Team:Gothenburg-Sweden/test

From 2010.igem.org

(Difference between revisions)
Line 28: Line 28:
     padding-top: 10px;
     padding-top: 10px;
     padding-right:-200px;
     padding-right:-200px;
-
      
+
     margin-left: 20px;
     text-align:center;
     text-align:center;
     background: url(http://2010.igem.org/wiki/images/6/64/Title_center.png) left top;
     background: url(http://2010.igem.org/wiki/images/6/64/Title_center.png) left top;
Line 121: Line 121:
     margin: 0;
     margin: 0;
}
}
-
 
-
 
-
p {
 
-
font-size:12px;
 
-
 
-
}
 
-
 
Line 134: Line 127:
   margin:0;
   margin:0;
   padding-left:10%;
   padding-left:10%;
-
   background:  url(../bg1.png) top center repeat-y;
+
   background:  url(http://2010.igem.org/wiki/images/3/3a/Bg.png) top center repeat-y;
}  
}  
Line 186: Line 179:
background: url(http://2010.igem.org/wiki/images/1/10/CatCon.png) top repeat-y;
background: url(http://2010.igem.org/wiki/images/1/10/CatCon.png) top repeat-y;
     min-height:100px;
     min-height:100px;
-
     border: 1px solid #0485B0;
+
     border: 1px solid #0485B0;
}
}
 +
#news {
 +
  padding-top:0px;
 +
  background: url(http://2010.igem.org/wiki/images/1/10/CatCon.png) left top repeat-y;
 +
  min-height:100px;
 +
  border: 1px dotted #0485B0; 
 +
 +
}
 +
#news p{
 +
    color:#07374E;
 +
    font-weight:normal;
 +
    font-size: 11px;
 +
text-align:left;
 +
    text-decoration:none;
 +
   
 +
    padding: 10px 5px 0px 15px;
 +
 +
   
 +
display:block;
 +
}
 +
 +
#news a:hover {
 +
border-bottom: 1px dotted #06639e;
 +
}
.categories a{
.categories a{
Line 196: Line 212:
     text-decoration: none;
     text-decoration: none;
     text-align:left;
     text-align:left;
-
     padding: 3px 0 3px 20px;
+
     padding: 6px 0px 6px 20px;
     font-size:12px;
     font-size:12px;
     padding-top:5px;
     padding-top:5px;
Line 205: Line 221:
.categories a:hover{
.categories a:hover{
   text-decoration:none;
   text-decoration:none;
 +
   font-size:12px;
   font-size:12px;
   font-weight:700;
   font-weight:700;
Line 267: Line 284:
text-align: center;
text-align: center;
text-decoration: none;
text-decoration: none;
-
font:  14px Rockwell ;
+
font-size15px;
-
font-weight:500;
+
font-family: Rockwell;
 +
font-weight:normal;
}
}
Line 275: Line 293:
}
}
#active a{
#active a{
-
background: url(http://2010.igem.org/wiki/images/0/0c/MActive2.png) top center no-repeat;
+
background: url(http://2010.igem.org/wiki/images/0/0c/MActive2.png) top center repeat-y;
-
  color:#FFFFFF;
+
    color:#FFFFFF;
-
font: 13px arial;
+
font: 13px arial !important;
-
font-weight:600;
+
font-weight:600 !important;
}
}
#sddm li a:hover
#sddm li a:hover
-
{ background: url(http://2010.igem.org/wiki/images/5/5e/MaHover.png) top center no-repeat;
+
{ background: url(http://2010.igem.org/wiki/images/0/0c/MActive2.png) top center repeat-y;
color:#fff;
color:#fff;
font:  13px arial;
font:  13px arial;
Line 332: Line 350:
#text{
#text{
     text-align:left;
     text-align:left;
-
+
width:100%;
-
margin:10px 10px 10px 20px;
+
margin: 0px 10px 10px 20px;
     background: #fff ;
     background: #fff ;
 +
}
 +
 +
#text p
 +
{
 +
  padding: 10px 10px 10px 25px;
 +
  font-family: "Times New Roman";
 +
  font-size:13px;
 +
  line-height:19px;
}
}
.logo{
.logo{
float:left;
float:left;
-
padding: 10px 10px 10px 10px;
+
padding: 10px 20px 10px 20px;
  }
  }
Line 350: Line 376:
}
}
-
#center .read{
 
