"
Team:Groningen/Biobricks
From 2010.igem.org
(→Biobricks) |
(→Biobricks) |
||
| Line 11: | Line 11: | ||
'''Chaplins''' | '''Chaplins''' | ||
| - | BBa_K305001 Hydrophobic protein chaplin C (chpC) | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K305001 BBa_K305001] Hydrophobic protein chaplin C (chpC) |
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis. | Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis. | ||
| - | BBa_K305002 chaplin C (chpDC) with modified sortase binding motive | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K305002 BBa_K305002] chaplin C (chpDC) with modified sortase binding motive |
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis. | Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis. | ||
| Line 21: | Line 21: | ||
Modified C-terminal Sortase(SrtA from S. aureus.) recognition site. | Modified C-terminal Sortase(SrtA from S. aureus.) recognition site. | ||
| - | BBa_K305003 Hydrophobic protein chaplin E (chpE) | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K305003 BBa_K305003] Hydrophobic protein chaplin E (chpE) |
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis. | Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis. | ||
| - | BBa_K305004 Hydrophobic protein chaplin H (chpH) | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K305004 BBa_K305004] Hydrophobic protein chaplin H (chpH) |
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis. | Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis. | ||
| - | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K305005 BBa_K305005] Sortase srtA | |
Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall | Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall | ||
| Line 37: | Line 37: | ||
'''Promotors''' | '''Promotors''' | ||
| - | BBa_K305006 yqxMpr | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K305006 BBa_K305006] yqxMpr |
yqxM promotor effective in biofilm formation, regulates tasA production | yqxM promotor effective in biofilm formation, regulates tasA production | ||
| - | BBa_K305007 srfApr 1 | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K305007 BBa_K305007] srfApr 1 |
Promotor for the surfactin regulatory gene. | Promotor for the surfactin regulatory gene. | ||
| - | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K305008 BBa_K305008] srfApr 2 | |
| - | BBa_K305008 srfApr 2 | + | |
Promotor for the surfactin regulatory gene. | Promotor for the surfactin regulatory gene. | ||
| - | BBa_K305009 srfApr1>GFP | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K305009 BBa_K305009] srfApr1>GFP |
Promotor for the surfactin regulatory gene with GFP. | Promotor for the surfactin regulatory gene with GFP. | ||
| - | BBa_K305010 srfApr2>GFP | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K305010 BBa_K305010] srfApr2>GFP |
Promotor for the surfactin regulatory gene with GFP. | Promotor for the surfactin regulatory gene with GFP. | ||
| Line 61: | Line 60: | ||
'''Plasmid Backbone''' | '''Plasmid Backbone''' | ||
| - | BBa_K305011 Subtilin inducible expression plasmid for Bacillus subtilis | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K305011 BBa_K305011] Subtilin inducible expression plasmid for Bacillus subtilis |
Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was | Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was | ||
constructed to express proteins present in RCF 10 BioBricks upon subtilin induction. | constructed to express proteins present in RCF 10 BioBricks upon subtilin induction. | ||
Revision as of 10:05, 25 October 2010
Biobricks
Here is our registry of our biobricks: <groupparts>iGEM010 Groningen</groupparts>
Summary:
Below is given a short description of all the biobricks in our registry.
Chaplins
BBa_K305001 Hydrophobic protein chaplin C (chpC)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
BBa_K305002 chaplin C (chpDC) with modified sortase binding motive
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.
BBa_K305003 Hydrophobic protein chaplin E (chpE)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
BBa_K305004 Hydrophobic protein chaplin H (chpH)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
BBa_K305005 Sortase srtA
Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall
Promotors
BBa_K305006 yqxMpr
yqxM promotor effective in biofilm formation, regulates tasA production
BBa_K305007 srfApr 1
Promotor for the surfactin regulatory gene. BBa_K305008 srfApr 2
Promotor for the surfactin regulatory gene.
BBa_K305009 srfApr1>GFP
Promotor for the surfactin regulatory gene with GFP.
BBa_K305010 srfApr2>GFP
Promotor for the surfactin regulatory gene with GFP.
Plasmid Backbone
BBa_K305011 Subtilin inducible expression plasmid for Bacillus subtilis
Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.
