Team:Groningen/Biobricks

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Biobricks

Here is our registry of our biobricks:

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Summary:
Below is given a short description of all the biobricks in our registry.

Chaplins

BBa_K305001 Hydrophobic protein chaplin C (chpC)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
BBa_K305002 chaplin C (chpDC) with modified sortase binding motive
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.
BBa_K305003 Hydrophobic protein chaplin E (chpE)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
BBa_K305004 Hydrophobic protein chaplin H (chpH)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
BBa_K305005 Sortase srtA
Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall

Promotors

BBa_K305006 yqxMpr
yqxM promotor effective in biofilm formation, regulates tasA production
BBa_K305007 srfApr 1
Promotor for the surfactin regulatory gene.
BBa_K305008 srfApr 2
Promotor for the surfactin regulatory gene.
BBa_K305009 srfApr1>GFP
Promotor for the surfactin regulatory gene with GFP.
BBa_K305010 srfApr2>GFP
Promotor for the surfactin regulatory gene with GFP.

Plasmid Backbone

BBa_K305011 Subtilin inducible expression plasmid for Bacillus subtilis
Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.