Team:Groningen/30 August 2010


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iGEM Groningen 2010

pushing coatings into a greener future

Week 35

SDS-page - Chaplin ladder - Peter

Various biobricks where transformed in order to have them ready for eventual further experiments

The following Biobricks where transformed into the pNZ8901 backbone

Fluorescent proteins: GFP, Cyan, Yellow

Promoters: pBad/araC promoter, lacI regulated promoter

Modellers: Write pieces about modelling for the wiki, do some more literature research and modelling. Joël, Laura, Djoke Start writing information standard Arend Find information about the killswitch system and write an introduction for the wiki about killswitches. Laura


Left Groningen for internship, so no more labwork, but mentally supported the rest of the team that contuinued.


Colonies visible with possible pNZ-CCH1 or pNZ-dCS. 8 Colonies were grown overnight in LB medium. The cultures were miniprepped and checked on gel. On gel neither of the products was visible and nanodrop revealed low concentrations in the miniprep.



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