-
    height: 26px;
 
-
    padding-top: 3px;
 
-
    background: url(images/read.png);
 
-
    background-repeat: no-repeat;
 
-
    background-position: top;
 
-
}
 
-
#center .read a{
+
 
-
    color: #ffffff;
+
 
-
    font-weight:normal;
+
#team {
-
    font-style: italic;
+
padding: 20px 10px 20px 30px;
-
    font-size: 12px;
+
-
  /* padding-right: 15px;*/
+
-
    text-decoration:none;
+
}
}
Line 498: Line 514:
</style>
</style>
-
<style type="text/css">
+
<style type="text/css"></style>
-
 
+
-
 
+
-
</style>
+
<script type="text/javascript">
<script type="text/javascript">
// Copyright 2006-2007 javascript-array.com
// Copyright 2006-2007 javascript-array.com
Line 644: Line 657:
<div style="clear:both"></div></div>
<div style="clear:both"></div></div>
<div id="text">
<div id="text">
-
<p>We are a team of 8 students from Chalmers University of Technology who  will represent Gothenburg, SWEDEN in this year’s iGEM competition. We  have started with a promising idea that combines the cutting edge  technologies available in the field of Synthetic Biology. Our research  basically aims to constructing a reporter mechanism for cellular stress  in yeast by tagging protein and peptides affiliated with the stress  activated SNF1 complex with fluorescent markers. </p>  
+
</br>  
-
                       
+
-
      </div>       
+
-
         
+
-
+
-
+
-
                            <div id="myTitle">Chalmers University of Technology  </div>
+
                         <div class="logo"> <img src="http://2010.igem.org/wiki/images/a/a5/Chalmers_c_logo.jpg" width="133" height="152" align="left"></div>
                         <div class="logo"> <img src="http://2010.igem.org/wiki/images/a/a5/Chalmers_c_logo.jpg" width="133" height="152" align="left"></div>
-
                    <div id="text"> 
+
                   
-
+
                               <p>
                               <p>
-
                                 <strong>Chalmers in central Göteborg on the Swedish West Coast</strong></p>  
+
                                 <strong>CHALMERS UNIVERSITY OF TECHNOLOGY, GOTHENBURG</strong></p>  
                         
