Team:Groningen/23 August 2010


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iGEM Groningen 2010

pushing coatings into a greener future

Expression experiment - David & Peter
The second expression experiment.
Analasys was done by THT and SDS page.
Biofilm formation experiment

Bacillus Subtilis cultures were grown overnight in a Petridish containing 20mL of LB medium and a piece of ceramics to spurr the biofilm formation. The following cultures wehre grown:

Bacillus Subtilis, Bacillus Subtilis ΔTasA, Bacillus Subtilis wit chaplin E1, Bacillus Subtilis with chaplin H1, Bacillus Subtilis ΔTasA with chaplin E1, Bacillus Subtilis ΔTasA with chaplin H1

All cultures where inoculated with 100uL of overnight culture. All cultures where run in duplicate, one induced with 1%subtilin, and one without subtilin as a negative control.



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