"
Team:GeorgiaTech/Ethics and Safety
From 2010.igem.org
| Line 1: | Line 1: | ||
| - | <div id="container"><div id="header">[[Image: | + | <div id="container"><div id="header">[[Image:bannerhome.jpg|965px|logo]] |
<html> | <html> | ||
<style type="text/css"> | <style type="text/css"> | ||
| Line 16: | Line 16: | ||
table | table | ||
{ | { | ||
| - | background-color: # | + | background-color: #964141; |
font-color: white; | font-color: white; | ||
| - | color: # | + | color: #964141; |
} | } | ||
| Line 25: | Line 25: | ||
#bodyContent | #bodyContent | ||
{ | { | ||
| - | background-color: | + | background-color: ; |
color: white; | color: white; | ||
} | } | ||
| Line 43: | Line 43: | ||
p | p | ||
{ | { | ||
| - | background-color: # | + | background-color: #964141; |
color: white; | color: white; | ||
} | } | ||
| Line 76: | Line 76: | ||
<center>Safety Training</center> | <center>Safety Training</center> | ||
| - | <center><p><b><FONT COLOR="#FFFFFF">Team Member</FONT</b></p> | + | <center><p><b><FONT COLOR="#FFFFFF">Team Member</FONT></b></p> |
<table border="2" cellpadding ="5" cellspacing = "5"> | <table border="2" cellpadding ="5" cellspacing = "5"> | ||
<tr><th><FONT COLOR="#FFFFFF">Atta Hassan</FONT></th><th><FONT COLOR="#FFFFFF">Marc Pline's Bio Safety Training</FONT></th></tr> | <tr><th><FONT COLOR="#FFFFFF">Atta Hassan</FONT></th><th><FONT COLOR="#FFFFFF">Marc Pline's Bio Safety Training</FONT></th></tr> | ||
| Line 106: | Line 106: | ||
<p><center><font color="#FFFFFF" size=5><b>Questions About Ethics and Safety</b></font></center></p> | <p><center><font color="#FFFFFF" size=5><b>Questions About Ethics and Safety</b></font></center></p> | ||
| + | |||
<table width="965" border="0" cellpadding="0" cellspacing="0"> | <table width="965" border="0" cellpadding="0" cellspacing="0"> | ||
<tr> | <tr> | ||
| Line 111: | Line 112: | ||
</tr> | </tr> | ||
</table> | </table> | ||
| - | <table | + | |
| - | + | <table width="800" border="0" cellpadding="2" cellspacing="10"> | |
| - | + | ||
| - | + | ||
<tr> | <tr> | ||
| + | <td bgcolor="#964141" width="800"><font color="#FFFFFF"><b><p>1. Would any of your project ideas raise safety issues in terms of:</p> | ||
| + | |||
<ul><font color="#FFFFFF"> | <ul><font color="#FFFFFF"> | ||
<li>researcher safety,</li> | <li>researcher safety,</li> | ||
<li>public safety, or</li> | <li>public safety, or</li> | ||
<li>environmental safety?</li> | <li>environmental safety?</li> | ||
| - | </ul> | + | </ul></b> |
<p>Our idea involves the insertion of the AOX gene from the sacred lotus, which redirects energy from the | <p>Our idea involves the insertion of the AOX gene from the sacred lotus, which redirects energy from the | ||
metabolic system to be released as heat in response to a cold-shock. The protein product produced is | metabolic system to be released as heat in response to a cold-shock. The protein product produced is | ||
| Line 130: | Line 131: | ||
used in a Biosafety Level 1 laboratory setting.</p> | used in a Biosafety Level 1 laboratory setting.</p> | ||
| - | <p>2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,</p> | + | <p><b>2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,</p> |
<ul><font color="#FFFFFF"> | <ul><font color="#FFFFFF"> | ||
<li>Did you document these issues in the Registry?</li> | <li>Did you document these issues in the Registry?</li> | ||
<li>How did you manage to handle the safety issue?</li> | <li>How did you manage to handle the safety issue?</li> | ||
<li>How could other teams learn from your experience?</li> | <li>How could other teams learn from your experience?</li> | ||
| - | </ul> | + | </ul></b> |
<p>We constructed a bacterial plasmid containing the AOX gene with a targeting sequence, OmpA, and | <p>We constructed a bacterial plasmid containing the AOX gene with a targeting sequence, OmpA, and | ||
promoter, HybB, which have no known biosafety issues.</p> | promoter, HybB, which have no known biosafety issues.</p> | ||