                         
-
                               <p>Chalmers is a university of technology in which research and  teaching  are conducted on a broad front within technology, natural  science and  architecture. Our inspiration lies in the joy of discovery  and the  desire to learn. Underlying everything we do is a wish to   contribute to  sustainable development both in Sweden and world-wide.<br>  
+
                               <p>We are a team of 8 students from Chalmers University of Technology    who will represent Gothenburg, SWEDEN in this year’s iGEM competition.    We have started with a promising idea that combines cutting edge    technologies available in the field of Synthetic Biology. Our research    basically aims to constructing an optical reporter mechanism for    cellular stress by tagging the stress activated SNF1 complex in yeast    with fluorescent markers.<br>  
                                   <br>  
                                   <br>  
-
                                Our  research work deals ultimately with improving people’s   conditions, and   we often cross traditional boundaries in order to   solve the problems of   the future. Chalmers has become strong within   several areas of science,   and some of our research leads its field   internationally. We wish in   particular to develop and strengthen our   research in the fields of   bioscience, information technology,   environmental science and  nanotechnology.<br>  
+
                              The project is performed with two main experimental   pathways: Both   experimental setups will utilize FRET to visualize the  conformational  change that is the result SNF1 activation. The first  approach consists   of fusing fluorescent two proteins, EYFP and ECFP,  with the SNF1   complex. The principal is that when the protein is   activated it  undergoes a conformational change and this should be   indicated by a  change in the FRET-signal. The second approach utilizes   a SAMS-peptide   with fluorescent proteins fused to each end. The  SAMS-peptide will be  phosphorylated by the active SNF1-complex and  undergo a conformational  change that again will be indicated by the  FRET-signal.<br>  
   <br>  
   <br>  
-
                                Chalmers is a pioneer in Europe in the field of   start-up   companies, and has built up an efficient system of innovation   in order   to find applications in research into the formation of   companies. <br>  
+
                                The long term ambition of this project it is to use the  results in   the pharmaceutical industry when performing high-throughput   screening  for new substances or finding the correct drug  concentrations to use.  The yeast cells with the modified SNF1-complex   can be moved through a  micro-fluidic system, gradually exposing them  to an array of substances   or a concentration gradient thereby easily   finding at which  concentration or by what substance the cells are   stressed. <br>  
   <br>  
   <br>  
-
                                Chalmers has great aspirations to develop continually   by   opening doors to the outside world and by thinking innovatively. We    have a close and productive collaboration with business and society at    large. In this way we reinforce our own development, at the same time   as   we contribute to the growth of society.<br>  
+
                              As of present we have purified the plasmid backbones that   will be   used together with fusion protein insert to transfect the   yeast cells.   The fusion protein primers has arrived and we will start  working with   the fusion PCR as of this week. We have completed the 3D   models of the   fusion proteins and the results look very promising, we   will soon be  able to present docking predictions with the complex.<br>  
   <br>  
   <br>  
-
                                Chalmers was founded in  1829 as a result of a donation from  the director of the Swedish East  India Company, William Chalmers. His  motto, and ours, is:<br>  
+
 
-
  <br>  
+
<div id="myTitle">Introduction </div>
-
                                Avancez!</p>  
+
                             
-
                 
+
                       
-
                         
+
                       
-
</div>
+
                            <p><a href="http://2010.igem.org/Team:Gothenburg-Sweden/About_us"><img src="http://2010.igem.org/wiki/images/1/1a/Team-gbg.jpg" alt="" align="left" width="174" height="132" style="margin:0 20px 0 0"/> </a><p><span class="style9"><strong>Who we are?</strong> <br/>
-
                              
+
                            Left to right: Per Sunnerhagen, Markus Wilhelmsson, Malin Lindén, Karl Almén Burman, Adnan Kadic, Katarina Risö, Peidi Liu, Goutham Vemuri, Lokeshwaran Manoharan, Kemal Sanli, Julia Fransson </span><br /><br />  
 +
                            </p>  
 +
                            <p>&nbsp;</p>
 +
                            <p><strong>What we are doing? </strong><br>
 +
                            Synthetic readout of cellular stress</p>  
 +
                             <p >The cellular stress is sensed  by a key protein called  AMP-activated  protein kinase (AMPK). The AMPK protein  complex is  conserved among all  eukaryotes, including yeast, plants and humans. </p>
 +
                       
                    
                    
