Team:Harvard/allergy/allergens

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<p>We are using GFP as a control for amiRNA. </p>
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<h1>>GFP</h1>
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<p>GFP (green fluorescent protein) is a fluorescent protein that shows green florescence when exposed to blue light.  Because of its fluorescent property, it is commonly used as a reporter.  As a proof of principle we are knocking down GFP in a strain of Arabadopsis that is modified to express GFP.</p>
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<h2>LTP (liquid transfer protein) </h2>
<h2>LTP (liquid transfer protein) </h2>

Revision as of 05:43, 13 October 2010

meet the allergens

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>GFP

GFP (green fluorescent protein) is a fluorescent protein that shows green florescence when exposed to blue light. Because of its fluorescent property, it is commonly used as a reporter. As a proof of principle we are knocking down GFP in a strain of Arabadopsis that is modified to express GFP.

LTP (liquid transfer protein)

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Bet versions 1.1 and 1.2 (birch pollen analog)

Bet v 1 and Bet v 2 are analogs of a birch pollen protein that is, along with other plant pollen proteins, responsible for allergies in 100 million individuals worldwide. Bet v 1 is a previously identified allergen whose homologues have been found in hazel, hornbeam, and adder trees, as well as fruits such as apples, cherries, kiwis, and celery. Bet v 2 is a homologue of Bet v 1 found in Arabadopsis thaliana that we found specifically for the purpose of this summer's project.

At the molecular level, homologues of Bet v 1 share 65% to 56% homology. The structure of Bet v 1 and its homologues have an alpha helix that is surrounded by seven beta sheets that terminate in a large fork formed by two more consecutive alpha helixes. This forms a forked solvent-accessible cavity that transverses the entire molecule. See image here: http://btcpxx.che.uni-bayreuth.de/Fachgruppe_Chemie/grafik/Roesch_betv1_h.jpg

While the biological function of Bet v 1 and Bet v 2 are not entirely clear, the protein is expressed in plants during periods of stress

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Ger version 3

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