Team:Groningen/Biobricks

From 2010.igem.org

(Difference between revisions)
(Biobricks)
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'''Summary:'''
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'''Summary:'''<br>
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Below is given a short description of all the biobricks in our registry.<br><br>
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Below is given a short description of all the biobricks in our registry.
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'''Chaplins'''<br><br>
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<partinfo>BBa_K305001</partinfo> Hydrophobic protein chaplin C (chpC)<br>
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.<br>
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'''Chaplins'''
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<partinfo>BBa_K305002</partinfo> chaplin C (chpDC) with modified sortase binding motive<br>
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+
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis. <br>
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<partinfo>BBa_K305001</partinfo> Hydrophobic protein chaplin C (chpC)
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Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.    <br>
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<partinfo>BBa_K305003</partinfo> Hydrophobic protein chaplin E (chpE) <br>
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
+
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.<br>
-
 
+
<partinfo>BBa_K305004</partinfo> Hydrophobic protein chaplin H (chpH)<br>
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<partinfo>BBa_K305002</partinfo> chaplin C (chpDC) with modified sortase binding motive
+
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.<br>
-
 
+
<partinfo>BBa_K305005</partinfo> Sortase srtA <br>
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.  
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Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall<br><br>
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'''Promotors'''<br><br>
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Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.     
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<partinfo>BBa_K305006</partinfo> yqxMpr <br>
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yqxM promotor effective in biofilm formation, regulates tasA production<br>
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<partinfo>BBa_K305003</partinfo> Hydrophobic protein chaplin E (chpE)  
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<partinfo>BBa_K305007</partinfo> srfApr 1<br>
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Promotor for the surfactin regulatory gene.<br>
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
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<partinfo>BBa_K305008</partinfo> srfApr 2<br>
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+
Promotor for the surfactin regulatory gene.<br>
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<partinfo>BBa_K305004</partinfo> Hydrophobic protein chaplin H (chpH)
+
<partinfo>BBa_K305009</partinfo> srfApr1>GFP  <br>
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+
Promotor for the surfactin regulatory gene with GFP. <br>
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
+
<partinfo>BBa_K305010</partinfo> srfApr2>GFP<br>
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+
Promotor for the surfactin regulatory gene with GFP. <br><br>
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<partinfo>BBa_K305005</partinfo> Sortase srtA  
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'''Plasmid Backbone'''<br><br>
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+
<partinfo>BBa_K305011</partinfo> Subtilin inducible expression plasmid for Bacillus subtilis<br>
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Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall
+
-
 
+
-
 
+
-
 
+
-
'''Promotors'''
+
-
 
+
-
<partinfo>BBa_K305006</partinfo> yqxMpr  
+
-
 
+
-
yqxM promotor effective in biofilm formation, regulates tasA production
+
-
 
+
-
<partinfo>BBa_K305007</partinfo> srfApr 1
+
-
 
+
-
Promotor for the surfactin regulatory gene.
+
-
 
+
-
<partinfo>BBa_K305008</partinfo> srfApr 2
+
-
 
+
-
Promotor for the surfactin regulatory gene.
+
-
 
+
-
<partinfo>BBa_K305009</partinfo> srfApr1>GFP   
+
-
 
+
-
Promotor for the surfactin regulatory gene with GFP.
+
-
 
+
-
<partinfo>BBa_K305010</partinfo> srfApr2>GFP
+
-
 
+
-
Promotor for the surfactin regulatory gene with GFP.
+
-
 
+
-
 
+
-
 
+
-
'''Plasmid Backbone'''
+
-
 
+
-
<partinfo>BBa_K305011</partinfo> Subtilin inducible expression plasmid for Bacillus subtilis
+
-
 
+
Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was  
Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was  
constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.
constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.

Revision as of 10:44, 26 October 2010

Biobricks

Here is our registry of our biobricks:

Loading.....


Summary:
Below is given a short description of all the biobricks in our registry.

Chaplins

BBa_K305001 Hydrophobic protein chaplin C (chpC)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
BBa_K305002 chaplin C (chpDC) with modified sortase binding motive
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.
BBa_K305003 Hydrophobic protein chaplin E (chpE)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
BBa_K305004 Hydrophobic protein chaplin H (chpH)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
BBa_K305005 Sortase srtA
Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall

Promotors

BBa_K305006 yqxMpr
yqxM promotor effective in biofilm formation, regulates tasA production
BBa_K305007 srfApr 1
Promotor for the surfactin regulatory gene.
BBa_K305008 srfApr 2
Promotor for the surfactin regulatory gene.
BBa_K305009 srfApr1>GFP
Promotor for the surfactin regulatory gene with GFP.
BBa_K305010 srfApr2>GFP
Promotor for the surfactin regulatory gene with GFP.

Plasmid Backbone

BBa_K305011 Subtilin inducible expression plasmid for Bacillus subtilis
Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.