-
+
                </div> </div>
-
                     
+
-
                          <div id="myTitle">Supervisors</div>  
+
-
                         
+
-
                             
+
-
<td><table width="560" border="1">
+
-
                                      <tr>
+
-
                                        <td width="145" rowspan="2" valign="top"><span class="style8"><img src="http://2010.igem.org/wiki/images/e/eb/Goutham.jpg" alt="" width="133" height="200" class="img1" /></span></td>
+
-
                                        <td width="174" height="59" valign="top"><table width="150" border="5" cellpadding="0" cellspacing="0">
+
-
                                            <tr>
+
-
                                              <td><span class="style9">Goutham Vemuri</span></td>
+
-
                                            </tr>
+
-
                                            <tr>
+
-
                                              <td><span class="style11">supervisor</span></td>
+
-
                                            </tr>
+
-
                                        </table></td>
+
-
                                      </tr>
+
-
                                      <tr>
+
-
                                        <td valign="top"><p>&nbsp;</p>
+
-
                                        </td>
+
-
                                        <td width="214" valign="top"><p class="style5">&nbsp;</p>
+
-
                                  </table>
+
-
<p>&nbsp;</p>
+
-
<table width="560" border="1">
+
-
                                      <tr>
+
-
                                        <td width="145" rowspan="2" valign="top"><span class="style8"><img src="http://2010.igem.org/wiki/images/3/32/Per.jpg" alt="" width="133" height="200" class="img1" /></span></td>
+
-
                                        <td width="174" height="59" valign="top"><table width="150" border="5" cellpadding="0" cellspacing="0">
+
-
                                            <tr>
+
-
                                              <td><span class="style9">Per Sunnerhagen </span></td>
+
-
                                            </tr>
+
-
                                            <tr>
+
-
                                              <td><span class="style11">supervisor</span></td>
+
-
                                            </tr>
+
-
                                        </table></td>
+
-
                                      </tr>
+
-
                                      <tr>
+
-
                                        <td valign="top"><p>&nbsp;</p></td>
+
-
                                        <td width="214" valign="top"><p class="style5">&nbsp;</p>
+
-
                                  </table>
+
-
<p>&nbsp;</p>
+
-
<table width="560" border="1">
+
-
                                      <tr>
+
-
                                        <td width="145" rowspan="2" valign="top"><span class="style8"><img src="http://2010.igem.org/wiki/images/8/88/Markus.jpg" alt="" width="133" height="200" class="img1" /></span></td>
+
-
                                        <td width="169" height="59" valign="top"><table width="172" border="5" cellpadding="0" cellspacing="0">
+
-
                                            <tr>
+
-
                                              <td width="136"><span class="style9">Marcus Wilhelmsson  </span></td>
+
-
                                            </tr>
+
-
                                            <tr>
+
-
                                              <td><span class="style11">supervisor</span></td>
+
-
                                            </tr>
+
-
                                        </table></td>
+
-
                                      </tr>
+
-
                                      <tr>
+
-
                                        <td valign="top"><p>&nbsp;</p></td>
+
-
                                        <td width="219" valign="top"><p class="style5">&nbsp;</p>
+
-
                                  </table>
+
-
  <td>&nbsp;</td>
+
-
</tr>
+
-
<tr></tr>
+
-
<tr>
+
-
  <td>&nbsp;</td>
+
-
  </tr>
+
-
+
-
                       
+
-
                     
+
-
+
-
               
+
-
                            <div id="myTitle">Students</div>  
+
-
                           
+
-
                             
+
-
  <table width="560" border="1">
+
-
                                <tr>
+
-
                                  <td width="160" rowspan="2" valign="top"><span class="style8"><img src="http://2010.igem.org/wiki/images/d/d0/Malin.jpg" alt="" width="133" height="200" class="img1" /></span></td>
+
-
                                  <td width="167" height="59" valign="top"><table width="150" border="5" cellpadding="0" cellspacing="0">
+
-
                                      <tr>
+
-
                                        <td><span class="style9">Malin Linden</span></td>
+
-
                                      </tr>
+
-
                                      <tr>
+
-
                                        <td><span class="style11">student</span></td>
+
-
                                      </tr>
+
-
                                  </table></td>
+
-
                                </tr>
+
-
                                <tr>
+
-
                                  <td valign="top">
+
-
    <p><span class="style5">Age:</span></p>
+
-
                                      <p><span class="style5"><strong>Nationality:</strong></span></p>
+
-
                                    <p><span class="style5"><strong>Academic background:</strong></span></p>
+
-
                                    <p><span class="style5"><strong>Current studies:</strong></span></p></td>
+
-
                                  <td width="266" valign="top"><p class="style5">24</p>
+
-
                                      <p class="style5">Swedish</p>
+
-
                                    <p class="style5">B sc. Biotecnology</p>
+
-
                                    <p class="style5">Exchange year at University of Minnesota,</p>
+
-
                                  <td width="203">&nbsp;</td>
+
-
                                </tr>
+
-
                                        <tr>
+
-
                                          <td><span class="style5">MS Biotecnology</span></td>
+
-
                                        </tr>
+
-
      </table>
+
-
                               