| - | <p>3. Is there a local biosafety group, committee, or review board at your institution?</p> | + | <p><b>3. Is there a local biosafety group, committee, or review board at your institution?</p> |
<ul><font color="#FFFFFF"> | <ul><font color="#FFFFFF"> | ||
| Line 146: | Line 147: | ||
<li>If no, which specific biosafety rules or guidelines do you have to consider in your | <li>If no, which specific biosafety rules or guidelines do you have to consider in your | ||
country?</li> | country?</li> | ||
| - | </ul> | + | </ul></b> |
<p>The Occupational Health and Safety Committee and the Environmental Health and Safety Office | <p>The Occupational Health and Safety Committee and the Environmental Health and Safety Office | ||
| Line 152: | Line 153: | ||
Biosafety Level 1 Research.</p> | Biosafety Level 1 Research.</p> | ||
| - | <p>4. Do you have any other ideas how to deal with safety issues that could be useful for future | + | <p><b>4. Do you have any other ideas how to deal with safety issues that could be useful for future |
iGEM competitions? How could parts, devices and systems be made even safer through | iGEM competitions? How could parts, devices and systems be made even safer through | ||
| - | biosafety engineering?</p> | + | biosafety engineering?</p></b> |
<p>Our group has discussed the sharp learning curve associated with the first year of a team’s experience | <p>Our group has discussed the sharp learning curve associated with the first year of a team’s experience | ||
Revision as of 15:34, 27 October 2010
Team Member
| Atta Hassan | Marc Pline's Bio Safety Training |
|---|---|
| Debika Mitra | MSE Safety Training |
| Amy Schwartz | Marc Pline's Bio Safety Training |
| Mitesh Agrawal | Marc Pline's Bio Safety Training |
| Monica Huynh | Marc Pline's Bio Safety Training |
| Margo Clark | Marc Pline's Bio Safety Training |
| Gita Mahmoudabadi | Marc Pline's Bio Safety Training |
| Christian Mandrycky | Marc Pline's Bio Safety Training |
| Sid Tantia | Marc Pline's Bio Safety Training |
| Scott Holmes | Marc Pline's Bio Safety Training |
Note: Marc Pline is from the GT School of Biology
1. Would any of your project ideas raise safety issues in terms of:
Our idea involves the insertion of the AOX gene from the sacred lotus, which redirects energy from the metabolic system to be released as heat in response to a cold-shock. The protein product produced is directed to the inner membrane of the cell, and not released into the environment. As our research is confined to in vitro usage at this point, and the components used have no known adverse effects on the environment, there is very little risk associated with a potential accident involving our project. The research pursued does not pose any known safety hazards to researchers, the public, or the environment. The strains of E. coli we utilized were non-pathogenic, standard lab strains that are safely used in a Biosafety Level 1 laboratory setting. 2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,
We constructed a bacterial plasmid containing the AOX gene with a targeting sequence, OmpA, and promoter, HybB, which have no known biosafety issues. 3. Is there a local biosafety group, committee, or review board at your institution?
The Occupational Health and Safety Committee and the Environmental Health and Safety Office of the Georgia Institute of Technology has approved Professor Eric Gaucher’s laboratory for Biosafety Level 1 Research. 4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? Our group has discussed the sharp learning curve associated with the first year of a team’s experience with iGEM. Although our institution offered, and we all attended, a biological laboratory safety class, there were many things we learned along the way, specific to research performed in the bacterial genetic engineering laboratory. We would propose a collaboration of iGEM members to produce an online, visual, one-hour introductory course on the basic biosafety issues that could arise during the iGEM research process. 
|
