Team:Groningen/Antibiotics Concentrationas and Organisms

From 2010.igem.org

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'''Concentrations for antibiotics '''
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'''Antibiotics'''
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'''Antibiotic'''''' Stock''' '''  E.coli '''    '''  B.subtilis'''
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[[Ampicillin]]
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*[[Ampicillin]] (Amp) (in H2O)     50 mg/ml (4 °C)     100(μg/ml) (:500)
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100 mg/ml Ampicillin (1000x) Stock
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*1 g of Ampicillin sodium salt in 10 mL of demiwater (or 50% EtOH)
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*Add NaOH or KOH to allow the Ampicillin to dissolve
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*Filter sterilize 0.2 μm filter and aliquot
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*Store -20 °C
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*[[Kanamycin]] (Km)             10 mg/ml (4 °C)     20 (μg/ml) (:500)                    10 (μg/ml) (:100)
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[[Chloramphenicol]]
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*[[Chloramphenicol]](Cm) (50% EtOH)    5 mg/ml (-18 °C)     10-15 (μg/ml) (:500/333)         5(μg/ml) (:1000)
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35 mg/ml Chloramphenicol (1000x) Stock
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*0.35 g in 10 mL 100% EtOH
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*Filter sterilize 0.2 μm filter and aliquot
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*Store -20 °C
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[[Kanamycin]]
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50 mg/ml Kanamycin (1000x) Stock
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*500 mg in 10 mL demi water
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*Filter sterilize 0.2 μm filter and aliquot
 +
*Store -20 °C

Latest revision as of 15:42, 25 October 2010

Antibiotics

Ampicillin

100 mg/ml Ampicillin (1000x) Stock

  • 1 g of Ampicillin sodium salt in 10 mL of demiwater (or 50% EtOH)
  • Add NaOH or KOH to allow the Ampicillin to dissolve
  • Filter sterilize 0.2 μm filter and aliquot
  • Store -20 °C

Chloramphenicol

35 mg/ml Chloramphenicol (1000x) Stock

  • 0.35 g in 10 mL 100% EtOH
  • Filter sterilize 0.2 μm filter and aliquot
  • Store -20 °C

Kanamycin

50 mg/ml Kanamycin (1000x) Stock

  • 500 mg in 10 mL demi water
  • Filter sterilize 0.2 μm filter and aliquot
  • Store -20 °C