http://2010.igem.org/wiki/index.php?title=Team:Georgia_State/Producingtheantigen&feed=atom&action=historyTeam:Georgia State/Producingtheantigen - Revision history2024-03-28T15:20:46ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Georgia_State/Producingtheantigen&diff=206996&oldid=prevAlalani at 03:17, 28 October 20102010-10-28T03:17:47Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Designing the Antigen''' ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Designing the Antigen''' ==</div></td></tr>
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</table>Alalanihttp://2010.igem.org/wiki/index.php?title=Team:Georgia_State/Producingtheantigen&diff=206985&oldid=prevAlalani at 03:17, 28 October 20102010-10-28T03:17:30Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Designing the Antigen''' ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Designing the Antigen''' ==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In order to design an antigen we compared previously isolated and sequenced Influenza A H1N1 strains. We were able to identify a conserved region that comprised of the Hemagglutinin globular head which is formed by a disulfide bond between two cysteins. This region of the virus has been shown to produce an immune response. This is important since we plan on being able to use this as a potential vaccine. In order to identify the location of the cysteins within the sequence, we converted the nucleotide sequence to the amino acid sequence. The cysteins were identified, and our part was designed to include this region. The next step was to design this part to fit the registry standard. Two unwanted restriction sites were identified. In order to eliminate these sites, we did a nucleotide swap while maintaining the same amino acid sequence. Following the above steps, the part has been designed to be compatible with all assembly standards.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In order to design an antigen we compared previously isolated and sequenced Influenza A H1N1 strains. We were able to identify a conserved region that comprised of the Hemagglutinin globular head which is formed by a disulfide bond between two cysteins. This region of the virus has been shown to produce an immune response. This is important since we plan on being able to use this as a potential vaccine. In order to identify the location of the cysteins within the sequence, we converted the nucleotide sequence to the amino acid sequence. The cysteins were identified, and our part was designed to include this region. The next step was to design this part to fit the registry standard. Two unwanted restriction sites were identified. In order to eliminate these sites, we did a nucleotide swap while maintaining the same amino acid sequence. Following the above steps, the part has been designed to be compatible with all assembly standards.</div></td></tr>
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</table>Alalanihttp://2010.igem.org/wiki/index.php?title=Team:Georgia_State/Producingtheantigen&diff=206952&oldid=prevAlalani at 03:16, 28 October 20102010-10-28T03:16:51Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Designing the Antigen''' ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Designing the Antigen''' ==</div></td></tr>
</table>Alalanihttp://2010.igem.org/wiki/index.php?title=Team:Georgia_State/Producingtheantigen&diff=206925&oldid=prevAlalani at 03:16, 28 October 20102010-10-28T03:16:09Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Designing the Antigen''' ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Designing the Antigen''' ==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In order to design an antigen we compared previously isolated and sequenced Influenza A H1N1 strains. We were able to identify a conserved region that comprised of the Hemagglutinin globular head which is formed by a disulfide bond between two cysteins. This region of the virus has been shown to produce an immune response. This is important since we plan on being able to use this as a potential vaccine. In order to identify the location of the cysteins within the sequence, we converted the nucleotide sequence to the amino acid sequence. The cysteins were identified, and our part was designed to include this region. The next step was to design this part to fit the registry standard. Two unwanted restriction sites were identified. In order to eliminate these sites, we did a nucleotide swap while maintaining the same amino acid sequence. Following the above steps, the part has been designed to be compatible with all assembly standards.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In order to design an antigen we compared previously isolated and sequenced Influenza A H1N1 strains. We were able to identify a conserved region that comprised of the Hemagglutinin globular head which is formed by a disulfide bond between two cysteins. This region of the virus has been shown to produce an immune response. This is important since we plan on being able to use this as a potential vaccine. In order to identify the location of the cysteins within the sequence, we converted the nucleotide sequence to the amino acid sequence. The cysteins were identified, and our part was designed to include this region. The next step was to design this part to fit the registry standard. Two unwanted restriction sites were identified. In order to eliminate these sites, we did a nucleotide swap while maintaining the same amino acid sequence. Following the above steps, the part has been designed to be compatible with all assembly standards.</div></td></tr>
</table>Alalanihttp://2010.igem.org/wiki/index.php?title=Team:Georgia_State/Producingtheantigen&diff=206914&oldid=prevAlalani: New page: == '''Designing the Antigen''' == In order to design an antigen we compared previously isolated and sequenced Influenza A H1N1 strains. We were able to identify a conserved region tha...2010-10-28T03:15:49Z<p>New page: == '''Designing the Antigen''' == In order to design an antigen we compared previously isolated and sequenced Influenza A H1N1 strains. We were able to identify a conserved region tha...</p>
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== '''Designing the Antigen''' ==<br />
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In order to design an antigen we compared previously isolated and sequenced Influenza A H1N1 strains. We were able to identify a conserved region that comprised of the Hemagglutinin globular head which is formed by a disulfide bond between two cysteins. This region of the virus has been shown to produce an immune response. This is important since we plan on being able to use this as a potential vaccine. In order to identify the location of the cysteins within the sequence, we converted the nucleotide sequence to the amino acid sequence. The cysteins were identified, and our part was designed to include this region. The next step was to design this part to fit the registry standard. Two unwanted restriction sites were identified. In order to eliminate these sites, we did a nucleotide swap while maintaining the same amino acid sequence. Following the above steps, the part has been designed to be compatible with all assembly standards.</div>Alalani