Team:Groningen/Project

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Revision as of 14:13, 26 August 2010

Schematic Overview

Surface hydrophobicity is a very useful property and is used in many applications ranging from raincoats, antifouling coatings, anti-bacterial and anti-fungal coatings and applications in the field of biomedical sciences to protection of highly sensitive sensor equipment. Hydrophobicity keeps a surface water free and thereby clean and dry, this prevents micro-organisms from fouling surfaces and corrosion from forming. Most hydrophobic coatings used today involve costly chemical coating treatments or production of expensive and sometimes toxic hydrophobic molecules. So why not create an organism that does the work for you.

The idea is to engineer a bacterium that once applied on a surface, starts forming a fast growing rigid biofilm. Completion of the biofilm will trigger the expression of hydrophobic proteins by the biofilm forming bacterium. These hydrophobic proteins will be incorporated in the rigid biofilm, causing strong hydrophobic surface activity. Exposure to UV-light could then trigger a kill switch, initiating the dying of the bacteria and discolouring of the biofilm. The result of these processes will be a surface that is coated by a rigid biofilm with embedded hydrophobic proteins, leaving the coated surface extremely hydrophobic.

Producing a hydrophobic biocoating that is self assembling, would have a lot of advantages. Firstly it is relatively cheap to apply bacteria to surfaces. And since the coating process will be done by the bacteria themselves, there is no high-tech treatment involved and there are no expensive chemicals necessary to attain the hydrophobicity. Secondly, because these hydrophobins are proteins, they are in contrast to many chemical hydrophobins non-toxic to the environment. Applications of this hydrophobic biofilm could range from non-toxic antifouling coatings on ships, antifungal coatings and corrosion and water protecting coatings.