Team:Groningen/11 October 2010

From 2010.igem.org

(Difference between revisions)
(New page: '''Laura''' Debugging the matlab model for gene expression and killswitch. Find more information about constants used in the gene expression model.)
 
(One intermediate revision not shown)
Line 2: Line 2:
Debugging the matlab model for gene expression and killswitch. Find more information about constants used in the gene expression model.
Debugging the matlab model for gene expression and killswitch. Find more information about constants used in the gene expression model.
 +
 +
'''Peter & David'''
 +
<br>
 +
Expression experiment on new transformants: E+H1, E+H2, C1, C2, E and H mix 4 clones.
 +
Growing the strains from overnight cultures in 5 ml of TY medium at 37 degrees.
 +
 +
'''David'''
 +
<br>
 +
Biofilm paste experiment
 +
<br>
 +
Growing biofilms on sterilized ceramics that have been coated with athin layer of TY medium with corn starch.
 +
<br>
 +
Mix: 30 mg cornstarch/ml TY 1% agar medium and 0.5% glucose
 +
 +
After dissolving the agar and addition of corn starch teh pieces of cermics we added an taken out 
 +
 +
[[Image:ceramic coating gr1.jpg]]
 +
[[Image:ceramic coating gr1.jpg]]
 +
[[Image:ceramic coating gr1.jpg]]
 +
[[Image:ceramic coating gr1.jpg]]
 +
 +
 +
Expression experiment:
 +
 +
[[Image:growthchaplineEN.jpg]]
 +
 +
ThT measurement of EH induced together with C non induced(this due to a mixup during he experiment), EH non induced, C induced
 +
 +
[[Image:disrupted last measurement gr.jpg]]

Latest revision as of 02:02, 28 October 2010

Laura

Debugging the matlab model for gene expression and killswitch. Find more information about constants used in the gene expression model.

Peter & David
Expression experiment on new transformants: E+H1, E+H2, C1, C2, E and H mix 4 clones. Growing the strains from overnight cultures in 5 ml of TY medium at 37 degrees.

David
Biofilm paste experiment
Growing biofilms on sterilized ceramics that have been coated with athin layer of TY medium with corn starch.
Mix: 30 mg cornstarch/ml TY 1% agar medium and 0.5% glucose

After dissolving the agar and addition of corn starch teh pieces of cermics we added an taken out

Ceramic coating gr1.jpg Ceramic coating gr1.jpg Ceramic coating gr1.jpg Ceramic coating gr1.jpg


Expression experiment:

File:GrowthchaplineEN.jpg

ThT measurement of EH induced together with C non induced(this due to a mixup during he experiment), EH non induced, C induced

Disrupted last measurement gr.jpg