for Ger AntisenseGer Antisense Reverse
AP21Forward Primer for PDK intron PDK forward
AP22Reverse Primer for PDK intron PDK reverse
AP23Forward Primer to make amiRNA Biobrick compatible Biobrick forward
AP24Reverse Primer to make amiRNA Biobrick compatible Biobrick reverse
AP251 of 4 primers used to create the amiRNA Bet constructLTP primer 1
AP262 of 4 primers used to create the amiRNA Bet constructLTP primer 2
AP273 of 4 primers used to create the amiRNA Bet constructLTP primer 3
AP284 of 4 primers used to create the amiRNA Bet constructLTP primer 4