Team:Gothenburg-Sweden/chalmers

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<html>
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<title>iGEM-Gothenburg</title>
<title>iGEM-Gothenburg</title>
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                                        <li><a href="https://2010.igem.org/Team:Gothenburg-Sweden" >Home</a></li>
<li><a href="https://2010.igem.org/Team:Gothenburg-Sweden/chalmers" >Chalmers, GBG</a></li>
<li><a href="https://2010.igem.org/Team:Gothenburg-Sweden/chalmers" >Chalmers, GBG</a></li>
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<h1>Chalmers University of Technology</h1>
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<p style="font-size:160%;font-weight:bold"><i>iGEM team - Sweden, Gothenburg</i></p>
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<p>We are a team of 8 students from Chalmers University of Technology who will represent Gothenburg, SWEDEN in this year’s IGEM competition. We have started with a promising idea that combines the cutting edge technologies available in the field of Synthetic Biology. Our research basically aims to constructing a reporter mechanism for cellular stress in yeast. This is done by tagging protein and peptides affiliated with the stress activated SNF1 complex with fluorescent markers.</p>
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<p>The project is executed through two main experimental pathways. Both experimental setups will utilize FRET to visualize the conformational change that is the result of the activation of the SNF1 protein. The first approach consists of creating a fusion protein consisting of the SNF1 protein and two fluorescent proteins, namely EYFP and ECFP. The idea is that when the protein is activated it undergoes a conformational change and a FRET-signal will be visible. The second approach utilizes a SAMS-peptide with fluorescent proteins fused to each end. The SAMS-peptide will be phosphorylated by the active SNF1-complex and will undergo a conformational change that will be visible due to the fluorescent tags.</p>  
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<p>The long term ambition of this project it is to use the results in the pharmaceutical industry when performing high-throughput screening for new substances or finding the correct drug concentrations to use. The yeast cells with the modified SNF-complex can be moved through a micro-fluidic system, gradually exposing them to an array of substances or a concentration gradient and easily finding out at which concentration or substance that the cells are stressed.</p>
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<p>We are a team of 8 students from Chalmers University of Technology who   will represent Gothenburg, SWEDEN in this year’s IGEM competition. IGEM  stands for International Genetically Engineered Machines and is a  competition based upon interdisciplinary collaboration of students on a  Synthetic Biology project. The competition is held in MIT, Boston and is  open to all universities from various countries world-wide. There are  180 teams participating this year with about 2000 students in total.  We have started with a promising idea that combines the cutting edge   technologies available in the field of Synthetic Biology. Our research   basically includes the specification and designing of a biological  system followed by the application of Molecular Biology techniques to  build and test it experimentally. The premise of the competition for the  students will be to learn engineering approaches and tools to organize,  model, and assemble complex systems and to immerse themselves in  applied molecular biology.   In the project, we are investigating a biological phenomenon that is a  part of insulin uptake mechanism, widely studied in Diabetic research.   Our endeavor in the study is to visualize a part of the mechanism by  making use of the Nobel Prize winning idea of the Green Fluorescent  Proteins (GFPs). Hopefully, the project will provide us with auspicious  outcomes to further improve the study of the disease.</p>
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<p>As of present we have constructed and ordered primers for all fusion proteins that will be tested if they give a FRET signal in yeast. We are also working on 3D models of the fusion protein and will soon be able to present docking predictions with the complex.
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Latest revision as of 11:27, 2 July 2010

iGEM-Gothenburg

Contact Us

Chalmers University of Technology

We are a team of 8 students from Chalmers University of Technology who will represent Gothenburg, SWEDEN in this year’s IGEM competition. We have started with a promising idea that combines the cutting edge technologies available in the field of Synthetic Biology. Our research basically aims to constructing a reporter mechanism for cellular stress in yeast. This is done by tagging protein and peptides affiliated with the stress activated SNF1 complex with fluorescent markers.

The project is executed through two main experimental pathways. Both experimental setups will utilize FRET to visualize the conformational change that is the result of the activation of the SNF1 protein. The first approach consists of creating a fusion protein consisting of the SNF1 protein and two fluorescent proteins, namely EYFP and ECFP. The idea is that when the protein is activated it undergoes a conformational change and a FRET-signal will be visible. The second approach utilizes a SAMS-peptide with fluorescent proteins fused to each end. The SAMS-peptide will be phosphorylated by the active SNF1-complex and will undergo a conformational change that will be visible due to the fluorescent tags.

The long term ambition of this project it is to use the results in the pharmaceutical industry when performing high-throughput screening for new substances or finding the correct drug concentrations to use. The yeast cells with the modified SNF-complex can be moved through a micro-fluidic system, gradually exposing them to an array of substances or a concentration gradient and easily finding out at which concentration or substance that the cells are stressed.

As of present we have constructed and ordered primers for all fusion proteins that will be tested if they give a FRET signal in yeast. We are also working on 3D models of the fusion protein and will soon be able to present docking predictions with the complex.

© 2010 Chalmers University of Technology, All rights reserved.

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