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Team:GeorgiaTech/Biobricks
From 2010.igem.org
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<p><center><font color="#FFFFFF" size=5><b>BioBrick Constructs</b></font></center></p> | <p><center><font color="#FFFFFF" size=5><b>BioBrick Constructs</b></font></center></p> | ||
<center><img src="https://static.igem.org/mediawiki/2010/0/04/Biobrick_Constructs-1-.JPG" alt="construct" width="500" height="500"></center> | <center><img src="https://static.igem.org/mediawiki/2010/0/04/Biobrick_Constructs-1-.JPG" alt="construct" width="500" height="500"></center> | ||
| - | <p>There are several constructs we hope to submit as BioBricks to the Parts Registry. Construct (a) is a fusion of the HybB cold shock promoter, the Ompa signal peptide, and either of the two forms of AOX. Construct (b) with mRFP (red fluorescent protein) at the c-terminus of AOX will allow for imaging in the bacterial periplasm. To quantify the activity of the HyBb promoter at different | + | <p>There are several constructs we hope to submit as BioBricks to the Parts Registry. Construct (a) is a fusion of the HybB cold shock promoter, the Ompa signal peptide, and either of the two forms of AOX. Construct (b) with mRFP (red fluorescent protein) at the c-terminus of AOX will allow for imaging in the bacterial periplasm. To quantify the activity of the HyBb promoter at different temperatures, we will express mRFP using HyBb in construct (c). Note that the final BioBricks will be in the pSB1C3 assembly plasmid. </p> |
Revision as of 03:08, 3 September 2010
BioBricks also known as standard biological parts, are DNA sequences that when introduced into an organism, act as a component of a desired mechanism. For more information on BioBricks click here
There are several constructs we hope to submit as BioBricks to the Parts Registry. Construct (a) is a fusion of the HybB cold shock promoter, the Ompa signal peptide, and either of the two forms of AOX. Construct (b) with mRFP (red fluorescent protein) at the c-terminus of AOX will allow for imaging in the bacterial periplasm. To quantify the activity of the HyBb promoter at different temperatures, we will express mRFP using HyBb in construct (c). Note that the final BioBricks will be in the pSB1C3 assembly plasmid.
