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Team:Georgia State/Notebook
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*Also plated ≈100mL (remaining amount) of cells onto LB plates not containing ampicillin. | *Also plated ≈100mL (remaining amount) of cells onto LB plates not containing ampicillin. | ||
*Spread plates with hockey stick and placed in 37°C at 7:35. | *Spread plates with hockey stick and placed in 37°C at 7:35. | ||
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Revision as of 16:18, 15 July 2010
Contents |
Notebook
July 8, 2010
Angie, Kendra, Melissa, Nishedh, Alykhan
- Diluted pichia cells in a volume of 50mL to a OD600 = .186+.201
- Transform pars 12E and 12M into E. coli
July 7, 2010
Joe, Kendra, Angie
- Replated colonies from 9A RFP plate (3 plates).
- Colonies on broth 100 µL for 9A but no fluorescence observed
- 9A survivor colonies replated onto 10 µg/mL ampicillin plates and incubated at 30°C
- Mother plate in fridge
- Made 1000mL YPD
- Growing P. anomala in YPD broth for competent cells protocol
July 6, 2010
Dan
- Replated E. coli culture
Alykhan
- Transformation 9A, 8I (digested) & GFP.
July 2, 2010
Alykhan and Virginia
- DNA purification and ligation
- Replated E. coli culture
July 1, 2010
Joe
- Plated transformed cells containing either GFP (control) or iGEM part onto LB plates with 10µg/mL ampicillin (2 plates each, 10mL or 100mL)
- Also plated ≈100mL (remaining amount) of cells onto LB plates not containing ampicillin.
- Spread plates with hockey stick and placed in 37°C at 7:35.
