Team:Groningen/6 September 2010

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As a follow up experiment for the last [http://2010.igem.org/Team:Groningen/23_August_2010 biofilm induction ] ''Bacillus subtilis'' wit Chaplin E and chaplin H were grown while continuously adding subtilin to keep expression up. After 3hrs of growth they where induced with 1%subtilin. Every hour an additional 0.5%subtilin was added. As a negative control uninduced strains where used. Furthermore shaking cultures (induced and uninduced) where grown simultaneously to check OD levels. Only the chaplin E strain showed reduced growth, but no differences other than retarded growth were visible.
As a follow up experiment for the last [http://2010.igem.org/Team:Groningen/23_August_2010 biofilm induction ] ''Bacillus subtilis'' wit Chaplin E and chaplin H were grown while continuously adding subtilin to keep expression up. After 3hrs of growth they where induced with 1%subtilin. Every hour an additional 0.5%subtilin was added. As a negative control uninduced strains where used. Furthermore shaking cultures (induced and uninduced) where grown simultaneously to check OD levels. Only the chaplin E strain showed reduced growth, but no differences other than retarded growth were visible.
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'''Modellers:''' Work on genexpression model ''Joël, Laura, Djoke''

Revision as of 12:32, 24 October 2010

Assembly of biobricks in pSB1C3-backbone for submission to parts registry by Maarten

Control restrictions with EcoRI and PstI on constructs:

2 µL construct
2 µL buffer O
0,5 µL EcoRI
0,5 µL PstI
15 µL MQ
Construct Expected band sizes (bp)
pSB1C3-C 2031, 848
pSB1C3-DC 2031, 872
pSB1C3-E 2031, 317
pSB1C3-H 2031, 302
pSB1C3-Sortase 2031, 689
pSB1C3-RFP 2031, 1069

The gel that was run did not show any DNA present on the gel. Experiment was continued on 8 September.

Expression experiments -David & Peter

Two expression experiments were done this week.


Continous induction of chaplins

As a follow up experiment for the last biofilm induction Bacillus subtilis wit Chaplin E and chaplin H were grown while continuously adding subtilin to keep expression up. After 3hrs of growth they where induced with 1%subtilin. Every hour an additional 0.5%subtilin was added. As a negative control uninduced strains where used. Furthermore shaking cultures (induced and uninduced) where grown simultaneously to check OD levels. Only the chaplin E strain showed reduced growth, but no differences other than retarded growth were visible.

Modellers: Work on genexpression model Joël, Laura, Djoke