Team:Groningen/Biobricks

From 2010.igem.org

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== Biobricks ==  
== Biobricks ==  
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Here is our registry of our biobricks:
<groupparts>iGEM010 Groningen</groupparts>
<groupparts>iGEM010 Groningen</groupparts>
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'''Summary:'''
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Below is given a short description of all the biobricks in our registry.
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'''Chaplins'''
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BBa_K305001 Hydrophobic protein chaplin C (chpC)
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
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BBa_K305002 chaplin C (chpDC) with modified sortase binding motive
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
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Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.   
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BBa_K305003 Hydrophobic protein chaplin E (chpE)
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
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BBa_K305004 Hydrophobic protein chaplin H (chpH)
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Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
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BBa_K305005Sortase srtA
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Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall
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'''Promotors'''
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BBa_K305006 yqxMpr
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yqxM promotor effective in biofilm formation, regulates tasA production
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BBa_K305007 srfApr 1
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Promotor for the surfactin regulatory gene.
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BBa_K305008 srfApr 2
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Promotor for the surfactin regulatory gene.
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BBa_K305009 srfApr1>GFP 
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Promotor for the surfactin regulatory gene with GFP.
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BBa_K305010 srfApr2>GFP
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Promotor for the surfactin regulatory gene with GFP.
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'''Plasmid Backbone'''
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BBa_K305011 Subtilin inducible expression plasmid for Bacillus subtilis
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Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was
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constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.

Revision as of 20:56, 24 October 2010

Biobricks

Here is our registry of our biobricks:

Loading.....

Summary:

Below is given a short description of all the biobricks in our registry.


Chaplins

BBa_K305001 Hydrophobic protein chaplin C (chpC)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

BBa_K305002 chaplin C (chpDC) with modified sortase binding motive

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.

BBa_K305003 Hydrophobic protein chaplin E (chpE)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

BBa_K305004 Hydrophobic protein chaplin H (chpH)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

BBa_K305005Sortase srtA

Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall


Promotors

BBa_K305006 yqxMpr

yqxM promotor effective in biofilm formation, regulates tasA production

BBa_K305007 srfApr 1

Promotor for the surfactin regulatory gene.

BBa_K305008 srfApr 2

Promotor for the surfactin regulatory gene.

BBa_K305009 srfApr1>GFP

Promotor for the surfactin regulatory gene with GFP.

BBa_K305010 srfApr2>GFP

Promotor for the surfactin regulatory gene with GFP.


Plasmid Backbone

BBa_K305011 Subtilin inducible expression plasmid for Bacillus subtilis

Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.