Team:Groningen/27 September 2010

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(New page: {{Team:Groningen/Header}} '''Week 29''' '''Chaplin ladder on gel'''-''Peter'' {{Team:Groningen/Footer}})
 
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'''Chaplin ladder on gel'''-''Peter''
'''Chaplin ladder on gel'''-''Peter''
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The Yqxm and the Srfa (both variants)promoters where extracted from genomic ''Bacillus Subtilis'' DNA via PCR and inserted into the PNZ8901 plasmid. All transformations where done in ''Lactococcus Lactis''.
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Restriction analysis was performed to check the constructs. The concentrations where determined with the Nanodrop
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 +
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{| border="1"
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|
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| Srfa 1
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| Srfa 2
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| Yqxm
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|-
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| Clone 1
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| 75.3mg/ml
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| 78.9mg/ml
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| 72.0mg/mL
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|-
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| Clone 2
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| 25.6mg/ml
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| 40.2mg/ml
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| 57.6mg/mL
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|-
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| Clone 3
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| 44.4mg/ml
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| False positive
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| False positive
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|}
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 +
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'''Laura'''
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Debugging the matlab model of killswitch and gene expression.
{{Team:Groningen/Footer}}
{{Team:Groningen/Footer}}
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'''David'''
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<br>
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Biofilm paste experiment
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<br>
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Growing biofilms on sterilized ceramics that have been coated with athin layer of TY medium with corn starch.
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<br>
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Mix: 30 mg cornstarch/ml TY 1% agar medium and 0.5% glucose
 +
 +
After dissolving the agar and addition of corn starch teh pieces of cermics we added an taken out 
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[[Image:ceramic coating gr1.jpg]]
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[[Image:ceramic coating gr1.jpg]]
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[[Image:ceramic coating gr1.jpg]]
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[[Image:ceramic coating gr1.jpg]]
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 +
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Expression experiment:
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[[Image:growthchaplineEN.jpg]]
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ThT measurement of EH induced together with C non induced(this due to a mixup during he experiment), EH non induced, C induced
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 +
[[Image:disrupted last measurement gr.jpg]]

Latest revision as of 01:59, 28 October 2010

iGEM Groningen 2010

Hydrophobofilm
pushing coatings into a greener future

Week 29

Chaplin ladder on gel-Peter


The Yqxm and the Srfa (both variants)promoters where extracted from genomic Bacillus Subtilis DNA via PCR and inserted into the PNZ8901 plasmid. All transformations where done in Lactococcus Lactis. Restriction analysis was performed to check the constructs. The concentrations where determined with the Nanodrop


Srfa 1 Srfa 2 Yqxm
Clone 1 75.3mg/ml 78.9mg/ml 72.0mg/mL
Clone 2 25.6mg/ml 40.2mg/ml 57.6mg/mL
Clone 3 44.4mg/ml False positive False positive


Laura

Debugging the matlab model of killswitch and gene expression.

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David
Biofilm paste experiment
Growing biofilms on sterilized ceramics that have been coated with athin layer of TY medium with corn starch.
Mix: 30 mg cornstarch/ml TY 1% agar medium and 0.5% glucose

After dissolving the agar and addition of corn starch teh pieces of cermics we added an taken out

Ceramic coating gr1.jpg Ceramic coating gr1.jpg Ceramic coating gr1.jpg Ceramic coating gr1.jpg


Expression experiment:

File:GrowthchaplineEN.jpg

ThT measurement of EH induced together with C non induced(this due to a mixup during he experiment), EH non induced, C induced

Disrupted last measurement gr.jpg