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Revision as of 17:19, 9 September 2010 (view source) Neima (Talk | contribs) (New page: '''Concentrations for antibiotics ''' '''Antibiotic''' ''' Stock''' ''' E.coli ''' ''' B.subtilis''' *Ampicillin (Amp) (in H2O) 50 mg/ml (4 °C...) ← Older edit		Latest revision as of 15:42, 25 October 2010 (view source) Neima (Talk | contribs) m
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-	'''Concentrations for antibiotics '''	+	'''Antibiotics'''
-	'''Antibiotic'''               ''' Stock'''     '''  E.coli '''              '''  B.subtilis'''	+	[[Ampicillin]]
-	*Ampicillin (Amp) (in H2O)     50 mg/ml (4 °C)     100(μg/ml) (:500)	+	100 mg/ml Ampicillin (1000x) Stock
		+	*1 g of Ampicillin sodium salt in 10 mL of demiwater (or 50% EtOH)
		+	*Add NaOH or KOH to allow the Ampicillin to dissolve
		+	*Filter sterilize 0.2 μm filter and aliquot
		+	*Store -20 °C
-	*Kanamycin (Km)             10 mg/ml (4 °C)     20 (μg/ml) (:500)                    10 (μg/ml) (:100)	+	[[Chloramphenicol]]
-	*Spectinomycin (Spec) (in H2O)     10 mg/ml (4 °C)     100(μg/ml) (:100)                 100 (μg/ml) (:100)	+	35 mg/ml Chloramphenicol (1000x) Stock
		+	*0.35 g in 10 mL 100% EtOH
		+	*Filter sterilize 0.2 μm filter and aliquot
		+	*Store -20 °C
-	*Erythromycin (Ery) E.coli stock    10 mg/ml (-18 °C)     150 (μg/ml) (:66.6)	+	[[Kanamycin]]
-	*Erythromycin(Ery) B.subtilis stock  1 mg/ml (-18 °C)                                 2-5 (μg/ml)	+	50 mg/ml Kanamycin (1000x) Stock
-		+	*500 mg in 10 mL demi water
-	*Tetracyclin (Tet) (50% EtOH)     3 mg/ml (-18 °C)     12 (μg/ml) (:250)                 6(μg/ml) (:500)	+	*Filter sterilize 0.2 μm filter and aliquot
-		+	*Store -20 °C
-	*Chloramphenicol (Cm) (50% EtOH)    5 mg/ml (-18 °C)     10-15 (μg/ml) (:500/333)         5(μg/ml) (:1000)	+
-		+
-	*Lyncomycin                     12.5 mg/ml (4 °C)                                 12.5 (μg/ml) (1:1000)	+
-		+
-	*Hygromycin                     50 mg/ml (4 °C)                                 125 (μg/ml) (:400)	+

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Latest revision as of 15:42, 25 October 2010

Antibiotics

Ampicillin

100 mg/ml Ampicillin (1000x) Stock

• 1 g of Ampicillin sodium salt in 10 mL of demiwater (or 50% EtOH)
• Add NaOH or KOH to allow the Ampicillin to dissolve
• Filter sterilize 0.2 μm filter and aliquot
• Store -20 °C

Chloramphenicol

35 mg/ml Chloramphenicol (1000x) Stock

• 0.35 g in 10 mL 100% EtOH
• Filter sterilize 0.2 μm filter and aliquot
• Store -20 °C

Kanamycin

50 mg/ml Kanamycin (1000x) Stock

• 500 mg in 10 mL demi water
• Filter sterilize 0.2 μm filter and aliquot
• Store -20 °C