+
-
                              </table>
+
-
      <p>&nbsp;</p>
+
-
      <table width="560" border="1">
+
-
                                <tr>
+
-
                                  <td width="145" rowspan="2" valign="top"><span class="style8"><img src="http://2010.igem.org/wiki/images/7/7f/Katarina.jpg" alt="" width="133" height="200" class="img1" /></span></td>
+
-
                                  <td width="151" height="59" valign="top"><table width="150" border="5" cellpadding="0" cellspacing="0">
+
-
                                      <tr>
+
-
                                        <td><span class="style9">Katarina Risö </span></td>
+
-
                                      </tr>
+
-
                                      <tr>
+
-
                                        <td><span class="style11">student</span></td>
+
-
                                      </tr>
+
-
                                  </table></td>
+
-
                                  <td width="237">&nbsp;</td>
+
-
                                </tr>
+
-
                                <tr>
+
-
                                  <td colspan="2" valign="top"><table width="368" height="219" border="1">
+
-
                                    <tr>
+
-
                                      <td><span class="style4">Age:</span></td>
+
-
                                      <td><span class="style5">24</span></td>
+
-
                                    </tr>
+
-
                                    <tr>
+
-
                                      <td><strong>Nationality:</strong></td>
+
-
                                      <td><span class="style5">Swedish</span></td>
+
-
                                    </tr>
+
-
                                    <tr>
+
-
                                      <td><strong>Academic background:</strong></td>
+
-
                                      <td><span class="style5">B sc. Chemical Engineering</span></td>
+
-
                                    </tr>
+
-
                                    <tr>
+
-
                                      <td><strong>Current studies:</strong></td>
+
-
                                      <td><span class="style5">MS Chemistry and Biosciences</span></td>
+
-
                                    </tr>
+
-
                                    <tr>
+
-
                                      <td>&nbsp;</td>
+
-
                                      <td>&nbsp;</td>
+
-
                                    </tr>
+
-
                                  </table>
+
-
                                    </td>
+
-
                                  </tr>
+
-
                              </table>
+
-
    <p>&nbsp;</p>
+
-
    <table width="560" border="1">
+
-
                                <tr>
+
-
                                  <td width="145" rowspan="2" valign="top"><span class="style8"><img src="http://2010.igem.org/wiki/images/a/a7/Peidi.jpg" alt="" width="133" height="200" class="img1" /></span></td>
+
-
                                  <td width="151" height="59" valign="top"><table width="150" border="5" cellpadding="0" cellspacing="0">
+
-
                                      <tr>
+
-
                                        <td><span class="style9">Peidi Liu</span></td>
+
-
                                      </tr>
+
-
                                      <tr>
+
-
                                        <td><span class="style11">student</span></td>
+
-
                                      </tr>
+
-
                                  </table></td>
+
-
                                  <td width="237">&nbsp;</td>
+
-
                                </tr>
+
-
                                <tr>
+
-
                                  <td valign="top"><p><span class="style4">Age:</span></p>
+
-
                                      <p><span class="style5"><strong>Nationality:</strong></span></p>
+
-
                                    <p><span class="style5"><strong>Academic background:</strong></span></p>
+
-
                                    <p><span class="style5"><strong>Current studies:</strong></span></p></td>
+
-
                                  <td valign="top"><p class="style5">24</p>
+
-
                                      <p class="style5">Chinese</p>
+
-
                                    <p class="style5">Electronic Engineering</p>
+
-
                                    <p><span class="style5">MS Bioinformatics</span></p></td>
+
-
                                </tr>
+
-
                              </table>
+
-
    <p>&nbsp;</p>
+
-
    <table width="560" border="1">
