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Team:Groningen/27 September 2010
From 2010.igem.org
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'''Chaplin ladder on gel'''-''Peter'' | '''Chaplin ladder on gel'''-''Peter'' | ||
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The Yqxm and the Srfa (both variants)promoters where extracted from genomic ''Bacillus Subtilis'' DNA via PCR and inserted into the PNZ8901 plasmid. All transformations where done in ''Lactococcus Lactis''. | The Yqxm and the Srfa (both variants)promoters where extracted from genomic ''Bacillus Subtilis'' DNA via PCR and inserted into the PNZ8901 plasmid. All transformations where done in ''Lactococcus Lactis''. | ||
| + | Restriction analysis was performed to check the constructs. The concentrations where determined with the Nanodrop | ||
| + | |||
| + | |||
| + | {| border="1" | ||
| + | | | ||
| + | | Srfa 1 | ||
| + | | Srfa 2 | ||
| + | | Yqxm | ||
| + | |- | ||
| + | | Clone 1 | ||
| + | | 75.3mg/ml | ||
| + | | 78.9mg/ml | ||
| + | | 72.0mg/mL | ||
| + | |- | ||
| + | | Clone 2 | ||
| + | | 25.6mg/ml | ||
| + | | 40.2mg/ml | ||
| + | | 57.6mg/mL | ||
| + | |- | ||
| + | | Clone 3 | ||
| + | | 44.4mg/ml | ||
| + | | False positive | ||
| + | | False positive | ||
| + | |} | ||
| + | |||
| + | |||
| + | '''Laura''' | ||
| + | |||
| + | Debugging the matlab model of killswitch and gene expression. | ||
{{Team:Groningen/Footer}} | {{Team:Groningen/Footer}} | ||
| + | |||
| + | '''David''' | ||
| + | <br> | ||
| + | Biofilm paste experiment | ||
| + | <br> | ||
| + | Growing biofilms on sterilized ceramics that have been coated with athin layer of TY medium with corn starch. | ||
| + | <br> | ||
| + | Mix: 30 mg cornstarch/ml TY 1% agar medium and 0.5% glucose | ||
| + | |||
| + | After dissolving the agar and addition of corn starch teh pieces of cermics we added an taken out | ||
| + | |||
| + | [[Image:ceramic coating gr1.jpg]] | ||
| + | [[Image:ceramic coating gr1.jpg]] | ||
| + | [[Image:ceramic coating gr1.jpg]] | ||
| + | [[Image:ceramic coating gr1.jpg]] | ||
| + | |||
| + | |||
| + | Expression experiment: | ||
| + | |||
| + | [[Image:growthchaplineEN.jpg]] | ||
| + | |||
| + | ThT measurement of EH induced together with C non induced(this due to a mixup during he experiment), EH non induced, C induced | ||
| + | |||
| + | [[Image:disrupted last measurement gr.jpg]] | ||
Latest revision as of 01:59, 28 October 2010
Hydrophobofilm
pushing coatings into a greener future
Week 29
Chaplin ladder on gel-Peter
The Yqxm and the Srfa (both variants)promoters where extracted from genomic Bacillus Subtilis DNA via PCR and inserted into the PNZ8901 plasmid. All transformations where done in Lactococcus Lactis.
Restriction analysis was performed to check the constructs. The concentrations where determined with the Nanodrop
| Srfa 1 | Srfa 2 | Yqxm | |
| Clone 1 | 75.3mg/ml | 78.9mg/ml | 72.0mg/mL |
| Clone 2 | 25.6mg/ml | 40.2mg/ml | 57.6mg/mL |
| Clone 3 | 44.4mg/ml | False positive | False positive |
Laura
Debugging the matlab model of killswitch and gene expression.
David
Biofilm paste experiment
Growing biofilms on sterilized ceramics that have been coated with athin layer of TY medium with corn starch.
Mix: 30 mg cornstarch/ml TY 1% agar medium and 0.5% glucose
After dissolving the agar and addition of corn starch teh pieces of cermics we added an taken out
Expression experiment:
ThT measurement of EH induced together with C non induced(this due to a mixup during he experiment), EH non induced, C induced
