Team:Groningen/Transformation to E. coli

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(New page: '''Transformation to ''E. coli''''' *Add 10 µL of ligate to 100 µL competent cells *Put cells for 30 minutes on ice *Heat shock the cells for 45 seconds at 42 °C *Put cells on ice for ...)
 
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*Heat shock the cells for 45 seconds at 42 °C
*Heat shock the cells for 45 seconds at 42 °C
*Put cells on ice for 2 minutes
*Put cells on ice for 2 minutes
-
*Add 400 µL TY and grow for 1 hour at 37 °C
+
*Add 400 µL LB and grow for 1 hour at 37 °C
*Plate 100 µL of cells on LB agar (2%) plates
*Plate 100 µL of cells on LB agar (2%) plates

Latest revision as of 23:24, 7 October 2010

Transformation to E. coli

  • Add 10 µL of ligate to 100 µL competent cells
  • Put cells for 30 minutes on ice
  • Heat shock the cells for 45 seconds at 42 °C
  • Put cells on ice for 2 minutes
  • Add 400 µL LB and grow for 1 hour at 37 °C
  • Plate 100 µL of cells on LB agar (2%) plates