+
-
                                <tr>
+
-
                                  <td width="145" rowspan="2" valign="top"><span class="style8"><img src="http://2010.igem.org/wiki/images/8/80/Adnan.jpg" alt="" width="133" height="200" class="style5" /></span></td>
+
-
                                  <td width="151" height="59" valign="top"><table width="150" border="5" cellpadding="0" cellspacing="0">
+
-
                                      <tr>
+
-
                                        <td><span class="style9">Adnan Kadic</span></td>
+
-
                                      </tr>
+
-
                                      <tr>
+
-
                                        <td><span class="style11">student</span></td>
+
-
                                      </tr>
+
-
                                  </table></td>
+
-
                                  <td width="237">&nbsp;</td>
+
-
                                </tr>
+
-
                                <tr>
+
-
                                  <td height="141" valign="top"><p><span class="style4">Age:</span></p>
+
-
                                      <p><span class="style5"><strong>Nationality:</strong></span></p>
+
-
                                    <p><span class="style5"><strong>Academic background:</strong></span></p>
+
-
                                  <p><span class="style5"><strong>Current studies:</strong></span></p></td>
+
-
                                  <td valign="top"><p class="style5">24</p>
+
-
                                      <p class="style5">Bosnian</p>
+
-
                                    <p class="style5">B sc. Biotecnology</p>
+
-
                                    <p><span class="style5">MS Chemistry and Biosciences </span></p></td>
+
-
                                </tr>
+
-
                              </table>
+
-
    <p>&nbsp;</p>
+
-
    <table width="560" border="1">
+
-
                                <tr>
+
-
                                  <td width="145" rowspan="2" valign="top"><span class="style8"><img src="http://2010.igem.org/wiki/images/e/ea/Lokesh.jpg" alt="" width="133" height="200" class="style5" /></span></td>
+
-
                                  <td width="151" height="59" valign="top"><table width="150" border="5" cellpadding="0" cellspacing="0">
+
-
                                      <tr>
+
-
                                        <td><span class="style9">Lokeshwaran Manoharan </span></td>
+
-
                                      </tr>
+
-
                                      <tr>
+
-
                                        <td><span class="style11">student</span></td>
+
-
                                      </tr>
+
-
                                  </table></td>
+
-
                                  <td width="237">&nbsp;</td>
+
-
                                </tr>
+
-
                                <tr>
+
-
                                  <td height="141" valign="top"><p><span class="style4">Age:</span></p>
+
-
                                      <p><span class="style5"><strong>Nationality:</strong></span></p>
+
-
                                    <p><span class="style5"><strong>Academic background:</strong></span></p>
+
-
                                  <p><span class="style5"><strong>Current studies:</strong></span></p></td>
+
-
                                  <td valign="top"><p class="style5">23</p>
+
-
                                      <p class="style5">Indian</p>
+
-
                                    <p class="style5">B.Tech Ind Biotechnology</p>
+
-
                                    <p class="style5">MS Bioinformatics</p> 
+
-
                                  <p>&nbsp;</p></td>
+
-
                                </tr>
+
-
                              </table>
+
-
    <p>&nbsp;</p>
+
-
    <table width="560" border="1">
+
-
                        <tr>
+
-
                          <td width="145" rowspan="2" valign="top"><span class="style8"><img src="http://2010.igem.org/wiki/images/0/01/Kemal.jpg" alt="" width="133" height="200" class="style5" /></span></td>
+
-
                          <td width="151" height="59" valign="top"><table width="150" border="5" cellpadding="0" cellspacing="0">
+
-
                              <tr>
+
-
                                <td><span class="style9">Kemal Sanli  </span></td>
+
-
                              </tr>
+
-
                              <tr>
+
-
                                <td><span class="style11">student</span></td>
+
-
                              </tr>
+
-
                          </table></td>
