Team:Groningen/27 September 2010


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iGEM Groningen 2010

pushing coatings into a greener future

Week 29

Chaplin ladder on gel-Peter

The Yqxm and the Srfa (both variants)promoters where extracted from genomic Bacillus Subtilis DNA via PCR and inserted into the PNZ8901 plasmid. All transformations where done in Lactococcus Lactis. Restriction analysis was performed to check the constructs. The concentrations where determined with the Nanodrop

Srfa 1 Srfa 2 Yqxm
Clone 1 75.3mg/ml 78.9mg/ml 72.0mg/mL
Clone 2 25.6mg/ml 40.2mg/ml 57.6mg/mL
Clone 3 44.4mg/ml False positive False positive



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