Team:Groningen/Biobricks

From 2010.igem.org

(Difference between revisions)
(Biobricks)
(Biobricks)
Line 11: Line 11:
'''Chaplins'''
'''Chaplins'''
-
BBa_K305001 Hydrophobic protein chaplin C (chpC)
+
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305001 BBa_K305001] Hydrophobic protein chaplin C (chpC)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
-
BBa_K305002 chaplin C (chpDC) with modified sortase binding motive
+
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305002 BBa_K305002] chaplin C (chpDC) with modified sortase binding motive
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.  
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.  
Line 21: Line 21:
Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.     
Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.     
-
BBa_K305003 Hydrophobic protein chaplin E (chpE)  
+
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305003 BBa_K305003] Hydrophobic protein chaplin E (chpE)  
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
-
BBa_K305004 Hydrophobic protein chaplin H (chpH)
+
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305004 BBa_K305004] Hydrophobic protein chaplin H (chpH)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
-
BBa_K305005Sortase srtA  
+
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305005 BBa_K305005] Sortase srtA  
Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall
Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall
Line 37: Line 37:
'''Promotors'''
'''Promotors'''
-
BBa_K305006 yqxMpr  
+
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305006 BBa_K305006] yqxMpr  
yqxM promotor effective in biofilm formation, regulates tasA production
yqxM promotor effective in biofilm formation, regulates tasA production
-
BBa_K305007 srfApr 1
+
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305007 BBa_K305007] srfApr 1
Promotor for the surfactin regulatory gene.
Promotor for the surfactin regulatory gene.
-
 
+
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305008 BBa_K305008] srfApr 2
-
BBa_K305008 srfApr 2
+
Promotor for the surfactin regulatory gene.
Promotor for the surfactin regulatory gene.
-
BBa_K305009 srfApr1>GFP   
+
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305009 BBa_K305009] srfApr1>GFP   
Promotor for the surfactin regulatory gene with GFP.
Promotor for the surfactin regulatory gene with GFP.
-
BBa_K305010 srfApr2>GFP
+
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305010 BBa_K305010] srfApr2>GFP
Promotor for the surfactin regulatory gene with GFP.
Promotor for the surfactin regulatory gene with GFP.
Line 61: Line 60:
'''Plasmid Backbone'''
'''Plasmid Backbone'''
-
BBa_K305011 Subtilin inducible expression plasmid for Bacillus subtilis
+
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305011 BBa_K305011] Subtilin inducible expression plasmid for Bacillus subtilis
Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was  
Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was  
constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.
constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.

Revision as of 10:05, 25 October 2010

Biobricks

Here is our registry of our biobricks:

Loading.....

Summary:

Below is given a short description of all the biobricks in our registry.


Chaplins

BBa_K305001 Hydrophobic protein chaplin C (chpC)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

BBa_K305002 chaplin C (chpDC) with modified sortase binding motive

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.

BBa_K305003 Hydrophobic protein chaplin E (chpE)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

BBa_K305004 Hydrophobic protein chaplin H (chpH)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

BBa_K305005 Sortase srtA

Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall


Promotors

BBa_K305006 yqxMpr

yqxM promotor effective in biofilm formation, regulates tasA production

BBa_K305007 srfApr 1

Promotor for the surfactin regulatory gene. BBa_K305008 srfApr 2

Promotor for the surfactin regulatory gene.

BBa_K305009 srfApr1>GFP

Promotor for the surfactin regulatory gene with GFP.

BBa_K305010 srfApr2>GFP

Promotor for the surfactin regulatory gene with GFP.


Plasmid Backbone

BBa_K305011 Subtilin inducible expression plasmid for Bacillus subtilis

Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.