+
-
                          <td width="237">&nbsp;</td>
+
-
                        </tr>
+
-
                        <tr>
+
-
                          <td height="139" valign="top"><p><span class="style4">Age:</span></p>
+
-
                              <p><span class="style5"><strong>Nationality:</strong></span></p>
+
-
                            <p><span class="style5"><strong>Academic background:</strong></span></p>
+
-
                          <p><span class="style5"><strong>Current studies:</strong></span></p></td>
+
-
                          <td valign="top"><p class="style5">24</p>
+
-
                              <p class="style5">Turkish</p>
+
-
                            <p class="style5">B sc. Molecular Biology and Genetics </p>
+
-
                            <p class="style5">MS Bioinformatics </p></td>
+
-
                        </tr>
+
-
                      </table>  
+
-
              <p>&nbsp;</p>
+
-
              <table width="560" border="1">
+
-
                        <tr>
+
-
                          <td width="145" rowspan="2" valign="top"><span class="style8"><img src="http://2010.igem.org/wiki/images/4/40/Karl.jpg" alt="" width="133" height="200" class="style5" /></span></td>
+
-
                          <td width="151" height="59" valign="top"><table width="150" border="5" cellpadding="0" cellspacing="0">
+
-
                              <tr>
+
-
                                <td><span class="style9">Karl Almén Burman </span></td>
+
-
                              </tr>
+
-
                              <tr>
+
-
                                <td><span class="style11">student</span></td>
+
-
                              </tr>
+
-
                          </table></td>
+
-
                          <td width="237">&nbsp;</td>
+
-
                        </tr>
+
-
                        <tr>
+
-
                          <td height="141" valign="top"><p><span class="style4">Age:</span></p>
+
-
                              <p><span class="style5"><strong>Nationality:</strong></span></p>
+
-
                            <p><span class="style5"><strong>Academic background:</strong></span></p>
+
-
                          <p><span class="style5"><strong>Current studies:</strong></span></p></td>
+
-
                          <td valign="top"><p class="style5">23</p>
+
-
                              <p class="style5">Swedish</p>
+
-
                            <p class="style5">B sc. Biotechnology </p>
+
-
                            <p><span class="style5">MS Biotechnology </span></p></td>
+
-
                        </tr>
+
-
                      </table>  
+
-
              <p>&nbsp;</p>
+
-
              <table width="560" border="1">
+
-
                        <tr>
+
-
                          <td width="145" rowspan="2" valign="top"><span class="style8"><img src="http://2010.igem.org/wiki/images/e/e8/Julia.jpg" alt="" width="133" height="200" class="style5" /></span></td>
+
-
                          <td width="151" height="59" valign="top"><table width="150" border="5" cellpadding="0" cellspacing="0">
+
-
                              <tr>
+
-
                                <td><span class="style9">Julia Fransson </span></td>
+
-
                              </tr>
+
-
                              <tr>
+
-
                                <td><span class="style11">student</span></td>
+
-
                              </tr>
+
-
                          </table></td>
+
-
                          <td width="237">&nbsp;</td>
+
-
                        </tr>
+
-
                        <tr>
+
-
                          <td height="172" valign="top"><p><span class="style4">Age:</span></p>
+
-
                              <p><span class="style5"><strong>Nationality:</strong></span></p>
+
-
                            <p><span class="style5"><strong>Academic background:</strong></span></p>
+
-
                            <p><span class="style5"><strong>Current studies:</strong></span></p></td>
+
-
                          <td valign="top"><p class="style5">22</p>
+
-
                              <p class="style5">Swedish</p>
+
-
                              <p class="style5">B sc. Biotechnology </p>
+
-
                              <p class="style5"> Exchange year through Unitech International</p>
+
-
                              <p class="style5">&nbsp;</p></td>
+
-
                        </tr>
+
-
                      </table>  
+
-
          </td>
+
-
  </tr><tr><td>&nbsp;</td>
+
-
  </tr><tr><td>&nbsp;</td>
+
-
  </tr>
+
-
</div>
+
<div id="right">
-
</div>
+
-
 
+
-
<div id="right">
+
                 <div class="Title3">Lab Notes</div>
                 <div class="Title3">Lab Notes</div>
                            
                            
Line 991: Line 707:
              
              
                                  
                                  
-
      
+
     </div>   
-
  </div>
+
  <div class="Title3">News</div>
 +
                              <div id="news">
 +
                                    <p>Göteborgs-Posten, a major daily newspaper in Sweden with the second largest national circulation, published an article involving the interview with our team...
 +
                                    <br><br> </p>
 +
<div class="read"><a href="#">read more</a></div>
 +
                                          <div style="clear: both"></div>
 +
                                </div>
 +
                     
 +
 
 +
 +
</div>
 +
   <div id="myfooter">  <p>  <a href="#">Home</a> | <a href="#">CTH</a> | <a href="#">Sponsors</a> | <a href="#">About Us</a> | <a href="#">Contact Us</a></p> <p>Chalmers University of Technology, Gothenburg, SWEDEN</p>
   <div id="myfooter">  <p>  <a href="#">Home</a> | <a href="#">CTH</a> | <a href="#">Sponsors</a> | <a href="#">About Us</a> | <a href="#">Contact Us</a></p> <p>Chalmers University of Technology, Gothenburg, SWEDEN</p>

Revision as of 08:52, 30 September 2010

Chalmers University of Technology

Team
FUSS

CHALMERS UNIVERSITY OF TECHNOLOGY, GOTHENBURG

We are a team of 8 students from Chalmers University of Technology who will represent Gothenburg, SWEDEN in this year’s iGEM competition. We have started with a promising idea that combines cutting edge technologies available in the field of Synthetic Biology. Our research basically aims to constructing an optical reporter mechanism for cellular stress by tagging the stress activated SNF1 complex in yeast with fluorescent markers.

The project is performed with two main experimental pathways: Both experimental setups will utilize FRET to visualize the conformational change that is the result SNF1 activation. The first approach consists of fusing fluorescent two proteins, EYFP and ECFP, with the SNF1 complex. The principal is that when the protein is activated it undergoes a conformational change and this should be indicated by a change in the FRET-signal. The second approach utilizes a SAMS-peptide with fluorescent proteins fused to each end. The SAMS-peptide will be phosphorylated by the active SNF1-complex and undergo a conformational change that again will be indicated by the FRET-signal.

The long term ambition of this project it is to use the results in the pharmaceutical industry when performing high-throughput screening for new substances or finding the correct drug concentrations to use. The yeast cells with the modified SNF1-complex can be moved through a micro-fluidic system, gradually exposing them to an array of substances or a concentration gradient thereby easily finding at which concentration or by what substance the cells are stressed.

As of present we have purified the plasmid backbones that will be used together with fusion protein insert to transfect the yeast cells. The fusion protein primers has arrived and we will start working with the fusion PCR as of this week. We have completed the 3D models of the fusion proteins and the results look very promising, we will soon be able to present docking predictions with the complex.

Introduction

Who we are?
Left to right: Per Sunnerhagen, Markus Wilhelmsson, Malin Lindén, Karl Almén Burman, Adnan Kadic, Katarina Risö, Peidi Liu, Goutham Vemuri, Lokeshwaran Manoharan, Kemal Sanli, Julia Fransson


 

What we are doing?
Synthetic readout of cellular stress

The cellular stress is sensed by a key protein called AMP-activated protein kinase (AMPK). The AMPK protein complex is conserved among all eukaryotes, including yeast, plants and humans.

Home | CTH | Sponsors | About Us | Contact Us

Chalmers University of Technology, Gothenburg, SWEDEN